Regulation of chloroplast ultrastructure, adventitious bud anatomy and clonal fidelity of in vitro Zingiber officinale var. rubrum Theilade plantlets in response to LED spectral quality

Gnasekaran, Pavallekoodi; Rahman, Zuraida Abdul; Chew, Bee Lynn; Uddain, Jasim; Mariappan, Vanitha; Appalasamy, Suganthi; Subramaniam, Sreeramanan

doi:10.1016/j.indcrop.2022.114825

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…In the same way, the central vascular bundle of plants from Epidendrum fulgens grown with natural ventilation under combinations of white/high blue, deep red/white/medium blue, and deep red/white/far red/medium blue LED had a higher content of sclerenchyma [ 64 ]. Furthermore, Gnasekaran et al [ 65 ] found that LED spectral quality alters plant chloroplast ultrastructure through the effects on starch accumulation in Z. officinale . Summarizing, the effect of different LEDs sources at different concentrations in morpho-anatomic characteristics seems to depend on the species cultured at in vitro conditions.…”

Section: Discussionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

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Section: Discussionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Spectral lights influence growth and metabolic efficiency leading to enhanced phytochemical contents of Coriandrum sativum L.

Goswami,

Ballabh,

Debasmita

et al. 2024

Environmental and Experimental Botany

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Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

Rohela,

Saini

2023

Genome Size and Genetic Homogeneity of Regenerated Plants: Methods and Applications

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Most of the medicinal, aromatic and other commercially important crops are poor rooters, and some of them are sessile in terms of seed production; hence these plants are very difficult to propagate either through stem cuttings based vegetative propagation or through seedlings based sexual propagation. During the last two decades, plant tissue culture has emerged as an alternative technique for the propagation of plants with commercial importance. Majorly, the somatic tissues, viz., leaf, node and shoot tip, are being used as explants for the production of genetically similar plantlets through tissue culture studies. Recently, abnormalities with respect to ploidy level and genetic fidelity have been reported in In Vitro regenerated plantlets. This is mainly due to the usage of synthetic chemicals or artificial plant growth regulators in In Vitro culture studies, the fragile nature of callus and exposure of cultures to artificial light sources. In order to ensure the commercial production of genetically true clones of commercial plants, nowadays it has become an obligatory step to assess the ploidy level and genetic fidelity of regenerated plantlets with that of mother plants. This book chapter focussed on different molecular techniques which are in use for the detection of ploidy level and genetic fidelity of In Vitro micro propagated plantlets.

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Regulation of chloroplast ultrastructure, adventitious bud anatomy and clonal fidelity of in vitro Zingiber officinale var. rubrum Theilade plantlets in response to LED spectral quality

Cited by 3 publications

References 68 publications

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Spectral lights influence growth and metabolic efficiency leading to enhanced phytochemical contents of Coriandrum sativum L.

Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

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