Regulation of Compound Leaf Development by PHANTASTICA in Medicago truncatula

Ge, Liangfa; Jisheng, Peng; Berbel, Ana; Madueño, Francisco; Chen, Rujin

doi:10.1104/pp.113.229914

Cited by 43 publications

(60 citation statements)

References 55 publications

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“…Scanning electron microscopic imaging analysis of the shoot apical meristem was performed as previously described (31). Hitachi Tabletop Scanning Electron Microscope TM3000 was used for imaging epidermal cells.…”

Section: Methodsmentioning

confidence: 99%

“…RNA in situ hybridization was performed as previously described (31), with minor modifications (see SI Appendix for details).…”

Section: Methodsmentioning

confidence: 99%

“…The BS1-GFP fusion protein was either transiently expressed in tobacco leaves or stably expressed in transgenic Arabidopsis plants. The GFP signal was imaged, using the Leica SP2 laser confocal microscope as previously described (7,31).…”

Section: Methodsmentioning

confidence: 99%

“…Reverse transcription was performed using a Qiagen SuperScript II Kit (Qiagen). Quantitative PCR was conducted on a 7900HT Fast Real-Time PCR system (Applied Biosystems) as previously described (31).…”

Section: Methodsmentioning

confidence: 99%

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Increasing seed size and quality by manipulating BIG SEEDS1 in legume species

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

131

162

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Plant organs, such as seeds, are primary sources of food for both humans and animals. Seed size is one of the major agronomic traits that have been selected in crop plants during their domestication. Legume seeds are a major source of dietary proteins and oils. Here, we report a conserved role for the BIG SEEDS1 (BS1) gene in the control of seed size and weight in the model legume Medicago truncatula and the grain legume soybean (Glycine max). BS1 encodes a plant-specific transcription regulator and plays a key role in the control of the size of plant organs, including seeds, seed pods, and leaves, through a regulatory module that targets primary cell proliferation. Importantly, down-regulation of BS1 orthologs in soybean by an artificial microRNA significantly increased soybean seed size, weight, and amino acid content. Our results provide a strategy for the increase in yield and seed quality in legumes.plant organ size | seed size | forage quality | Medicago | soybean A s a key crop worldwide, soybean not only provides up to 69% of proteins and 30% of oils to the human diet (1) but also requires a low input of fertilizers due to its symbiotic nitrogen fixation ability (2), making it a highly valuable crop to secure global food supplies while contributing to sustainable agriculture. It is clear that the rapid increase of world population requires significant increases in crop production (3).Seed size is a major agronomic trait that has been selected in crop plants during their domestication (4-6). Due to whole genome duplication events that occurred ∼59 and ∼14 Mya (million years ago) (2), soybean has a complex genome structure. Thus, the isolation of key genes or quantitative trait loci (QTL) that control seed size and weight in soybean using conventional approaches can be a challenge and has not been reported to date. To overcome this challenge, we took a molecular genetics approach, using the legume plant Medicago truncatula as a genetic model. We identified BIG SEEDS1 (BS1) as a key gene that controls size of lateral organs, including seeds, seed pods, and leaves, in M. truncatula. Based on these results, we further identified two BS1 orthologs in soybean (Glycine max). Our objectives were to understand the role and regulatory mechanism of the BS1 gene in lateral organ size control in legume plants. Down-regulation of soybean BS1 genes using an artificial microRNA resulted in increased size of seeds, seed pods, and leaves, thus revealing a key and conserved role of BS1 in the control of organ size in legumes. Results and DiscussionIsolation and Characterization of M. truncatula big seeds1 Mutants.In a screen of the fast neutron bombardment (FNB)-induced deletion mutant collection of M. truncatula [cultivar (cv.) Jemalong A17] (7), a unique mutant with large seeds was isolated and named M. truncatula big seeds1-1 (mtbs1-1) (Fig. 1A). The mtbs1-1 mutant also exhibited larger seed pods and leaves than WT plants, suggesting a key role of BS1 in determining lateral organ size (SI Appendix, Figs. S1 and S2). Time...

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Section: Methodsmentioning

confidence: 99%

“…RNA in situ hybridization was performed as previously described (31), with minor modifications (see SI Appendix for details).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Increasing seed size and quality by manipulating BIG SEEDS1 in legume species

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

131

162

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“…PHAN has an important role in stabilizing and maintaining the leaf adaxial-abaxial polarity, proximodistal axis development in simple and compound leaves and also leaf margin development (Zoulias et al 2012;Kim et al 2003;Ge et al 2014). It was also found to be responsible for drought tolerance in tobacco through an ABA dependent mechanism (Huang et al 2013).…”

Section: Protein Involved In Organ Identity Determinationmentioning

confidence: 99%

Hippophae rhamnoides N-glycoproteome analysis: a small step towards sea buckthorn proteome mining

Sougrakpam

Deswal

2016

Physiol Mol Biol Plants

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Hippophae rhamnoides is a hardy shrub capable of growing under extreme environmental conditions namely, high salt, drought and cold. Its ability to grow under extreme conditions and its wide application in pharmaceutical and nutraceutical industry calls for its indepth analysis. N-glycoproteome mining by con A affinity chromatography from seedling was attempted. The glycoproteome was resolved on first and second dimension gel electrophoresis. A total of 48 spots were detected and 10 non-redundant proteins were identified by MALDI-TOF/ TOF. Arabidopsis thaliana protein disulfide isomerase-like 1-4 (ATPDIL1-4) electron transporter, protein disulphide isomerase, calreticulin 1 (CRT1), glycosyl hydrolase family 38 (GH 38) protein, phantastica, maturase k, Arabidopsis trithorax related protein 6 (ATXR 6), cysteine protease inhibitor were identified out of which ATXR 6, phantastica and putative ATPDIL1-4 electron transporter are novel glycoproteins. Calcium binding protein CRT1 was validated for its calcium binding by stains all staining. GO analysis showed involvement of GH 38 and ATXR 6 in glycan and lysine degradation pathways. This is to our knowledge the first report of glycoproteome analysis for any Elaeagnaceae member.

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Compound leaf development in Medicago truncatula

Chen

2019

The Model Legume Medicago Truncatula

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Regulation of Compound Leaf Development by PHANTASTICA in Medicago truncatula

Cited by 43 publications

References 55 publications

Increasing seed size and quality by manipulating BIG SEEDS1 in legume species

Increasing seed size and quality by manipulating BIG SEEDS1 in legume species

Hippophae rhamnoides N-glycoproteome analysis: a small step towards sea buckthorn proteome mining

Compound leaf development in Medicago truncatula

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