Regulation of epithelial to mesenchymal transition in bovine conceptuses through the interaction between follistatin and activin A

Kusama, Kaoru; Bai, Rulan; Ideta, Atsushi; Aoyagi, Yoshito; Okuda, Kiyoshi; Imakawa, Kazuhiko

doi:10.1016/j.mce.2016.06.017

Cited by 22 publications

(58 citation statements)

References 43 publications

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“…This was also the day EMT-related molecule expression was found in the bovine uterus, in which non-invasive bovine TE has upregulated mesenchymal markers CDH2, VIM, MMP2 and EMT-related transcription factors SNAI2, ZEB1 and TWIST1 (Yamakoshi et al 2012, Kusama et al 2016b. In addition, the inhibition of PODXL expression has been demonstrated to prevent the TGF-β-induced EMT in human lung adenocarcinoma A549 cells, strongly suggesting that upregulation of PODXL is necessary for EMT to occur (Meng et al 2011).…”

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 58%

“…Among SMAD2, SMAD3 and SMAD4 expression, phosphorylated SMAD2 increased in day 22 conceptuses. In our TE (CT-1) and EEC co-culture system, the treatment with P22 UF or activin A upregulated EMT marker expressions, which were inhibited by FST in bovine trophoblast cells (Kusama et al 2016b). These results suggest that EMT-related molecule expression in day 22 bovine conceptuses could be induced by activin A secreted from the endometrium to the uterine lumen.…”

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 63%

“…In the co-culture of CT-1 cells and EECs with P22 UF or activin A, SNAI2, ZEB1 and TWIST1 expression was also upregulated (Kusama et al 2016b). Mesenchymal CDH2 had been expressed in the co-culture of CT-1 and EECs, but not in the mono-culture of CT-1 cells alone , indicating that TE (CT-1) cells with EECs were altered to the mesenchymal phenotype from the epithelial phenotype.…”

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 93%

“…We identified that FST increased in P20 UF, concurrent with transiently upregulated FST in day 20 conceptuses. In addition, the expression of EMT markers induced by P22 UF or activin A was inhibited by FST in the co-culture system (Kusama et al 2016b). It was reported that TGF-β1, TGF-β2 or TGF-β3 expression in ovine conceptuses and endometrium increase during the peri-attachment period (Doré et al 1996, Imakawa et al 1998.…”

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 95%

“…Follistatin (FST), an inhibitor of activin A and other TGF-β superfamily members, increased in uterine flushing media (UF) obtained from day 20 of gestation (P20), but FST decreased in P22 UF, whereas elevated activin A was found in P22 UF (Kusama et al 2016b). Among SMAD2, SMAD3 and SMAD4 expression, phosphorylated SMAD2 increased in day 22 conceptuses.…”

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 99%

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Continuous model of conceptus implantation to the maternal endometrium

Imakawa

Bai

Fujiwara

et al. 2017

Journal of Endocrinology

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As placental morphology as well as trophoblast characteristics exhibit wide diversity across mammalian species, underling molecules were also thought to vary greatly. In the majority of cases, however, regardless of the mode of implantation, physiological and biochemical processes in conceptus implantation to the maternal endometrium including the kinds of gene expression and their products are now considered to share many similarities. In fact, recent progress has identified that in addition to the hormones, cytokines, proteases and cell adhesion molecules classically characterized, molecules related to lymphocyte homing and epithelial-mesenchymal transition (EMT) are all required for the progression of conceptus implantation to placentation. In this review, therefore, the newest findings are all incorporated into the molecular and cellular events related to conceptus implantation to the maternal endometrium; primarily from non-invasive bovine placentation and also from invasive human implantation.

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Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 58%

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 63%

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 93%

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 95%

Section: Regulation Of Emt-related Molecule Expression During the Permentioning

confidence: 99%

See 3 more Smart Citations

Continuous model of conceptus implantation to the maternal endometrium

Imakawa

Bai

Fujiwara

et al. 2017

Journal of Endocrinology

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Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation

Manjari¹,

Hyder

Kapoor³

et al. 2018

J of Cellular Biochemistry

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Interferon-τ (IFNT) is a major cytokine produced by the ruminant trophoectoderm during the peri-implantation period exerting immunomodulatory actions on various cells including neutrophils. The current in vitro study was undertaken to analyze the concentration-dependent effects of IFNT on neutrophil gene dynamics to understand its possible role in implantation process. The neutrophils were isolated from the blood of heifers and were cultured subjecting them to different IFNT concentrations (1, 5, or 10 ng/mL). The gene-expression patterns of different interferon-stimulated genes, l-selectin, CD31, CD11b, and progesterone-induced blocking factor (PIBF) were analyzed by quantitative real-time PCR. It was observed that at lower concentrations of IFNT, the IFI16, l-selectin, ISG15, and PIBF were upregulated, whereas at higher concentrations the same were down regulated. At all the experimental concentrations, IFI44, OAS1, MX genes were significantly upregulated and CD31, CD11b were significantly downregulated. At lower concentrations of IFNT, the neutrophil activity with respect to chemoattraction is stimulated, whereas at higher concentrations the same is reduced. Hence, it can be concluded that IFNT exerts concentration-dependent actions on neutrophil gene-expression dynamics indicating a fine modulation of its activity depending upon the temporal variation in its destined functions ultimately leading to successful implantation.

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