Regulation of biofilm formation by non-coding RNA in prokaryotes

Mitra, Arindam; Mukhopadhyay, Suman

doi:10.1016/j.crphar.2022.100151

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…Several other sRNAs have been described as pleiotropic modulators of bacterial virulence, such as P. aeruginosa RsmZ, ReaL, ErsA, and SrbA (Liu et al 2022); Staphylococcus aureus RsaA and Teg49 (Menard et al 2022); and E. coli and Salmonella enterica McaS, OmrA, and OxyS (Mitra and Mukhopadhyay 2023). Therefore, whilst most of these sRNAs positively modulate bacterial virulence and biofilm formation, RIT11b was downregulated when B. cenocepacia was infecting C. elegans and its overexpression reduced bacterial virulence and biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, whilst most of these sRNAs positively modulate bacterial virulence and biofilm formation, RIT11b was downregulated when B. cenocepacia was infecting C. elegans and its overexpression reduced bacterial virulence and biofilm formation. OmrA and RydC, in Gram-negative bacteria, and AscicR and csRNA1-1, in Gram-positive bacteria, are some of the few sRNAs that also negatively modulate biofilm formation (Mitra and Mukhopadhyay 2023).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection

Pita

Feliciano

Leitão

2023

Appl Microbiol Biotechnol

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Small non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Despite the identification of hundreds of bacterial sRNAs, their roles on bacterial physiology and virulence remain largely unknown, as is the case of bacteria of the Burkholderia cepacia complex (Bcc). Bcc is a group of opportunistic pathogens with relatively large genomes that can cause lethal lung infections amongst cystic fibrosis (CF) patients. To characterise sRNAs expressed by Bcc bacteria when infecting a host, the nematode Caenorhabditis elegans was used as an infection model by the epidemic CF strain B. cenocepacia J2315. A total of 108 new and 31 previously described sRNAs with a predicted Rho independent terminator were identified, most of them located on chromosome 1. RIT11b, a sRNA downregulated under C. elegans infection conditions, was shown to directly affect B. cenocepacia virulence, biofilm formation, and swimming motility. RIT11b overexpression reduced the expression of the direct targets dusA and pyrC, involved in biofilm formation, epithelial cell adherence, and chronic infections in other organisms. The in vitro direct interaction of RIT11b with the dusA and pyrC messengers was demonstrated by electrophoretic mobility shift assays. To the best of our knowledge this is the first report on the functional characterization of a sRNA directly involved in B. cenocepacia virulence. Key points • 139 sRNAs expressed by B. cenocepacia during C. elegans infection were identified • The sRNA RIT11b affects B. cenocepacia virulence, biofilm formation, and motility • RIT11b directly binds to and regulates dusA and pyrC mRNAs

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection

Pita

Feliciano

Leitão

2023

Appl Microbiol Biotechnol

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“…The genetics and environmental signals contribute to the regulation of biofilm development and dispersion in bacteria [19]. Three main players' quorum sensing (QS), bis-(3 -5 )-cyclic diguanosine monophosphate (c-di-GMP), and small RNAs (sRNAs) are considered to be involved in the regulation of biofilm development and dispersion [20,21]. The QS is a special language used by bacteria for intercellular communication, which functions by small signal molecules called autoinducers [19].…”

Section: Biofilm Development and Molecular Biologymentioning

confidence: 99%

“…The c-di-GMPbased system regulates EPS synthesis, eDNA secretion, syntheses of pili and adhesins (a virulence factor) and controls cell death and motility [19]. Finally, sRNAs participate in a wide range of post-transcriptional gene regulation in bacteria [21,27,28]. Hence, sRNAs are considered to be involved in regulating the biofilm life cycle of bacteria, e.g., regulation of EPS synthesis, regulation of flagella, curli and cell surface structures as well as the regulation of biofilm-associated transcriptional and post-transcriptional regulators [21].…”

Section: Biofilm Development and Molecular Biologymentioning

confidence: 99%

Biofilms as Battlefield Armor for Bacteria against Antibiotics: Challenges and Combating Strategies

Bano,

Hassan,

Rafiq

et al. 2023

Microorganisms

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Bacterial biofilms are formed by communities, which are encased in a matrix of extracellular polymeric substances (EPS). Notably, bacteria in biofilms display a set of ‘emergent properties’ that vary considerably from free-living bacterial cells. Biofilms help bacteria to survive under multiple stressful conditions such as providing immunity against antibiotics. Apart from the provision of multi-layered defense for enabling poor antibiotic absorption and adaptive persistor cells, biofilms utilize their extracellular components, e.g., extracellular DNA (eDNA), chemical-like catalase, various genes and their regulators to combat antibiotics. The response of biofilms depends on the type of antibiotic that comes into contact with biofilms. For example, excessive production of eDNA exerts resistance against cell wall and DNA targeting antibiotics and the release of antagonist chemicals neutralizes cell membrane inhibitors, whereas the induction of protein and folic acid antibiotics inside cells is lowered by mutating genes and their regulators. Here, we review the current state of knowledge of biofilm-based resistance to various antibiotic classes in bacteria and genes responsible for biofilm development, and the key role of quorum sensing in developing biofilms and antibiotic resistance is also discussed. In this review, we also highlight new and modified techniques such as CRISPR/Cas, nanotechnology and bacteriophage therapy. These technologies might be useful to eliminate pathogens residing in biofilms by combating biofilm-induced antibiotic resistance and making this world free of antibiotic resistance.

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“…More recently, eRNA has also been shown to be a component of some biofilms, and like eDNA, contributes to overall biofilm structure including its distinctive viscoelastic properties (25). Further, several noncoding RNAs have been shown to regulate biofilm assembly by mediating curli production and exopolysaccharide synthesis (26). Polysaccharides, often mucopolysaccharides/glycosaminoglycans, are the major component of the EPS providing the “glue” important for the structure and adhesive properties of the biofilm.…”

Section: Introductionmentioning

confidence: 99%

The Mouse Epididymal Amyloid Matrix: A Mammalian Counterpart of a Bacterial Biofilm

Myers,

Atkins,

Villarreal

et al. 2023

Preprint

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The mouse epididymis is a long tubule connecting the testis to the vas deferens. Its primary functions are to mature spermatozoa into motile and fertile cells and to protect them from pathogens that ascend the male tract. We previously demonstrated that a functional extracellular amyloid matrix surrounds spermatozoa in the epididymal lumen and has host defense functions; properties not unlike that of an extracellular biofilm that surrounds and protects a bacterial community. Here we show the epididymal amyloid matrix also structurally resembles a biofilm by containing eDNA, eRNA, and mucin-like polysaccharides. Further these structural components exhibit comparable behaviors and perform functions like their counterparts in bacterial biofilms. Our studies suggest that nature has used the ancient building blocks of bacterial biofilms to form an analogous structure that nurtures and protects the mammalian male germline.

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Regulation of biofilm formation by non-coding RNA in prokaryotes

Cited by 7 publications

References 52 publications

Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection

Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection

Biofilms as Battlefield Armor for Bacteria against Antibiotics: Challenges and Combating Strategies

The Mouse Epididymal Amyloid Matrix: A Mammalian Counterpart of a Bacterial Biofilm

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