Regulation of Dictyostelium Early Development Genes in Signal Transduction Mutants

Wu, Lijia; Hansen, Dane R.; Franke, Jakob; Kessin, Richard H.; Podgorski, Gregory J.

doi:10.1006/dbio.1995.1267

Cited by 37 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generation of a PDE mutant pointed out the essential role of PDE in slug morphogenesis. Its role is also supported by results obtained from experiments in which phosphodiesterase inhibitor expression from a prestalk-specific promoter inhibited PDE activity and blocked slug morphogenesis (51).…”

Section: Discussionmentioning

confidence: 68%

Filamin-regulated F-actin Assembly Is Essential for Morphogenesis and Controls Phototaxis in Dictyostelium

Khaire

Müller

Blau-Wasser

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

Dictyostelium strains lacking the F-actin cross-linking protein filamin (ddFLN) have a severe phototaxis defect at the multicellular slug stage. Filamins are rod-shaped homodimers that cross-link the actin cytoskeleton into highly viscous, orthogonal networks. Each monomer chain of filamin is comprised of an F-actin-binding domain and a rod domain. In rescue experiments only intact filamin re-established correct phototaxis in filamin minus mutants, whereas C-terminally truncated filamin proteins that had lost the dimerization domain and molecules lacking internal repeats but retaining the dimerization domain did not rescue the phototaxis defect. Deletion of individual rod repeats also changed their subcellular localization, and mutant filamins in general were less enriched at the cell cortex as compared with the full-length protein and were increasingly present in the cytoplasm. For correct phototaxis ddFLN is only required at the tip of the slug because expression under control of the cell type-specific extracellular-matrix protein A (ecmA) promoter and mixing experiments with wild type cells supported phototactic orientation. Likewise, in chimeric slugs wild type cells were primarily found at the tip of the slug, which acts as an organizer in Dictyostelium morphogenesis.

show abstract

Section: Discussionmentioning

confidence: 68%

Filamin-regulated F-actin Assembly Is Essential for Morphogenesis and Controls Phototaxis in Dictyostelium

Khaire

Müller

Blau-Wasser

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Deletion of the gene coding for the regulatory subunit of PKA, which releases the catalytic subunit from inhibition, also causes development to proceed very rapidly (Abe and Yanagisawa, 1983;Simon et al, 1992). By contrast, deletion of the catalytic subunit of the PKA leads to an aggregateless phenotype (Mann et al, 1997;Wu et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

A novel partner forDictyosteliumfilamin is an α-helical developmentally regulated protein

Knuth

Khaire

Kuspa

et al. 2004

Journal of Cell Science

View full text Add to dashboard Cite

The filamins are a family of highly homologous actincrosslinking proteins that stabilize three-dimensional actin networks, link them to membrane proteins and direct intracellular signaling reactions to the actin scaffold through interaction with various binding partners. Here, we describe the first Dictyostelium filamin-interacting protein to be isolated - FIP, a 229.8 kDa protein with two α-helical coiled coil domains. FIP was identified in a yeast two-hybrid screen using the rod domain of filamin as bait. FIP can also be coimmunoprecipitated with filamin from cellular extracts. Deletion analysis located the interaction domain of FIP to a C-terminal region; by contrast, in filamin rods, repeats 2-4 interacted with the recombinant FIP protein. The 7 kb transcript of FIP is upregulated during early development. Monoclonal antibodies raised against a bacterially expressed FIP polypeptide recognize a 230 kDa developmentally regulated protein in western blots. Immunofluorescence analysis shows a punctate staining pattern in the cytosol and, in cell fractionation experiments, FIP is mainly found in the cytosolic fraction. A fusion protein composed of GFP and the C-terminal part localizes to the plasma membrane and is associated with the cytoskeleton. Expression of the fusion protein affects development and influences the size of the multicellular aggregates and the phototactic behavior of slugs. Thus, FIP might provide a candidate link between the dynamic actin cytoskeleton and signal transduction events during the multicellular stages of Dictyostelium amoebae.

show abstract

“…Aliquots of 2 x 107 cells were removed every 2 h from HPS400 and ga3-cultures developing in shaking culture as described in Wu et al (1995a). Some cultures were treated with 50 nM cAMP added every 6 min to mimic endogenous pulses of cAMP.…”

Section: Rna Preparation and Analysismentioning

confidence: 99%

G alpha 3 regulates the cAMP signaling system in Dictyostelium.

Brandon

Podgorski

1997

MBoC

View full text Add to dashboard Cite

The Dictyostelium discoideum developmental program is initiated by starvation and its progress depends on G-protein-regulated transmembrane signaling. Disruption of the Dictyostelium G-protein a-subunit Ga3 (ga3-) blocks development unless the mutant is starved in the presence of artificial cAMP pulses. The function of Ga3 was investigated by examining the expression of several components of the cAMP transmembrane signaling system in the ga3-mutant. cAMP receptor 1 protein, cyclic nucleotide phosphodiesterase, phosphodiesterase inhibitor, and aggregation-stage adenylyl cyclase mRNA expression were absent or greatly reduced when cells were starved without exogenously applied pulses of cAMP. However, cAMP receptor 1 protein and aggregation-stage adenylyl cyclase mRNA expression were restored by starving the ga3-cells in the presence of exogenous cAMP pulses. Adenylyl cyclase activity was also reduced in ga3-cells starved without exogenous cAMP pulses compared with similarly treated wild-type cells but was elevated to a level twofold greater than wild-type cells in ga3-cells starved in the presence of exogenous cAMP pulses. These results suggest that Ga3 is essential in early development because it controls the expression of components of the transmembrane signaling system.

show abstract

Regulation of Dictyostelium Early Development Genes in Signal Transduction Mutants

Cited by 37 publications

References 0 publications

Filamin-regulated F-actin Assembly Is Essential for Morphogenesis and Controls Phototaxis in Dictyostelium

Filamin-regulated F-actin Assembly Is Essential for Morphogenesis and Controls Phototaxis in Dictyostelium

A novel partner forDictyosteliumfilamin is an α-helical developmentally regulated protein

G alpha 3 regulates the cAMP signaling system in Dictyostelium.

Contact Info

Product

Resources

About