2004
DOI: 10.1677/joe.0.1800497
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Regulation of estrogen receptor-alpha expression by the tumor suppressor gene p53 in MCF-7 cells

Abstract: The results presented here demonstrate that p53 upregulates estrogen receptor-(ER ) expression in the human breast cancer cell line MCF-7. Two approaches were used to alter the activity of p53 in the cells. In the first approach, stable transfectants expressing an antisense p53 were established. In the stable clones, expression of antisense p53 resulted in a decrease in the expression of ER protein. In the second approach, MCF-7 cells were transiently transfected with wild-type p53. Overexpression of p53 incre… Show more

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Cited by 68 publications
(63 citation statements)
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“…These results suggest that either EL /LMW-Es induced p53 is not capable of ER down regulation or the cyclin E stimulation of ER expression can override the p53 effect. The discrepancy between our results and previous investigations could be explained by using different silencing technology (full length antisense RNA of p53) by other researchers (Angeloni et al, 2004). Since the presence of apoptotic protein can determine the susceptibility of cancer cells to chemotherapy, we have studied the expression of the Bcl-2 and Bax in our stable clones.p53 is a regulator of Bax and Bcl-2 gene expression.…”
Section: Discussioncontrasting
confidence: 78%
See 1 more Smart Citation
“…These results suggest that either EL /LMW-Es induced p53 is not capable of ER down regulation or the cyclin E stimulation of ER expression can override the p53 effect. The discrepancy between our results and previous investigations could be explained by using different silencing technology (full length antisense RNA of p53) by other researchers (Angeloni et al, 2004). Since the presence of apoptotic protein can determine the susceptibility of cancer cells to chemotherapy, we have studied the expression of the Bcl-2 and Bax in our stable clones.p53 is a regulator of Bax and Bcl-2 gene expression.…”
Section: Discussioncontrasting
confidence: 78%
“…Previous reports including pathoclincal and molecular ones indicate that transfection with antisense p53expression vector disrupts ER expression (Angeloni et al, 2004;Panahi et al, 2013). However; our results indicate that both EL/LMW-Es overexpression and p53 ablation induces ER expression.…”
Section: Discussioncontrasting
confidence: 59%
“…These studies suggest that p53 not only regulates the ER-α promoter but may play a role in determining ER status. It may be postulated that p53 plays a role in the progression of breast cancer from a hormone-dependent phenotype to a more aggressive phenotype that is unresponsive to hormones [60]. Furthermore, it has been found that the treatment of MCF-7 cells with paclitaxel (Taxol ® ; Bristol-Myers Squibb, Princeton, NJ, http://www.bms.com) results in the induction of ER-α gene transcription, which may be mediated through the induction of p53 [61].…”
Section: Epigenetic Information and Er-α Expression In Breast Cancermentioning
confidence: 99%
“…Several clinical observations indicate a role for oestrogen and ER in the development, progression and treatment of human breast cancer (Angeloni et al, 2004). In addition, there is substantial evidence showing that alterations in the tumour suppressor gene, p53, are associated with the development of several types of cancer, including breast cancer (Hollstein et al, 1991).…”
Section: Discussionmentioning
confidence: 99%