Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2

Kadlecova, Zuzana; Spielman, Scott R.; Loerke, Dinah; Mohanakrishnan, Aparna; Reed, Dana Kim; Schmid, Sandra L.

doi:10.1083/jcb.201608071

Cited by 169 publications

(277 citation statements)

References 82 publications

(159 reference statements)

Supporting

Mentioning

264

Contrasting

Order By: Relevance

“…Here we use the term clathrin-labeled structures (CLSs) for clathrin structures observed or detected within single images (frames); identification of bona fide CCPs requires additional analysis of CLS behavior over time that is possible only when examining live-cell images (Aguet et al. , 2013; Kadlecova et al. , 2017).…”

Section: Resultsmentioning

confidence: 99%

“…The set of CLSs includes CCPs as well as other clathrin structures such as subthreshold CLSs (sCLSs), of which the latter do not give rise to bona fide CCPs (Aguet et al. , 2013; Mettlen and Danuser, 2014; Kadlecova et al. , 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, this method allows further resolution of each CLS as either a bona fide CCP or an sCLS, as previously described (Aguet et al. , 2013; Mettlen and Danuser, 2014; Kadlecova et al. , 2017).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Selective regulation of clathrin-mediated epidermal growth factor receptor signaling and endocytosis by phospholipase C and calcium

Santos

Bautista

Lucarelli

et al. 2017

MBoC

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Selective regulation of clathrin-mediated epidermal growth factor receptor signaling and endocytosis by phospholipase C and calcium

Santos

Bautista

Lucarelli

et al. 2017

MBoC

View full text Add to dashboard Cite

show abstract

“…Valid clathrin traces with lifetimes between 20 and 120 s and with significant sensor signal in the slave channel were automatically selected. The intensity-lifetime cohorts were generated as described 10 and showed similar distributions to those previously determined for endocytic clathrin-coated pits and vesicles imaged in htertRPE-1 and SUM159 cells 45,46 . The burst signal of the PtdIns4P sensor was calculated as the difference between maximum intensity during burst and average intensity from five early-stage frames starting 13 s before the peak.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of phosphoinositide conversion in clathrin-mediated endocytic traffic

Marsland

Upadhyayula

et al. 2017

Nature

137

135

View full text Add to dashboard Cite

Vesicular carriers transport proteins and lipids from one organelle to another, recognizing specific identifiers for the donor and acceptor membranes. Two important identifiers are phosphoinositides and GTP-bound GTPases, which provide well-defined but mutable labels. Phosphatidylinositol and its phosphorylated derivatives are present on the cytosolic faces of most cellular membranes1,2. Reversible phosphorylation of its headgroup produces seven distinct phosphoinositides. In endocytic traffic, phosphatidylinositol-4,5-biphosphate marks the plasma membrane, and phosphatidylinositol-3-phosphateand phosphatidylinositol-4-phosphate mark distinct endosomal compartments2,3. It is unknown what sequence of changes in lipid content confers on the vesicles their distinct identity at each intermediate step. Here we describe ‘coincidence-detecting’ sensors that selectively report the phosphoinositide composition of clathrin-associated structures, and the use of these sensors to follow the dynamics of phosphoinositide conversion during endocytosis. The membrane of an assembling coated pit, in equilibrium with the surrounding plasma membrane, contains phosphatidylinositol-4,5-biphosphate and a smaller amount of phosphatidylinositol-4-phosphate.Closure of the vesicle interrupts free exchange with the plasma membrane. A substantial burst of phosphatidylinositol-4-phosphate immediately after budding coincides with a burst of phosphatidylinositol-3-phosphate, distinct from any later encounter with the phosphatidylinositol-3-phosphate pool in early endosomes; phosphatidylinositol-3,4-biphosphate and the GTPase Rab5 then appear and remain as the uncoating vesicles mature into Rab5-positive endocytic intermediates. Our observations show that a cascade of molecular conversions, made possible by the separation of a vesicle from its parent membrane, can label membrane-traffic intermediates and determine their destinations.

show abstract

“…Meanwhile, actin filaments were known to be critical components in the formation of plasma-membrane invaginations and their scission, which played a prominent role in the internalization and transportation of macromolecules (Ben-Dov & Korenstein, 2013). Chloropromazine, which blocks the assembling of clathrin at the cell membrane, is typically used to inhibit the clathrin-mediated route (Kadlecova et al, 2016). Nystatin, which is a cholesterol sequestering agent, is known to function as an inhibitor of caveolae (or lipid raft) mediated endocytosis (Cartwright et al, 2012).…”

Section: Investigation Of Endocytosis Pathways In Caco-2 Cellsmentioning

confidence: 99%

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

et al. 2017

View full text Add to dashboard Cite

To investigate the absorption and delivery of ASP in gastrointestinal (GI) tract, cASP was successfully synthesized by chemically modifying with succinic anhydride and then conjugating with a near infrared fluorescent dye Cy5.5. Then, the capacity of oral absorption of cASP was evaluated. The results demonstrated that cASP had low toxicity and no disruption on the integrity of cell membrane. The endocytosis of cASP into the epithelial cells was time- and energy-dependent, which was mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes. Otherwise, the actin filaments played a relatively weak role at the same time. The transport study illustrated that cASP could penetrate through the epithelial monolayer and mainly mediated by the same routes as that in the endocytosis experiment. Moreover, both in vitro Ussing chamber and in vivo ligated intestinal loops models indicated that cASP could be diffused through the mucus barriers and be absorbed in the whole small intestine. Finally, near-infrared fluorescence imaging presented that cASP could be absorbed and circulated into the blood, then distributed into various organs after oral administration. In conclusion, ASP could be absorbed after oral administration through endocytosis process mainly mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes, then be absorbed and circulated into blood. This study presents a comprehensive understanding of oral delivery of cASP, which will provide theoretical basis for the clinical application of ASP.

show abstract

Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2

Cited by 169 publications

References 82 publications

Selective regulation of clathrin-mediated epidermal growth factor receptor signaling and endocytosis by phospholipase C and calcium

Selective regulation of clathrin-mediated epidermal growth factor receptor signaling and endocytosis by phospholipase C and calcium

Dynamics of phosphoinositide conversion in clathrin-mediated endocytic traffic

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

Contact Info

Product

Resources

About