Regulation of ethylene-related gene expression by indole-3-acetic acid and 4-chloroindole-3-acetic acid in relation to pea fruit and seed development

Jayasinghege, Charitha P. A.; Ozga, Jocelyn A.; Waduthanthri, Kosala D.; Reinecke, Dennis M.

doi:10.1093/jxb/erx217

Cited by 11 publications

(17 citation statements)

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“…In pea, pericarp growth arrests by 1 DAA in the absence of pollination but pericarps remain green and turgid through to 3 DAA (Jayasinghege et al , 2017). In non-pollinated pericarps, PsTIR1b transcript abundance was elevated ~2-fold by 1 DAA and remained elevated through to 3 DAA in all pericarp tissues compared to pollinated fruits (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…I 3 (Alaska-type) was used for all pea studies. Plants were grown in a growth chamber with a 16/8 h light/dark photoperiod at 19/17 °C as described by Jayasinghege et al (2017).…”

Section: Methodsmentioning

confidence: 99%

“…A TaqMan One-Step RT-PCR Master-Mix Reagents Kit or RNA-to-Ct 1-Step Kit (Applied Biosystems) was used for qRT-PCR analysis. The reactions were performed in a StepOnePlus Real-Time PCR system as described by Jayasinghege et al (2017; Supplementary Protocol S2).…”

Section: Methodsmentioning

confidence: 99%

“…In other species, such as tomato ( Solanum lycopersicum ; Serrani et al , 2008) and Arabidopsis (Dorcey et al , 2009), IAA stimulates fruit growth via GAs. Furthermore, in pea 4-Cl-IAA, but not IAA, also inhibits the ethylene response in deseeded pericarps, potentially via the inhibition of ethylene signaling (Johnstone et al , 2005; Jayasinghege et al , 2017), enhancing the promotion of pericarp growth. These differential interactions of IAA and 4-Cl-IAA with the GA and ethylene biosynthesis and signaling pathways likely play a primary role in determining the effects of the two auxins on pea pericarp development.…”

Section: Introductionmentioning

confidence: 99%

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TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Jayasinghege

Ozga

Nadeau

et al. 2019

Journal of Experimental Botany

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Section: Resultsmentioning

confidence: 99%

“…I 3 (Alaska-type) was used for all pea studies. Plants were grown in a growth chamber with a 16/8 h light/dark photoperiod at 19/17 °C as described by Jayasinghege et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Jayasinghege

Ozga

Nadeau

et al. 2019

Journal of Experimental Botany

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“…Both 4-Cl-IAA and 4-CF -IAA promote root formation in black gram (Vigna mungo) [13]. It is recently reported that 4-Cl-IAA stimulates pericarp growth and gibberellin biosynthesis in Pisum sativum [23]. Although how 4-Cl-IAA affects grapevines needs further investigation, it might be possible that 4-Cl-IAA also promotes vines' root formation as in black gram.…”

Section: Discussionmentioning

confidence: 99%

Production of Virus-free Vitis Vinifera ‘koshu’ Plantlets From Shoot Tips or Axillary Buds 

Dosho¹,

Ando²,

Tsutsumiuchi³

et al. 2020

Preprint

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Objective: Vitis vinifera ‘Koshu’ is the most important grape cultivar for producing white wine in Japan. Most Koshu vines are heavily infected with viruses of multiple types, such as grapevine leafroll-associated viruses, which severely interfere with normal development and physiology such as photosynthesis of grapes. The meristem culture method has been the most commonly and successfully employed to eliminate viruses from grapevines. Koshu is, however, known for its difficulty of regenerating plantlets from meristems. Results: We have established a robust method of regenerating plantlets with high frequency from shoot apical meristem tissues of ‘Koshu’ grapevines. The key points of this method are the use of (1) no or low concentrations of 6-benzyl-aminopurine (BAP) (0.22 µM) for shoot regeneration, and (2) a synthetic auxin, such as 10-5 M 4-chloroindole-3-acetic acid (4-Cl-IAA), for rooting. This method should be very useful and important for producing ‘Koshu’ virus-free vines.

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Heat stress differentially modifies ethylene biosynthesis and signaling in pea floral and fruit tissues

et al. 2017

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Ethylene biosynthesis is regulated in reproductive tissues in response to heat stress in a manner to optimize resource allocation to pollinated fruits with developing seeds. High temperatures during reproductive development are particularly detrimental to crop fruit/seed production. Ethylene plays vital roles in plant development and abiotic stress responses; however, little is known about ethylene's role in reproductive tissues during development under heat stress. We assessed ethylene biosynthesis and signaling regulation within the reproductive and associated tissues of pea during the developmental phase that sets the stage for fruit-set and seed development under normal and heat-stress conditions. The transcript abundance profiles of PsACS [encode enzymes that convert S-adenosyl-L-methionine to 1-aminocyclopropane-1-carboxylic acid (ACC)] and PsACO (encode enzymes that convert ACC to ethylene), and ethylene evolution were developmentally, environmentally, and tissue-specifically regulated in the floral/fruit/pedicel tissues of pea. Higher transcript abundance of PsACS and PsACO in the ovaries, and PsACO in the pedicels was correlated with higher ethylene evolution and ovary senescence and pedicel abscission in fruits that were not pollinated under control temperature conditions. Under heat-stress conditions, up-regulation of ethylene biosynthesis gene expression in pre-pollinated ovaries was also associated with higher ethylene evolution and lower retention of these fruits. Following successful pollination and ovule fertilization, heat-stress modified PsACS and PsACO transcript profiles in a manner that suppressed ovary ethylene evolution. The normal ethylene burst in the stigma/style and petals following pollination was also suppressed by heat-stress. Transcript abundance profiles of ethylene receptor and signaling-related genes acted as qualitative markers of tissue ethylene signaling events. These data support the hypothesis that ethylene biosynthesis is regulated in reproductive tissues in response to heat stress to modulate resource allocation dynamics.

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Regulation of ethylene-related gene expression by indole-3-acetic acid and 4-chloroindole-3-acetic acid in relation to pea fruit and seed development

Abstract: Modulation of ethylene biosynthesis and signaling pathways is a key node for seed and auxin regulation of pea fruit growth and development.

Cited by 11 publications

References 50 publications

TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Production of Virus-free Vitis Vinifera ‘koshu’ Plantlets From Shoot Tips or Axillary Buds

Heat stress differentially modifies ethylene biosynthesis and signaling in pea floral and fruit tissues

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Regulation of ethylene-related gene expression by indole-3-acetic acid and 4-chloroindole-3-acetic acid in relation to pea fruit and seed development

Abstract: Modulation of ethylene biosynthesis and signaling pathways is a key node for seed and auxin regulation of pea fruit growth and development.

Cited by 11 publications

References 50 publications

TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Production of Virus-free Vitis Vinifera ‘koshu’ Plantlets From Shoot Tips or Axillary Buds&nbsp;

Heat stress differentially modifies ethylene biosynthesis and signaling in pea floral and fruit tissues

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Production of Virus-free Vitis Vinifera ‘koshu’ Plantlets From Shoot Tips or Axillary Buds