Regulation of expression of mouse interferon‐induced transmembrane protein like gene‐3, <i>Ifitm3</i> (<i>mil‐1, fragilis</i>), in germ cells

Tanaka, Satomi; Nagamatsu, Go; Tokitake, Yuko; Kasa, Miyuki; Tam, Patrick; Matsui, Yasuhisa

doi:10.1002/dvdy.20085

Cited by 71 publications

(69 citation statements)

References 23 publications

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“…When 35 S-labeled IFITM3-containing lysates were incubated with unprogrammed non-radioactive lysates and then immunoprecipitated with anti-OPN, no radioactive IFITM3 protein of 14 kDa was observed on the resultant polyacrylamide gels (Figure 1c, lanes 4). This radioactive band was not observed in controls in which an unrelated transcription factor Tip 60-HA (Brady et al, 1999) (Figure 1c (Tanaka et al, 2004(Tanaka et al, , 2005. We transfected R37 cells with pBK-CMVTip 60 (Tip 60) (Brady et al, 1999) with or without IFITM3 to generate R37-Tip 60 and R37-Tip60-IFITM3 cells, respectively.…”

Section: Resultsmentioning

confidence: 91%

Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression

et al. 2009

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Osteopontin is a secreted, integrin-binding and phosphorylated acidic glycoprotein, which has an important role in tumour progression. We have shown that Wnt, Ets, AP-1, c-jun and b-catenin/Lef-1/Tcf-1 stimulates OPN transcription in rat mammary carcinoma cells by binding to a specific promoter sequence. However, co-repressors of OPN have not been identified. In this study, we have used the bacterial two-hybrid system to isolate cDNA-encoding proteins that bind to OPN and modulate its role in malignant transformation. Using this approach we isolated interferon-induced transmembrane protein 3 gene (IFITM3) as a potential protein partner. We show that IFITM3 and OPN interact in vitro and in vivo and that IFITM3 reduces osteopontin (OPN) mRNA expression, possibly by affecting OPN mRNA stability. Stable transfection of IFITM3 inhibits OPN, which mediates anchorage-independent growth, cell adhesion and cell invasion. Northern blot analysis revealed an inverse mRNA expression pattern of IFITM3 and OPN in human mammary cell lines. Inhibition of IFITM3 by antisense RNA promoted OPN protein expression, enhanced cell invasion by parental benign non-invasive Rama 37 cells, indicating that the two proteins interact functionally as well. We also identified an IFITM3 DNA-binding domain, which interacts with OPN, deletion of which abolished its inhibitive effect on OPN. This work has shown for the first time that IFITM3 physically interacts with OPN and reduces OPN mRNA expression, which mediates cell adhesion, cell invasion, colony formation in soft agar and metastasis in a rat model system.

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Section: Resultsmentioning

confidence: 91%

Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression

et al. 2009

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“…Fluorescence activated cell sorting (FACS) was used to isolate GFP-expressing PGCs from IFITM3 (mil1)-GFP (3.0G) transgenic E12.5 embryos (Tanaka et al, 2004); these PGCs were plated into 24-well culture dishes with Sl/Sl4-m220 feeder cells expressing membrane-bound Steel factor (Matsui et al, 1991). At the end of the culture period, colonies with alkaline phosphatase (ALP)-positive EGCs were counted, and the efficiency of EGC formation was determined as a ratio of the number of EGC colonies to every 100 sorted PGCs that were seeded in a culture well.…”

Section: Effects Of Culture Conditions On the Efficiency Of Egc Formamentioning

confidence: 99%

“…The mil-1-GFP (3.0G) (Tanaka et al, 2004) and Oct4-deltaPE-GFP (Yoshimizu et al, 1999) transgenic mice were maintained in a C57BL/6J genetic background. AKT-MER transgenic mice (Kimura et al, 2008) were maintained in a B6D2F1 genetic background.…”

Section: Collection Of Embryosmentioning

confidence: 99%

The majority of early primordial germ cells acquire pluripotency by AKT activation

et al. 2014

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Primordial germ cells (PGCs) are undifferentiated germ cells in embryos, the fate of which is to become gametes; however, mouse PGCs can easily be reprogrammed into pluripotent embryonic germ cells (EGCs) in culture in the presence of particular extracellular factors, such as combinations of Steel factor (KITL), LIF and bFGF (FGF2). Early PGCs form EGCs more readily than do later PGCs, and PGCs lose the ability to form EGCs by embryonic day (E) 15.5. Here, we examined the effects of activation of the serine/threonine kinase AKT in PGCs during EGC formation; notably, AKT activation, in combination with LIF and bFGF, enhanced EGC formation and caused ∼60% of E10.5 PGCs to become EGCs. The results indicate that the majority of PGCs at E10.5 could acquire pluripotency with an activated AKT signaling pathway. Importantly, AKT activation did not fully substitute for bFGF and LIF, and AKT activation without both LIF and bFGF did not result in EGC formation. These findings indicate that AKT signal enhances and/or collaborates with signaling pathways of bFGF and of LIF in PGCs for the acquisition of pluripotency.

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“…At a similar time screening of gonadal germ cells also resulted in identification of these genes (Sato et al, 2002;Tanaka and Matsui, 2002). Fragilis (also known as Mil1 or Inftn3) encodes an interferon inducible transmembrane protein important for cell-cell adhesion of germ cell progenitors (Saitou et al, 2002;Lange et al, 2003;Tanaka et al, 2004;Tanaka et al, 2005). Stella (also known as Pgc7) encodes a protein containing a putative SAP domain, which may function in the regulation of chromatin (Saitou et al, 2002;Sato et al, 2002).…”

Section: Origin Of Mouse Germ Cells: Establishing and Maintaining Undmentioning

confidence: 99%

Foetal germ cells: striking the balance between pluripotency and differentiation

Western¹

2009

Int. J. Dev. Biol.

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Regulation of expression of mouse interferon‐induced transmembrane protein like gene‐3, Ifitm3 (mil‐1, fragilis), in germ cells

Cited by 71 publications

References 23 publications

Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression

Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression

The majority of early primordial germ cells acquire pluripotency by AKT activation

Foetal germ cells: striking the balance between pluripotency and differentiation

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