Regulation of drought tolerance by gene manipulation of 9‐<i>cis</i>‐epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in <i>Arabidopsis</i>

Iuchi, Satoshi; Kobayashi, Masatomo; Taji, Teruaki; Naramoto, Masaaki; Seki, Motoaki; Kato, Tomohiko; Tabata, Satoshi; Kakubari, Yoshitaka; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo

doi:10.1046/j.1365-313x.2001.01096.x

Cited by 1,170 publications

(1,001 citation statements)

References 39 publications

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“…Lastly, osmotic stress-induced starch degradation was abolished in the nced3 and aba2 mutants, but not in a mutant lacking AAO3 (Figures 6A and 6B; Supplemental Figure 10). Given that the major difference between these ABA biosynthetic mutants is their capacity to accumulate ABA in response to stress (Seo et al, 2000;Iuchi et al, 2001;Urano et al, 2009), our data suggest that the lack of stressinduced starch degradation in nced3 and aba2 was specifically caused by the complete absence of ABA accumulation. This is supported by the observation that the stress-induced transcriptional induction of BAM1 and AMY3 was substantially impaired in nced3, but not in aao3 ( Figure 6C).…”

Section: Aba Is the Primary Signal For Starch Degradation In Leaves Imentioning

confidence: 81%

“…The ABA-Deficient Mutants nced3 and aba2 Mimic the amy3 bam1 Phenotype under Osmotic Stress An essential step in stress-induced de novo ABA biosynthesis is the cleavage of epoxycarotenoids to produce xanthoxin (the first C15 intermediate) by the 9-cis EPOXYCAROTENOID DIOXYGE-NASE3 (NCED3) (Iuchi et al, 2001). This explains why the nced3-null mutant does not accumulate ABA in response to dehydration (Iuchi et al, 2001;Urano et al, 2009).…”

Section: Carbon Export To the Root And Osmolyte Accumulation During Omentioning

confidence: 99%

“…This explains why the nced3-null mutant does not accumulate ABA in response to dehydration (Iuchi et al, 2001;Urano et al, 2009). We used the nced3 mutant to further investigate the role of ABA in stress-responsive starch metabolism.…”

Section: Carbon Export To the Root And Osmolyte Accumulation During Omentioning

confidence: 99%

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Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

et al. 2016

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Starch serves functions that range over a timescale of minutes to years, according to the cell type from which it is derived. In guard cells, starch is rapidly mobilized by the synergistic action of b-AMYLASE1 (BAM1) and a-AMYLASE3 (AMY3) to promote stomatal opening. In the leaves, starch typically accumulates gradually during the day and is degraded at night by BAM3 to support heterotrophic metabolism. During osmotic stress, starch is degraded in the light by stress-activated BAM1 to release sugar and sugar-derived osmolytes. Here, we report that AMY3 is also involved in stress-induced starch degradation. Recently isolated Arabidopsis thaliana amy3 bam1 double mutants are hypersensitive to osmotic stress, showing impaired root growth. amy3 bam1 plants close their stomata under osmotic stress at similar rates as the wild type but fail to mobilize starch in the leaves. 14 C labeling showed that amy3 bam1 plants have reduced carbon export to the root, affecting osmolyte accumulation and root growth during stress. Using genetic approaches, we further demonstrate that abscisic acid controls the activity of BAM1 and AMY3 in leaves under osmotic stress through the AREB/ABF-SnRK2 kinase-signaling pathway. We propose that differential regulation and isoform subfunctionalization define starch-adaptive plasticity, ensuring an optimal carbon supply for continued growth under an ever-changing environment.

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Section: Aba Is the Primary Signal For Starch Degradation In Leaves Imentioning

confidence: 81%

Section: Carbon Export To the Root And Osmolyte Accumulation During Omentioning

confidence: 99%

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Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

et al. 2016

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“…The cloning and characterization of maize Viviparous14, which encodes NCED1, led to identification of NCED as a rate-controlling enzyme in stress-induced de novo biosynthesis of ABA, particularly in leaf tissue Tan et al, 1997). Similar results were obtained from characterization of other ortholo-gous genes, PvNCED1 of bean (Phaseolus vulgaris; Qin and Zeevaart, 1999), VuNCED1 of cowpea (Vigna unguiculata; Iuchi et al, 2000), LeNCED1 of tomato (Solanum lycopersicum; Thompson et al, 2000a), and AtNCED3 of Arabidopsis (Arabidopsis thaliana; Iuchi et al, 2001). Stress-induced elevation of NCED transcript levels was observed to precede ABA accumulation (Qin and Zeevaart, 1999), and up-or down-regulation of the gene affected both ABA levels and drought sensitivity (Thompson et al, 2000b;Iuchi et al, 2001).…”

mentioning

confidence: 74%

PSY3, a New Member of the Phytoene Synthase Gene Family Conserved in the Poaceae and Regulator of Abiotic Stress-Induced Root Carotenogenesis

