Gene Manipulation and Expression 1985
DOI: 10.1007/978-94-011-6565-5_26
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Regulation of cAMP Synthesis in Streptomyces Granaticolor

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1985
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“…(i) Adenylate cyclase activity was found either in the pellet or in the supernatant fractions, depending on the methods used for cellFdisruption and fractionation. This activity was very low compared with the activity in intact cells or in toluene-treated cells (40,131,151,219,267), and decreased nonlinearly on dilution, perhaps indicating the dissociation of a protein complex (401). (ii) Only intact cells Qr cells permeabilized by toluene treatment retained the regulation of adenylate cyclase activity, as if a regulatory protein or subunit dissociates irreversibly on dilution (131).…”
Section: Role Of the Pts In Catabolite Repression And Inducer Exclusionmentioning
confidence: 99%
“…(i) Adenylate cyclase activity was found either in the pellet or in the supernatant fractions, depending on the methods used for cellFdisruption and fractionation. This activity was very low compared with the activity in intact cells or in toluene-treated cells (40,131,151,219,267), and decreased nonlinearly on dilution, perhaps indicating the dissociation of a protein complex (401). (ii) Only intact cells Qr cells permeabilized by toluene treatment retained the regulation of adenylate cyclase activity, as if a regulatory protein or subunit dissociates irreversibly on dilution (131).…”
Section: Role Of the Pts In Catabolite Repression And Inducer Exclusionmentioning
confidence: 99%