Regulation of CATSPER1 expression by the testis-determining gene SRY

Olivares, Aleida; Hernández-Reyes, Adriana; Felix, Ricardo; Forero, Ángela; Mata-Rocha, Minerva; Hernández‐Sánchez, Javier; Santos, Ivanaldo; Aguirre-Alvarado, Charmina; Oviedo, Norma

doi:10.1371/journal.pone.0205744

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…When the expression of the SOX gene family was downregulated, the flagellar structure changed, and spermatogenesis, morphology, and motility declined. Meanwhile, the SOX genes interacted with the promoter regions of sperm motility candidate genes such as CATSPER1 ( 42 ). The upregulation of the SOX genes may further promote the expression of CATSPER1 gene, thereby increasing sperm motility and fertility rate.…”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of differential dietary energy levels on spermatogenesis and sperm motility of yellow-feathered breeder cocks

Xue

Liu²,

Lv³

et al. 2022

Front. Vet. Sci.

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The semen quality of breeder cocks profoundly impacted the numbers of matched layer hens and the economic benefits of the poultry industry. Adequacy and balance of poultry nutrition, especially the energy provision, critically modulated the reproductive potential of breeder cocks, however, the underlying mechanism was still unclear. For the purpose of this study, a total of 90 yellow-feathered 13-week-old roosters with the same age in days and similar body weight (1,437 ± 44.3 g) were selected and randomly divided into the low energy diet (LE), the moderate energy diet (ME), and the high energy diet (HE) treatments. The phenotypic parameters related to reproduction include semen quality, fertility, and hatchability, and the testis morphological parameters, including seminiferous epithelium length (SEL), seminiferous tubule perimeter (STP), seminiferous tubule area (STA), and Johnsen score, were measured to investigate the regulatory effects of different energy diets on reproductive performances. Furthermore, spermatogenesis and sperm motility-related genes, which included the sry-related high mobility group box (SOX) gene family and sperm-associated antigen (SPAG) gene family, and mitochondria apoptosis-related genes, such as Cyt-C, Bcl-2, and Bax, were measured to determine the underlying mechanism of energy on the reproductive performances. The The results showed that the gonadosomatic index and sperm motility in the ME treatment significantly increased compared with the LE treatment. Chickens in the ME treatment showed a preferable performance of testis development, especially a significant increment of SEL and Johnsen Score, compared with the LE and HE treatments. Finally, spermatogenesis-related genes, which included SPAG6, SPAG16, SOX5, SOX6, and SOX13, and apoptosis-related genes of mitochondria, such as the Cyt-C and Bcl-2, were significantly upregulated in the ME treatment. This study concluded that proper energy provision stimulated regular energy metabolism for spermatogenesis and sperm capacitation, which finally increased semen quality and reproductive performances of breeder cocks.

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Section: Discussionmentioning

confidence: 99%

Regulatory effects of differential dietary energy levels on spermatogenesis and sperm motility of yellow-feathered breeder cocks

Xue

Liu²,

Lv³

et al. 2022

Front. Vet. Sci.

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“…The available evidence indicates that the dynamics of channel expression during spermatogenesis are highly complex and observations are overwhelmingly restricted to examples from mouse experiments. Transcriptional control regions and transcription factors that influence CATSPER1 promoter activity in vitro have been described (Mata-Rocha et al., 2013, 2014; Olivares et al., 2018; Oviedo et al., 2018). CatSper subunit gene expression in mice is developmentally regulated.…”

Section: Molecular and Functional Deficits Of Human Sperm With Channementioning

confidence: 99%

Human sperm ion channel (dys)function: implications for fertilization

Brown

Publicover

Barratt

et al. 2019

Human Reproduction Update

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BACKGROUNDIntensive research on sperm ion channels has identified members of several ion channel families in both mouse and human sperm. Gene knock-out studies have unequivocally demonstrated the importance of the calcium and potassium conductances in sperm for fertility. In both species, the calcium current is carried by the highly complex cation channel of sperm (CatSper). In mouse sperm, the potassium current has been conclusively shown to be carried by a channel consisting of the pore forming subunit SLO3 and auxiliary subunit leucine-rich repeat-containing 52 (LRRC52). However, in human sperm it is controversial whether the pore forming subunit of the channel is composed of SLO3 and/or SLO1. Deciphering the role of the proton-specific Hv1 channel is more challenging as it is only expressed in human sperm. However, definitive evidence for a role in, and importance for, human fertility can only be determined through studies using clinical samples.OBJECTIVE AND RATIONALEThis review aims to provide insight into the role of sperm ion channels in human fertilization as evidenced from recent studies of sperm from infertile men. We also summarize the key discoveries from mouse ion channel knock-out models and contrast the properties of mouse and human CatSper and potassium currents. We detail the evidence for, and consequences of, defective ion channels in human sperm and discuss hypotheses to explain how defects arise and why affected sperm have impaired fertilization potential.SEARCH METHODSRelevant studies were identified using PubMed and were limited to ion channels that have been characterized in mouse and human sperm. Additional notable examples from other species are included as appropriate.OUTCOMESThere are now well-documented fundamental differences between the properties of CatSper and potassium channel currents in mouse and human sperm. However, in both species, sperm lacking either channel cannot fertilize in vivo and CatSper-null sperm also fail to fertilize at IVF. Sperm-lacking potassium currents are capable of fertilizing at IVF, albeit at a much lower rate. However, additional complex and heterogeneous ion channel dysfunction has been reported in sperm from infertile men, the causes of which are unknown. Similarly, the nature of the functional impairment of affected patient sperm remains elusive. There are no reports of studies of Hv1 in human sperm from infertile men.WIDER IMPLICATIONSRecent studies using sperm from infertile men have given new insight and critical evidence supporting the supposition that calcium and potassium conductances are essential for human fertility. However, it should be highlighted that many fundamental questions remain regarding the nature of molecular and functional defects in sperm with dysfunctional ion channels. The development and application of advanced technologies remains a necessity to progress basic and clinical research in this area, with the aim of providing effective screening methodologies to identify and develop treatments for affected men in order t...

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“…The SRY gene, located on the Y chromosome in mammals, encodes the sex-determining region Y (SRY) protein, also known as testis-determining factor (TDF). This protein is one of the SOX (SRY-like box) transcription factors responsible for the development of gonadal cords, which subsequently differentiate into seminiferous tubules ( Olivares et al., 2018 ; Polanco & Koopman, 2007 ). Obviously, any mutation on coding or regulatory regions of the SRY gene can disrupt the development of the male genitals.…”

Section: Introductionmentioning

confidence: 99%

The study of a hermaphroditic sheep caused by a mutation in the promoter of SRY gene

Heidari,

Rahbaran,

Mirzaei

et al. 2023

Veterinary and Animal Science

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Regulation of CATSPER1 expression by the testis-determining gene SRY

Cited by 7 publications

References 28 publications

Regulatory effects of differential dietary energy levels on spermatogenesis and sperm motility of yellow-feathered breeder cocks

Regulatory effects of differential dietary energy levels on spermatogenesis and sperm motility of yellow-feathered breeder cocks

Human sperm ion channel (dys)function: implications for fertilization

The study of a hermaphroditic sheep caused by a mutation in the promoter of SRY gene

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