Regulation of cathepsin B and L expression in vitro in gastric cancer tissues by egg cystatin

Saleh, Yousif; Siewiński, Maciej; Kielan, Wojciech; Ziółkowski, Piotr; Gryboś, Marian; Rybka, Jacek

doi:10.1111/j.1533-869x.2003.01105.x

Cited by 22 publications

(17 citation statements)

References 28 publications

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“…Some of the minor proteins in egg white are important for their possible role as an extracellular matrix. For example, cystatin was one of the first cysteine peptidase inhibitors (CPIs) identified and may have an important role in cancer metastasis (14). In addition, cystatin C acts as a TGF- receptor antagonist and is capable of inhibiting TGF- signaling.…”

Section: Discussionmentioning

confidence: 99%

Novel Egg White–based 3-D Cell Culture System

et al. 2008

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Although three dimensional (3-D) cell culture systems have numerous advantages over traditional monolayer culture, the currently available 3-D cell culture media are cost-prohibitive for regular use by the majority of research laboratories. Here we show a simple system based on avian egg white that supports growth of cells in 3-D, at a significantly decreased cost. Specifically, we show that growth of immortalized human breast epithelial cells (MCF10A) in egg white-based medium results in formation of acini with hollow lumens, apoptotic clearance of the cells in the lumen, and apicobasal polarization comparable to what has been described using established 3-D culture media such as reconstituted basement membrane preparations (BM). There was no significant difference in MCF10A proliferation and acinar size between egg white and BM. We also cultured different established cell lines, oncogene-transformed MCF10A, and mouse mammary epithelial cells in egg white and BM, and observed similar morphology. In summary, our data convincingly argue that egg white can be used as a suitable alternative model for 3-D cell culture studies. We strongly believe that this simple and inexpensive method should allow researchers to perform 3-D cell culture experiments on a regular basis, and result in a dramatic increase of use of the 3-D cell culture in research. Thus, this finding lays the foundation for significantly increased, cost-effective use of 3-D cultures in cell biology.

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Section: Discussionmentioning

confidence: 99%

Novel Egg White–based 3-D Cell Culture System

et al. 2008

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“…The inhibitors have been found to control in vitro the activity of the enzymes in cancerous tissue homogenates in patients operated for malignant neoplasms of the digestive track [26].…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescence was measured at 370 nm excitation and 440 nm emission wavelengths using a Luminescence Spectrometer, Perkin Elmer LS 50 B. One mEu of inhibitor activity was defined as a quantity of cathepsin B releasing 1 nM of 7-AMC [14,17].…”

Section: Methodsmentioning

confidence: 99%

Inhibition of cysteine peptidase activity in ascitic fluid in pancreatic cancer patients.

Agrawal

Kielan²,

Katib³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:The work's objective is to answer the question whether there is any possibility of activity inhibition of cysteine peptidases inhibitors playing an important role in key processes accompanying cancer formation, including pancreas. There is a justified speculation that specific inhibitors of these enzymes may inhibit development of cancer processes by inhibiting their activity. In vitro studies confirmed that these enzymes in ascitic fluid were inhibited with egg whites inhibitors even to 90% of their original activity.

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“…This enzymes have been purposefully selected for this study since information obtained from the literature indicates that they play a key role in basic processes of initiation and development of malignancies, such as: malignant transformation, invasion, metastases and angiogenesis. These enzymes were found to play a significant role in processes that accompany apoptosis while abnormalities or apoptosis determine high stability of malignant cells and their resistance to current anti-cancer drugs (6,(13)(14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

Hap¹,

Kielan²,

Siewiński³

et al. 2010

Polish Journal of Surgery

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4Large intestine malignancy is the second most common malignancy and second leading cause of cancer mortality in Poland. This is related to late detection of these lesions, e.g. due to lack of effective screening tests. Lesions found by a surgeon are clinically advanced, making the treatment often ineffective and sometimes even completely impossible. Discovery of a substance that would be able to stop key processes for the development of malignancy could change such situation. Activity of certain enzymes was found to increase in malignant cells and invasion of malignancy could be triggered by inadequate amount of endogenous inhibitors of these enzymes in the surrounding healthy tissues. Inhibitors identical with that produced in human cells were found in egg whites. the aim of the study was to determine ability of cystatin isolated from egg whites to inhibit activity of cathepsin B and L. material and methods. Immunohistochemistry and histology of tissue specimen collected from malignant lesions resected from 60 patients diagnosed with large intestine adenocarcinoma, who underwent surgical treatment in 2 nd Department of General and Oncological Surgery, Medical University of Wrocław between 2007 and 2009. Results. Differences were fund between health tissues, margins and center of the malignant lesions with regard to amount and distribution of stained cathepsin B -cystatin complexes. The above mentioned inhibitors were able to inhibit 90% of primary activity of cathepsin B and L in malignant tissues. Conclusions. Cystatins obtained from egg whites could be used as substances supporting anti-cancer therapy in the future. Key words: malignancy, large intestine, cystatins, cathepsins, inhibitor therapyIncidence of large intestine malignancy is growing and it is estimated that a lifetime risk of large intestine malignancy among the worldwide population is 6%; high percentage of them will die. Cancer of the large intestine (colorectal cancer) is the second most common malignancy and second leading cause of cancer mortality in Poland, both in women and men. Its incidence in our country is increasing at a rate of 2.5% per year. Colorectal cancer is diagnosed too late in our country. Currently no screening method that would result in early detection of colorectal cancer with high sensitivity and specificity, that would be cheap and accepted by patients, is available. Studies and analyses suggest that the most effective screening programs are based on colonoscopy.

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Regulation of cathepsin B and L expression in vitro in gastric cancer tissues by egg cystatin

Abstract: Our experiment demonstrated that the cysteine peptidases cathepsins B and L may be useful for the early detection of gastric cancer. The results suggest that addition of egg white cystatin reduces the activities of cathepsins B and L to that of non-cancerous values.

Cited by 22 publications

References 28 publications

Novel Egg White–based 3-D Cell Culture System

Novel Egg White–based 3-D Cell Culture System

Inhibition of cysteine peptidase activity in ascitic fluid in pancreatic cancer patients.

Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

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