Regulation of cadmium(2+)/zinc(2+)-stimulated metallothionein synthesis during induction, deinduction, and superinduction

Hildebrand, C.E.; Enger, M. Duane

doi:10.1021/bi00566a029

Cited by 38 publications

(15 citation statements)

References 48 publications

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“…1), the availability of a unique flowsorted chromosome-specific cosmid library 2 , and access to a mouse-human hybrid cell panel enabling the localization of clones to discrete cytogenetic intervals 3 . Further interest in human chromosome 16 stemmed from the clustering of metallothionein genes on this chromosome, which participate in heavy metal transport and detoxification, coinciding with important biological interests of the DOE 4,5 . Here we describe the finished human chromosome 16 sequence, which provides a reference for the further exploration of genomic sequence alterations and their relationship to human biology.…”

mentioning

confidence: 99%

The sequence and analysis of duplication-rich human chromosome 16

Martin

Han

Gordon

et al. 2004

Nature

156

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mentioning

confidence: 99%

The sequence and analysis of duplication-rich human chromosome 16

Martin

Han

Gordon

et al. 2004

Nature

156

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“…Studies on MT induction with cultured cells retaining the properties of the original organ would therefore be of great help to know tissue-specific regulation of MT synthesis. MTs or MTlike proteins have been reported in cultured cells derived from various organs, including liver (Hidalgo et al, 1978), kidney (Koizumi et al, 1982), skin (Rugstad and Norseth, 1975), ovary (Hildebrand and Enger, 1980) and uterus (Koizumi et al, 1985a). Nevertheless, information about the characteristics of the induction are restricted to only a few kinds of cell types, and it still remains obscure whether the responsiveness of the cells to the inducers reflects the characteristics of the organ they are derived from.…”

mentioning

confidence: 99%

Induction of metallothionein synthesis in cultured cells derived from rabbit kidney

Koizumi¹,

Sone²,

Kimura³

1985

Journal Cellular Physiology

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Metallothionein (MT) synthesis in rabbit kidney-derived RK-13 cells was studied. In response to Cd2+, RK-13 cells synthesized proteins closely similar in chromatographic and electrophoretic behaviors to the liver MTs induced in Cd2+-injected rabbit. These proteins were specifically immunoprecipitated by anti-mouse liver MT-II serum. The rate of RK-13 thionein (apoprotein of MT) synthesis rapidly increased after exposure to 1 microgram/ml of Cd2+, and reached the maximum in 7 h. The dose-response curve for the synthesis was biphasic; a sharp increase up to 0.5 microgram/ml and a slower increase at higher concentrations. RK-13 cells retained kidney-specific properties in terms of responsiveness of thionein synthesis to inducers; The MTs were inducible also by Zn2+ and probably by Hg2+, but not by dexamethasone. This system would therefore be a useful model in vitro for studying the regulation of MT synthesis in kidney cells.

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“…The concentrations of cadmium and zinc sulfate were slowly increased to 150 fiM, where cell proliferation was inhibited. The logic behind this is that cadmium can substitute for zinc in metalloenzymes; by simultaneously adding exogenous zinc, it is possible to maintain zinc homeostasis while the zinc further stresses the cells (Hildebrand and Enger, 1980). Using this protocol, cells were acclimated to growth in 150 ¡iM CdCl2/300 ¡iM ZnCl2, and are referred to as constitutive.…”

Section: Cell Culturementioning

confidence: 99%

Expression of the 31-kD Stress Protein in Rat Myoblasts and Hepatocytes

Shuman¹,

Przybyla²

1988

DNA

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Rat tissue cultures cells respond to stress by inducing the synthesis of about 20 proteins, including two low-molecular-weight species of about 31 kD and 27 kD. We have cloned a cDNA for the 31-kD protein. This protein is induced in myoblasts and hepatoma cells in response to a 43 degrees C heat shock, or exposure to sodium arsenite or cadmium chloride salts. Furthermore, this protein is superinduced in hepatoma cells conditioned to grow in cadmium and zinc salts when they are exposed to a standard sodium arsenite stress. Induction of the gene encoding the 31-kD protein has been characterized as follows: (i) Transcripts accumulate maximally with similar kinetics when myoblasts are induced with either heat shock or sodium arsenite; (ii) accumulation of transcripts decays to preinduction levels within 4 hr of a heat shock, but requires more than 8 days after sodium arsenite stress; (iii) basal levels of transcript are reduced when myoblasts are cultured in the presence of steroid hormones; and (iv) stress induction is virtually abolished once myoblasts have differentiated.

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Regulation of cadmium(2+)/zinc(2+)-stimulated metallothionein synthesis during induction, deinduction, and superinduction

Cited by 38 publications

References 48 publications

The sequence and analysis of duplication-rich human chromosome 16

The sequence and analysis of duplication-rich human chromosome 16

Induction of metallothionein synthesis in cultured cells derived from rabbit kidney

Expression of the 31-kD Stress Protein in Rat Myoblasts and Hepatocytes

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