Regulation of chicken immunity-related genes and host response profiles against Avibacterium paragallinarum pathogen challenge

Boucher, Charlotte E.; Theron, Chrispian W.; Hitzeroth, Arina; Bragg, R.R.

doi:10.1016/j.vetimm.2015.06.005

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…After activation of Tlr4 , the adapter protein Myd88 was significantly upregulated in the nasal cavity and spleen at 1 dpi, indicating that the Tlr4 pathway was activated after A. paragallinarum challenge. Similarly, previous studies showed that the immune response was initiated by Tlr4 after infection with A. paragallinarum , which in turn led to the Th2 response in nasal tissue ( Boucher et al, 2014 , 2015 ). The expression of Nod1 was significantly upregulated in the spleen at 1 and 3 dpi.…”

Section: Discussionsupporting

confidence: 60%

Pathogenicity and innate response to Avibacterium paragallinarum in chickens

et al. 2022

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Infectious coryza ( IC ) is an acute infectious upper respiratory disease in chickens. Recently, the prevalence of IC has increased in China. In this study, to clarify the pathogenic mechanism and innate immune response of Avibacterium paragallinarum ( A. paragallinarum ), an infection experiment with A. paragallinarum was conducted. Our results showed that the whole course of IC was approximately 7 d. The clinical signs score was highest at 3 dpi and decreased from 5 dpi. A large amount of mucus and exudates was found in the infraorbital sinuses and nasal cavity. The A. paragallinarum contents in blood remained the highest, reaching 9.16 × 10 5 CFU/g at 5 dpi, which indicated that A. paragallinarum could rapidly invade the host, replicate in the blood and cause bacteremia. A. paragallinarum targets the upper respiratory tract. The infiltration of inflammatory cells, macrophages, and heterophilic granulocytes was only observed in the nasal cavity and infraorbital sinus. The Tlr4 and Nod1 pathways were activated and induced proinflammatory responses in chickens after infection with A. paragallinarum . The expression of Il1β and Il6 in the nasal cavity was significantly higher than that in the spleen, and it was consistent with the gross lesions and pathological changes. In particular, the expression of Il6 increased 229.07-fold at 1 dpi in the nasal cavity and increased 3.12-fold in the spleen. The high level of proinflammatory cytokines in the nasal cavity at an early stage of infection may be the main factor related to acute upper respiratory inflammation in chickens. These findings provide a reference for the occurrence and development of diseases mediated by A. paragallinarum .

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Section: Discussionsupporting

confidence: 60%

Pathogenicity and innate response to Avibacterium paragallinarum in chickens

et al. 2022

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“…A nasal challenge with Av. paragallinarum in chickens was previously shown to result in the increased local gene expression of IL-6, which may contribute to a Th17 adaptive immune response in mammals [49], whereas no changes were observed for the Th1-inducing cytokine IL-12p35 [50]. In this in vitro study, we found increased gene expression of the Th1/Th17-inducing cytokine IL-12p40.…”

Section: Discussioncontrasting

confidence: 39%

Macrophage Activation Assays to Evaluate the Immunostimulatory Capacity of Avibacterium paragallinarum in A Multivalent Poultry Vaccine

et al. 2020

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High-quality vaccines are crucial to prevent infectious disease outbreaks in the poultry industry. In vivo vaccination tests are routinely used to test poultry vaccines for their potency, i.e., their capacity to induce protection against the targeted diseases. A better understanding of how poultry vaccines activate immune cells will facilitate the replacement of in vivo potency tests for in vitro assays. Using the chicken macrophage-like HD11 cell line as a model to evaluate innate immune responses, the current explorative study addresses the immunostimulatory capacity of an inactivated multivalent vaccine for infectious bronchitis, Newcastle disease, egg-drop syndrome, and infectious coryza. The vaccine stimulated HD11 cells to produce nitric oxide and to express pro-inflammatory cytokines IL-1β, TNF, and IL-12p40, chemokines CXCLi1 and CXCLi2, and the anti-inflammatory cytokine IL-10, but only when inactivated Avibacterium paragallinarum, the causative agent of infectious coryza, was present. Lipopolysaccharides from Avibacterium paragallinarum were crucial for the production of nitric oxide and expression of IL-1β and CXCLi1. The described immune parameters demonstrate the capacity of this multivalent vaccine to activate innate immune cells and may in the future, combined with antigen quantification methods, contribute to vaccine quality testing in vitro, hence the replacement of current in vivo vaccination tests.

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“…The immunological response that is essential for defining the severity and physiological outcome of infectious diseases is poorly studied in infectious coryza. Available studies have evaluated only the local immune response by analysis of nasal tissue samples [ 9 , 10 ]. As a comprehensive overview of the immune system response is lacking, we evaluated the systemic effects of infection with Av.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the importance of the immune system in the progression of the disease, few studies have evaluated the role of cells or molecules associated with the immune response. Furthermore, although relevant information was obtained, the studies focused on the local immune response by analysis of nasal tissue samples [ 9 , 10 ]. However, the prognosis of a disease is a result of a more complicated interaction between the local and systemic immune responses.…”

Section: Introductionmentioning

confidence: 99%

A transient increase in MHC-IIlow monocytes after experimental infection with Avibacterium paragallinarum (serovar B-1) in SPF chickens

Alvarez

Poma-Acevedo

Fernández-Díaz

2020

Vet Res

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Infectious coryza (IC), an upper respiratory tract disease affecting chickens, is caused by Avibacterium paragallinarum. The clinical manifestations of IC include nasal discharge, facial swelling, and lacrimation. This acute disease results in high morbidity and low mortality, while the course of the disease is prolonged and mortality rates are increased in cases with secondary infections. Studies regarding the immune response in infected chickens are scarce, and the local immune response is the focal point of investigation. However, a large body of work has demonstrated that severe infections can impact the systemic immune response. The objective of this study was to evaluate the systemic effects of Avibacterium paragallinarum (serovar B-1) infection on immune cells in specific pathogen-free (SPF) chickens. The current study revealed the presence of a transient circulating monocyte population endowed with high phagocytic ability and clear downregulation of major histocompatibility complex class II (MHC-II) surface expression. In human and mouse studies, this monocyte population (identified as tolerant monocytes) has been correlated with a dysfunctional immune response, increasing the risk of secondary infections and mortality. Consistent with this dysfunctional immune response, we demonstrate that B cells from infected chickens produced fewer antibodies than those from control chickens. Moreover, T cells isolated from the peripheral blood of infected chickens had a lower ability to proliferate in response to concanavalin A than those isolated from control chickens. These findings could be related to the severe clinical signs observed in complicated IC caused by the presence of secondary infections.

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Regulation of chicken immunity-related genes and host response profiles against Avibacterium paragallinarum pathogen challenge

Cited by 3 publications

References 34 publications

Pathogenicity and innate response to Avibacterium paragallinarum in chickens

Pathogenicity and innate response to Avibacterium paragallinarum in chickens

Macrophage Activation Assays to Evaluate the Immunostimulatory Capacity of Avibacterium paragallinarum in A Multivalent Poultry Vaccine

A transient increase in MHC-IIlow monocytes after experimental infection with Avibacterium paragallinarum (serovar B-1) in SPF chickens

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