2012
DOI: 10.1073/pnas.1207067109
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Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ

Abstract: The small RNA PcrZ (photosynthesis control RNA Z) of the facultative phototrophic bacterium Rhodobacter sphaeroides is induced upon a drop of oxygen tension with similar kinetics to those of genes for components of photosynthetic complexes. High expression of PcrZ depends on PrrA, the response regulator of the PrrB/ PrrA two-component system with a central role in redox regulation in R. sphaeroides. In addition the FnrL protein, an activator of some photosynthesis genes at low oxygen tension, is involved in re… Show more

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Cited by 58 publications
(92 citation statements)
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“…Our data show that this negative effect is (at least partly) due to a specific, Hfq-independent cleavage of RNase E in the 5= UTR of sinI mRNA. The Hfq-independent status of this cleavage does not exclude the involvement of an sRNA, since a trans-encoded, Hfq-independent sRNA was shown to regulate the expression of photosynthesis genes in the alphaproteobacterium Rhodobacter sphaeroides (65).…”
Section: Discussionmentioning
confidence: 99%
“…Our data show that this negative effect is (at least partly) due to a specific, Hfq-independent cleavage of RNase E in the 5= UTR of sinI mRNA. The Hfq-independent status of this cleavage does not exclude the involvement of an sRNA, since a trans-encoded, Hfq-independent sRNA was shown to regulate the expression of photosynthesis genes in the alphaproteobacterium Rhodobacter sphaeroides (65).…”
Section: Discussionmentioning
confidence: 99%
“…In order to construct bacterial strains with enhanced levels of CcsR RNAs, we made use of overexpression plasmids pRK4352 and pBBR4352 (4). A DNA fragment containing the promoterless RSP_6037-CcsR1-4 locus was PCR amplified, subcloned into the pDrive cloning vector (Qiagen), and ligated into the BamHI/EcoRI sites of pRK4352 after restriction with the corresponding enzymes.…”
Section: Methodsmentioning
confidence: 99%
“…The protein-coding sequence of flhR, together with the 5= untranslated region (UTR), was amplified with primers 2591ovfbam1 and 2591ovRkpn1 and introduced into the BamHI and KpnI sites of pRK4352 (4). The resulting plasmid, pRK_flhR, was transferred to wild-type R. sphaeroides 2.4.1 and an endogenous flhR deletion strain, and tetracycline-resistant clones were selected.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…In the bacterium Rhodobacter sphaeroides, which is facultatively capable of anoxygenic photosynthesis, the trans-acting sRNA PcrZ has a direct regulatory function in the formation of photosynthetic complexes (Mank et al, 2012). However, no transencoded sRNAs regulating oxygenic photosynthesis in plant chloroplasts or cyanobacteria have been described thus far.…”
Section: Introductionmentioning
confidence: 99%