Regulation of Cellular Response Pattern to Phosphorus Ion is a New Target for the Design of Tissue‐Engineered Blood Vessel

Chen, Wen; Wang, Fangjuan; Zeng, Wen; Sun, Jun; Li, Li; Yang, Mingcan; Sun, Jun; Wu, Yangxiao; Zhao, Xinshu; Zhu, Chuhong

doi:10.1002/adhm.201400763

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…Therefore, it is of great significance to the long‐term patency of TEBV through the target delivery of microenvironment optimizing molecules to EPCs while specifically capturing EPCs. Currently, the characterization method of one molecule with multiple functions is widely adopted, yet it still has a lot of deficiencies including limited effect and different concentration of molecules required for different functions . Based on exosomes and PEI/AuNP, one TEBV enabling the capturing of EPCs and the optimization of microenvironment was constructed through the self‐assembly mode.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, as a vesicular structure, exosomes did not produce any side effect resulted from the transplantation of MSCs, such as the differentiation of terminal cells. Klotho is an antiaging protein, which can inhibit the apoptosis, and the latest study reveals that klotho can capture the circulating EPCs . As the increase of klotho protein on the surface of exosome was induced by enhancing the expression of klotho protein in donor cells, the purpose of early endothelialization of TEBV could be achieved.…”

Section: Discussionmentioning

confidence: 99%

“…In the meantime, ADK inhibited cells can sustainably release adenosine which is an optimizing molecule for local microenvironment . EPC capture and antiapoptosis are functions of klotho …”

Section: Introductionmentioning

confidence: 99%

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Exosome‐Modified Tissue Engineered Blood Vessel for Endothelial Progenitor Cell Capture and Targeted siRNA Delivery

Chen

Yang

Bai

et al. 2017

Macromolecular Bioscience

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Instability and poor targeting causes the long-term patency of RNA-modified tissue engineering blood vessels (TEBVs) remaining unsatisfactory. RNA can be enriched in exosome and then delivered into targeted cells while whether exosome-modified TEBVs achieve RNA targeted delivery is unclear. Here, to promote the expression of klotho protein on the mesenchymal stem cell (MSC)-derived exosomes, klotho plasmids are first transfected into MSCs, and adenosine kinase (ADK) siRNA is then loaded into exosome (klotho/ADK siRNA-exosome) using electrotransfection. Flow chamber results show that klotho/ADK siRNA-exosome can effectively capture circulating endothelial progenitor cells (EPCs). Besides, the captured EPCs can endocytose this exosome, and then decompose it into klotho protein and ADK siRNA. Moreover, ADK siRNA promotes the paracrine of proangiogenic factors and adenosine from EPCs, which further facilitate proliferation and migration of endothelial cells. Based on polyethyleneimine-capped gold nanoparticles, exosome-modified TEBVs are constructed through layer-by-layer assembly. Animal experimental results show that klotho/ADK siRNA-exosome-modified TEBVs can maintain the patency up to one month, and good endothelialization is observed. In short, one exosome-modified TEBV is constructed, capture molecules on the surface of exosome capture the circulating EPCs, and the loaded RNA achieves its purpose of accurate treatment depending on the needs of patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Exosome‐Modified Tissue Engineered Blood Vessel for Endothelial Progenitor Cell Capture and Targeted siRNA Delivery

Chen

Yang

Bai

et al. 2017

Macromolecular Bioscience

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“…After TEBV transplantation, released extracellular nucleotides (ATP and ADP) will trigger inflammation and lead to an inflammatory microenvironment. Apyrase and 5′-NT hydrolyze these extracellular nucleotides into adenosine, thus altering an ATP/ADP-driven pro-inflammatory milieu to an adenosine-induced anti-inflammatory environment and accelerating the endothelialization of TEBVs. , Both in vitro and in vivo experiments have shown that the RGO-enzyme-coated TEBVs had good antiplatelet and antithrombotic functions. We believe that these easily prepared vessels can provide options for the preparation of clinically available TEBVs.…”

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confidence: 99%

Construction of Antithrombotic Tissue-Engineered Blood Vessel via Reduced Graphene Oxide Based Dual-Enzyme Biomimetic Cascade

Huo

Liu

et al. 2017

ACS Nano

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Thrombosis is one of the biggest obstacles in the clinical application of small-diameter tissue-engineered blood vessels (TEBVs). The implantation of an unmodified TEBV will lead to platelet aggregation and further activation of the coagulation cascade, in which the high concentration of adenosine diphosphate (ADP) that is released by platelets plays an important role. Inspired by the phenomenon that endothelial cells continuously generate endogenous antiplatelet substances via enzymatic reactions, we designed a reduced graphene oxide (RGO) based dual-enzyme biomimetic cascade to successively convert ADP into adenosine monophosphate (AMP) and AMP into adenosine. We used RGO as a support and bound apyrase and 5′-nucleotidase (5′-NT) on the surface of RGO through covalent bonds, and then, we modified the surface of the collagen-coated decellularized vascular matrix with the RGO-enzyme complexes, in which RGO functions as a platform with a large open surface area and minimal diffusion barriers for substrates/products to integrate two catalytic systems for cascading reactions. The experimental results demonstrate that the two enzymes can synergistically catalyze procoagulant ADP into anticoagulant AMP and adenosine successively under physiological conditions, thus reducing the concentration of ADP. AMP and adenosine can weaken or even reverse the platelet aggregation induced by ADP, thereby inhibiting thrombosis. Adenosine can also accelerate the endothelialization of TEBVs by regulating cellular energy metabolism and optimizing the microenvironment, thus ensuring the antithrombotic function and patency of TEBVs even after the RGO-enzyme complex loses its activity.

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“…Estudos in vitro demonstraram que a sobrecarga de P causa apoptose de células ósseas e endoteliais de maneira tempo e dose dependente (Meleti et al, 2000;Chen et al, 2015). Mansfield e cols.…”

Section: Fisiologia Do Intestino Delgadounclassified

Efeitos da sobrecarga de fósforo dietético na expressão dos cotransportadores NaP-IIb e PiT-1 em ratos controles e urêmicos

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Regulation of Cellular Response Pattern to Phosphorus Ion is a New Target for the Design of Tissue‐Engineered Blood Vessel

Cited by 8 publications

References 16 publications

Exosome‐Modified Tissue Engineered Blood Vessel for Endothelial Progenitor Cell Capture and Targeted siRNA Delivery

Exosome‐Modified Tissue Engineered Blood Vessel for Endothelial Progenitor Cell Capture and Targeted siRNA Delivery

Construction of Antithrombotic Tissue-Engineered Blood Vessel via Reduced Graphene Oxide Based Dual-Enzyme Biomimetic Cascade

Efeitos da sobrecarga de fósforo dietético na expressão dos cotransportadores NaP-IIb e PiT-1 em ratos controles e urêmicos

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