Regulation of differentiated properties and proliferation of arterial smooth muscle cells.

Proliferation and differentiation of smooth muscle cell precursors occurs simultaneously during the development of the vessel wall

“…Several groups have shown that in primary culture, proliferation of adult smooth muscle cells is followed by the loss of myosin and actin containing filaments (Chamley-Campbell et al, 1979;Thyberg et al, 1990). However, other experiments have indicated that the differentiation of SMCs in vitro is not totally dependent on a withdrawal from a proliferative state (Owens et al, 1986;De Mey et al, 1989;Owens, 1995).…”

“…Studies performed in vitro have demonstrated that the highly proliferative and synthetic SMC phenotype is associated with the loss of contractile proteins such as myosin and tropomyosin, and with some loss of SM a-actin (Chamley- Campbell et al, 1979;Thyberg et al, 1990;Owens, 1995). Recently, Belknap and colleagues (1996) showed that a rapid increase of tropoelastin expression, in the late fetal and early postnatal period, was associated with a decrease in SMC proliferation during rat aortic development.…”

Impact of Insulin-like Growth Factor-I on Migration, Proliferation and Akt-ERK Signaling in Early and Late-passages of Vascular Smooth Muscle Cells

“…Among these alterations, migration and proliferation of vascular smooth muscle cells (VSMCs) are critical for the onset and progression of atherosclerosis, a common problem with increased age [4][5][6]. VSMCs have been shown to shift from a pro-contractile phenotype into a synthetic/proliferative phenotype after an extended period in culture [7,8]. This phenotypic transition is reminiscent of in vivo neointima of a progressing atherosclerotic plaque following vascular injury [8].…”

Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

“…[5,61 Thus, SMC undergo the change of their phenotype and become synthetic and proliferative (SMCs). [7] [12,13] Our study was carried out on aortic SMCs Ross,[17] modified by Bourdillon et al [18] Figure 1 8,6 by the method of Towbin et al [22] Transferred proteins were incubated with first antiserum (at 4/100 dilution). After an overnight incubation at 4C, a second specific antiserum (at 1/250 dilution, Sanofy) linked to the peroxidase was added to bind the antigenantibody complex during a 2-h period at room temperature; the bands were revealed by tetrachloride 3-3 DAB 0.5%.…”