Regulation of c-myc protooncogene by antisense RNA.

Yokoyama, Kazushige; Fujita, Akiko; Ko, Kei; Nakata, Yasuhiko

doi:10.2745/dds.7.417

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“…We found that the antisense effect occurs during transcription via a specific transcriptional factor as described previously (13)(14)(15). We used preleukemic HL60 cells to which we introduced antisense c-myc gene b y protoplast fusion (13)(14)(15). We identified a new transcriptional factor, THZif formed HL60 cells (AM93-4-12) (13).…”

Section: Zinc Finger 1 55 H a C E M C G K A F R D V Y H L N R H K L Ssupporting

confidence: 67%

“…Herein, we further examine the characteristics of encoded protein of THZif-I. In a previous study, we purified DNA-binding proteins specific for the nuclease-hypersensitive element ( N H E = CACCTCC-repeated sequence) of the c-myc gene from a nuclear extract of antisense c-myc AM93-4-12 cells (13)(14)(15)(16)(17)(18)(19), and we isolated a recombinant cDNA clone (pTHZif-I) of the corresponding gene to examine the molecular basis of the DNA-protein complex between NHE and the DNA-binding protein. 'The purified protein (2.5 pg) was obtained and cleaved with TPCK-trypsin.…”

Section: Zinc Finger 1 55 H a C E M C G K A F R D V Y H L N R H K L Smentioning

confidence: 99%

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Specific Regulation of Gene Expression by Antisense Nucleic Acids: A Summary of Methodologies and Associated Problems

Kawasaki

Komatsu

et al. 1996

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Gene therapy based on gene-specific nucleic acids has moved from theory to a practical possibility in a very short time. The new DNA and RNA therapeutic reagents are intended to stop the growth of cancerous cells or the production of viruses. At the practical level, the efficacy of antisense oligomers as therapeutic reagents has been carefully examined in various clinical contexts. For the efficient use of antisense nucleic acids as pharmaceutical agents, a complete analysis of their mechanisms of action is necessary. The use of antisense oligomers always involves the following problems: basepair specificity, stereoisomer specificity, stability and resistance to nucleases of sense-antisense duplexes, permeability of the cell membrane and targeting of the oligomer, safety, and the preparation of large amounts of oligomer. Herein, we review the basic concepts and problems associated with the exploitation of antisense technology. We have identified a new transcription factor triple-helix-binding zinc-finger protein-1 (THZif-1) induced by antisense c-myc RNA in the antisense-transformed HL60 cells. The encoded protein functions as the repressor of c-myc to achieve the reduction of the endogenous expression of c-myc gene. Therefore, the introduction of THZif-1 gene into HL60 cells in conjunction with antisense c-myc oligomers may result in the efficient repression of the expression of the c-myc gene. The molecular features of this factor are herein discussed.

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Section: Zinc Finger 1 55 H a C E M C G K A F R D V Y H L N R H K L Ssupporting

confidence: 67%

Section: Zinc Finger 1 55 H a C E M C G K A F R D V Y H L N R H K L Smentioning

confidence: 99%

Specific Regulation of Gene Expression by Antisense Nucleic Acids: A Summary of Methodologies and Associated Problems

Kawasaki

Komatsu

et al. 1996

Artificial Organs

Self Cite

View full text Add to dashboard Cite

show abstract

Regulation of c-myc protooncogene by antisense RNA.

Cited by 1 publication

References 0 publications

Specific Regulation of Gene Expression by Antisense Nucleic Acids: A Summary of Methodologies and Associated Problems

Specific Regulation of Gene Expression by Antisense Nucleic Acids: A Summary of Methodologies and Associated Problems

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