Vallabhaneni²,

Wurtzel³

2007

Plant Physiology

242

216

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Abscisic acid (ABA) plays a vital role in mediating abiotic stress responses in plants. De novo ABA biosynthesis involves cleavage of carotenoid precursors by 9-cis-epoxycarotenoid dioxygenase (NCED), which is rate controlling in leaves and roots; however, additional bottlenecks in roots must be overcome, such as biosynthesis of upstream carotenoid precursors. Phytoene synthase (PSY) mediates the first committed step in carotenoid biosynthesis; with PSY3 described here, maize (Zea mays) and other members of the Poaceae have three paralogous genes, in contrast to only one in Arabidopsis thaliana. PSY gene duplication has led to subfunctionalization, with each paralog exhibiting differential gene expression. We showed that PSY3 encodes a functional enzyme for which maize transcript levels are regulated in response to abiotic stresses, drought, salt, and ABA. Drought-stressed roots showed elevated PSY3 transcripts and ABA, responses reversed by rehydration. By blocking root carotenoid biosynthesis with the maize y9 mutation, we demonstrated that PSY3 mRNA elevation correlates with carotenoid accumulation and that blocking carotenoid biosynthesis interferes with stress-induced ABA accumulation. In parallel, we observed elevated NCED transcripts and showed that, in contrast to dicots, root zeaxanthin epoxidase transcripts were unchanged. PSY3 was the only paralog for which transcripts were induced in roots and abiotic stress also affected leaf PSY2 transcript levels; PSY1 mRNA was not elevated in any tissues tested. Our results suggest that PSY3 expression influences root carotenogenesis and defines a potential bottleneck upstream of NCED; further examination of PSY3 in the grasses is of value for better understanding root-specific stress responses that impact plant yield.

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“…Thus, it has been demonstrated that transgenic plants producing high levels of ABA display improved growth under drought stress than wild type (Iuchi et al, 2001;Qin and Zeevaart, 2002). Priming of ABA biosynthesis can be obtained by direct overexpression of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in the biosynthetic pathway (Iuchi et al, 2001;Qin and Zeevaart, 2002), or through the use of chemicals that accelerate ABA accumulation (Jakab et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

et al. 2006

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Abscisic acid (ABA) plays a key role in plant responses to abiotic stress, particularly drought stress. A wide number of ABAhypersensitive mutants is known, however, only a few of them resist/avoid drought stress. In this work we have generated ABA-hypersensitive drought-avoidant mutants by simultaneous inactivation of two negative regulators of ABA signaling, i.e. the protein phosphatases type 2C (PP2Cs) ABA-INSENSITIVE1 (ABI1) and HYPERSENSITIVE TO ABA1 (HAB1). Two new recessive loss-of-function alleles of ABI1, abi1-2 and abi1-3, were identified in an Arabidopsis (Arabidopsis thaliana) T-DNA collection. These mutants showed enhanced responses to ABA both in seed and vegetative tissues, but only a limited effect on plant drought avoidance. In contrast, generation of double hab1-1 abi1-2 and hab1-1 abi1-3 mutants strongly increased plant responsiveness to ABA. Thus, both hab1-1 abi1-2 and hab1-1 abi1-3 were particularly sensitive to ABA-mediated inhibition of seed germination. Additionally, vegetative responses to ABA were reinforced in the double mutants, which showed a strong hypersensitivity to ABA in growth assays, stomatal closure, and induction of ABA-responsive genes. Transpirational water loss under drought conditions was noticeably reduced in the double mutants as compared to single parental mutants, which resulted in reduced water consumption of whole plants. Taken together, these results reveal cooperative negative regulation of ABA signaling by ABI1 and HAB1 and suggest that fine tuning of ABA signaling can be attained through combined action of PP2Cs. Finally, these results suggest that combined inactivation of specific PP2Cs involved in ABA signaling could provide an approach for improving crop performance under drought stress conditions.

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Regulation of drought tolerance by gene manipulation of 9‐cis‐epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis

Cited by 1,170 publications

References 39 publications

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

PSY3, a New Member of the Phytoene Synthase Gene Family Conserved in the Poaceae and Regulator of Abiotic Stress-Induced Root Carotenogenesis

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

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