Regulation of endothelial cell phenotype by biomimetic matrix coated on biomaterials for cardiovascular tissue engineering

Prasad, C. Krishna; Krishnan, Lissy K.

doi:10.1016/j.actbio.2007.05.012

Cited by 27 publications

(23 citation statements)

References 28 publications

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“…Demand for vascular grafts in modern medicine and surgery is vast and huge, and synthetic vascular grafts should have biocompatibility, anti-thrombosis, anti-infective, and living body without toxicity and/or carcinogenicity, etc [1,2]. However, in some cases, synthetic vascular grafts are lack of cell growth for biocompatibility [1,2].…”

Section: Introductionmentioning

confidence: 99%

Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

et al. 2013

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Section: Introductionmentioning

confidence: 99%

Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

et al. 2013

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“…In the case of in vitro endothelialization, the ECM could effectively attenuate the shedding of seeded endothelial cells (ECs) in the bloodstream by shear stress, 1 help to maintain their physiological phenotype, 2,3 and even prevent them from undergoing apoptosis. 4 In terms of in vivo endothelialization, the ECM was essential for cell adhesion and migration via integrin receptors.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Yang

Zhu

et al. 2013

Tissue Engineering Part A

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Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.

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“…In the study of Prasad & Krishnan, when ECs are grown on bare Ti material, they lose their normal phenotype and become prothrombotic in vitro [32]. In our previous study, two kinds of materials, Ti-O filmcoated stainless steel (Ti-O) and stainless steel (SS), were implanted in canine femoral arteries for five months.…”

Section: The Influence Of Metallic or Inorganic Substrate On Endothelmentioning

confidence: 99%

“…Several in vitro studies have indicated that most of the metallic materials do not support cell growth owing to the lack of biorecognition and/ or specific interaction [31,32]. When ECs are grown on bare material, they lose their normal phenotype and become more prothrombotic than they should be to prevent any thrombotic tendency of the device [32]. Secondly, endothelialization in vitro has two surgical procedures-one for obtaining the cells, and the other for implantation.…”

Section: Inducing Endothelialization In Vivomentioning

confidence: 99%

“…ECM not only plays a mechanical role in supporting and maintaining tissue architecture but also acts as a dynamic meshwork in actively regulating critical cellular functions such as migration, survival, proliferation and differentiation [29,30]. Several in vitro studies have indicated that most of the metallic materials do not support cell growth owing to the lack of biorecognition and/ or specific interaction [31,32]. When ECs are grown on bare material, they lose their normal phenotype and become more prothrombotic than they should be to prevent any thrombotic tendency of the device [32].…”

Section: Inducing Endothelialization In Vivomentioning

confidence: 99%

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Biomimetic modification of metallic cardiovascular biomaterials: from function mimicking to endothelialization in vivo

Weng

Chen

et al. 2012

Interface Focus.

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Biosystem–surface interactions play an important role in various biological events and determine the ultimate functionality of implanted devices. Endothelialization or mimicking of endothelium on the surface of cardiovascular materials is a promising way to solve the problems of material-induced thrombosis and restenosis. Meanwhile, a multifunctional surface design is needed as antithrombotic properties should be considered in the period when the implants are not yet completely endothelialized. In this article, we summarize some successful approaches used in our laboratory for constructing multifunctional endothelium-like surfaces on metallic cardiovascular biomaterials through chemical modification of the surface or by the introduction of specific biological molecules to induce self-endothelialization in vivo . Some directions on future research in these areas are also presented.

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Regulation of endothelial cell phenotype by biomimetic matrix coated on biomaterials for cardiovascular tissue engineering

Cited by 27 publications

References 28 publications

Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Biomimetic modification of metallic cardiovascular biomaterials: from function mimicking to endothelialization in vivo

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