Regulation of Cell Fate in the Brain by GSK3

Cole, Adam R.

doi:10.5772/55180

Cited by 1 publication

(1 citation statement)

References 157 publications

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“…A pathway critical to intercellular communication is the Wnt/ β -catenin pathway (Baron et al 2006; de Lau et al 2007). Proper regulation of the Wnt signalling pathway is essential for normal brain development as it promotes proliferation and inhibits differentiation of pluripotent cells (Cole, 2013). The central component of the Wnt/ β -catenin signalling pathway is a protein complex assembled around the scaffold protein Axin.…”

Section: Introductionmentioning

confidence: 99%

Glycogen Synthase Kinase-3 is involved in glycogen metabolism control and embryogenesis of Rhodnius prolixus

et al. 2016

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Rhodnius prolixus is a blood-feeding insect that transmits Trypanosoma cruzi and Trypanosoma rangeli to vertebrate hosts. Rhodnius prolixus is also a classical model in insect physiology, and the recent availability of R. prolixus genome has opened new avenues on triatomine research. Glycogen synthase kinase 3 (GSK-3) is classically described as a key enzyme involved in glycogen metabolism, also acting as a downstream component of the Wnt pathway during embryogenesis. GSK-3 has been shown to be highly conserved among several organisms, mainly in the catalytic domain region. Meanwhile, the role of GSK-3 during R. prolixus embryogenesis or glycogen metabolism has not been investigated. Here we show that chemical inhibition of GSK-3 by alsterpaullone, an ATP-competitive inhibitor of GSK3, does not affect adult survival rate, though it alters oviposition and egg hatching. Specific GSK-3 gene silencing by dsRNA injection in adult females showed a similar phenotype. Furthermore, bright field and 4'-6-diamidino-2-phenylindole (DAPI) staining analysis revealed that ovaries and eggs from dsGSK-3 injected females exhibited specific morphological defects. We also demonstrate that glycogen content was inversely related to activity and transcription levels of GSK-3 during embryogenesis. Lastly, after GSK-3 knockdown, we observed changes in the expression of the Wingless (Wnt) downstream target β-catenin as well as in members of other pathways such as the receptor Notch. Taken together, our results show that GSK-3 regulation is essential for R. prolixus oogenesis and embryogenesis.

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Section: Introductionmentioning

confidence: 99%