Regulation of defense responses via heat shock transcription factors in Cucumis sativus L. against Botrytis cinerea

Kharisma, Agung Dian; Arofatullah, Nur Akbar; Yamane, Kenji; Tanabata, Sayuri; Satô, Tatsuo

doi:10.1007/s10327-021-01041-6

Cited by 6 publications

(5 citation statements)

References 43 publications

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“…The HSFA4d subfamily has been explored somewhat in rice, where overexpression of OsHSFA4d exhibited increased heat tolerance in transgenic rice (Yamanouchi et al, 2002 ), and overexpression of the related wheat HSFA4a led to improved cadmium tolerance in transgenic rice (Shim et al, 2009 ). The HSFB2 subfamily has been observed as heat stress induced in pigeon pea ( Cajanus cajan ) and implicated in defense responses against botrytis in cucumber ( Cucumis sativus ) through induction of pathogenesis‐related defense proteins in response to heat stress (Kharisma et al, 2022 ; Ramakrishna et al, 2022 ). Maize HSFB2a was not differentially expressed in the 1‐h heat stress data used here, but a previous study suggested that it was transiently induced at 30‐min post heat stress before returning to its prestress expression level (Zhou et al, 2021 ), suggesting a potentially different temporal response between the two species.…”

Section: Discussionmentioning

confidence: 99%

Conserved and variable heat stress responses of the Heat Shock Factor transcription factor family in maize and Setaria viridis

et al. 2023

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The Heat Shock Factor (HSF) transcription factor family is a central and required component of plant heat stress responses and acquired thermotolerance. The HSF family has dramatically expanded in plant lineages, often including a repertoire of 20 or more genes. Here we assess and compare the composition, heat responsiveness, and chromatin profiles of the HSF families in maize and Setaria viridis ( Setaria ), two model C4 panicoid grasses. Both species encode a similar number of HSFs, and examples of both conserved and variable expression responses to a heat stress event were observed between the two species. Chromatin accessibility and genome‐wide DNA‐binding profiles were generated to assess the chromatin of HSF family members with distinct responses to heat stress. We observed significant variability for both chromatin accessibility and promoter occupancy within similarly regulated sets of HSFs between Setaria and maize, as well as between syntenic pairs of maize HSFs retained following its most recent genome duplication event. Additionally, we observed the widespread presence of TF binding at HSF promoters in control conditions, even at HSFs that are only expressed in response to heat stress. TF‐binding peaks were typically near putative HSF‐binding sites in HSFs upregulated in response to heat stress, but not in stable or not expressed HSFs. These observations collectively support a complex scenario of expansion and subfunctionalization within this transcription factor family and suggest that within‐family HSF transcriptional regulation is a conserved, defining feature of the family.

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Section: Discussionmentioning

confidence: 99%

Conserved and variable heat stress responses of the Heat Shock Factor transcription factor family in maize and Setaria viridis

et al. 2023

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“…(Yu et al, 2016), and many HSPs respond not only to abiotic stress, but also to pathogen infection (Bhattarai et al, 2007; Breiman, 2014). In recent years, HSPs in plants have attracted extensive attention due to their new functions in innate immunity (Arofatullah et al, 2019; Kharisma et al, 2022). In our study, the 12 h treatment induced up–regulation of HSP20 , HSP90 , and HSP17.4 in the infected plants, and the expression of HSP20 was mainly caused by environment–plant–pathogen interactions (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Regulating temperature and humidity inhibits the disease severity of tomato

Li,

2023

Preprint

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The environment significantly impacts the interactions between plants and pathogens, thus remarkably affecting crop disease occurrence. Regulating the environmental conditions in greenhouses to inhibit plant diseases is conducive to the development of green ecological agriculture. However, how to specific regulate the temperature and humidity to reduce plant diseases remain unclear. In this study, different methods of temperature and humidity regulation were performed. Histological observation and dual RNA-seq analysis showed that increasing temperature and decreasing humidity at 24 h post inoculation (hpi) for 12 h could effectively inhibit disease severity of tomatoes. The regulation induced the expression of heat shock proteins (HSPs) and defense-related genes of tomatoes in response to B. cinerea. Bcl-2-associated athanogenes (BAGs) may be involved in tomato resistance to B. cinerea mediated by temperature and humidity regulation. In addition, the infection process and the expression of toxin-related genes of B. cinerea such as sesquiterpene botrydial (BOT) and polyketide botcinic acid (BOA) was inhibited. Overall, we obtained the optimal regulation method of temperature and humidity to alleviate tomato gray mold, and preliminarily explored its mechanisms of inhibiting the disease.

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“…The HSFA4d subfamily has been explored somewhat in rice, where overexpression of OsHSFA4d exhibited increased heat tolerance in transgenic rice (Yamanouchi et al ., 2002), and overexpression of the related wheat HSFA4a led to improved Cadmium tolerance in transgenic rice (Shim et al ., 2009). The HSFB2 subfamily has been observed as heat stress induced in pigeonpea ( Cajanus cajan ) and implicated in defense responses against Botrytis in cucumber ( Cucumis sativus ) through induction of pathogenesis-related defense proteins in response to heat stress (Kharisma et al .,2022; Ramakrishna et al ., 2022). Maize HSFB2a was stably expressed in the one hour heat stress data used here, but a previous study suggested that it was transiently induced at thirty minutes post heat stress before returning to its pre-stress expression level (Zhou et al ., 2021), suggesting a potentially different temporal response between the two species.…”

Section: Discussionmentioning

confidence: 99%

Conserved and variable responses of the HEAT SHOCK FACTOR transcription factor family in maize and Setaria viridis

Myers

Wootan

Liang

et al. 2022

Preprint

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Responding to the environment is a core aspect of plant growth and development. Mounting an effective response is important for plants to balance growth and survival. The HEAT SHOCK FACTOR (HSF) transcription factor family is a central and required component of plant heat stress responses and acquired thermotolerance. The HSF family has dramatically expanded in plant lineages, often including a repertoire of 20 or more genes. Here we assess the composition and heat responsiveness of the HSF family in Setaria viridis (Setaria), a model C4 panicoid grass, and make targeted comparisons between the HSF families of Setaria and maize. Examples of both conserved and variable expression responses to a heat stress event were observed when comparing the two species. Novel and existing data on chromatin accessibility, histone modifications, and genome-wide DNA binding profiles were utilized to assess the chromatin of HSF family members with distinct responses to heat stress. We observed significant variability for both expression and chromatin state within syntenic and orthologous sets of HSFs between Setaria and maize, as well as between syntenic pairs of maize HSFs retained following its most recent genome duplication event. These observations collectively support a complex scenario of expansion and sub-functionalization within this transcription factor family that has significant untapped potential for better understanding the evolution of large gene families.Significance StatementA comparison of the Heat Shock Factor transcription factors in maize and Setaria reveals examples of consistent and variable expression responses to heat stress and provides insights into the role of chromatin in predicting expression responses.

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Regulation of defense responses via heat shock transcription factors in Cucumis sativus L. against Botrytis cinerea

Cited by 6 publications

References 43 publications

Conserved and variable heat stress responses of the Heat Shock Factor transcription factor family in maize and Setaria viridis

Conserved and variable heat stress responses of the Heat Shock Factor transcription factor family in maize and Setaria viridis

Regulating temperature and humidity inhibits the disease severity of tomato

Conserved and variable responses of the HEAT SHOCK FACTOR transcription factor family in maize and Setaria viridis

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