Regulation of expression of HGF in BM‐MSCs by baculovirus‐mediated transduction

Abstract

Bone marrow-derived mesenchymal stem cells (BM-MSCs) transplantation is widely adopted for the curing of osteonecrosis of femoral head (ONFH) in recent years. Furthermore, it is known that introducing hepatocyte growth factor (HGF) into BM-MSCs will greatly improve the therapeutic effect of stem-cell therapy owing to the great angiogenic and anti-fibrotic capabilities of HGF. However, continuing overexpression of HGF in vivo may cause sarcomas, such as Kaposi's sarcoma. Aiming at enhancing the therapeutic effect and preventing the side effects of HGF-modified stem-cell transplantation on ONFH, we sought to construct a gene regulation system to control HGF expression in BM-MSCs rigorously and accurately. We selected baculovirus as the gene vector and introduced pTet-on advanced system into that. Finally, a virus vector vAc(rtTA2s-Ptight-HGF) was successfully built and delivered into BM-MSCs to regulate the accurate expression of HGF. As shown in the results, different levels of HGF expression were verified by ELISA and Western blot with different induction doses of doxycycline (DOX). There was a dose-response relationship between them, and the optimum dose of DOX to induce HGF expression in BM-MSCs in vitro was 1 µg/mL. We conclude that it is feasible to regulate HGF expression in BM-MSCs by baculovirus-mediated one-off transduction.

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

“…Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration (Nakamura et al, 2011;Tu et al, 2013). In our study, transcripts of HGF where found significantly up-regulated in cells treated with NannoG in comparison to the control cells.…”

Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro

“…These findings suggest that UC-MSCs may secrete soluble factors that inhibit V γ 9V δ 2T cell proliferation. MSCs can secrete many factors, including transforming growth factor- β (TGF- β ) [ 30 ], hepatic growth factors (HGF) [ 31 ], prostaglandin E2 (PGE2) [ 30 ], IL-10 [ 30 ], indolamine 2,3-dioxygenase (IDO) [ 32 , 33 ], nitric oxide (NO) [ 30 ], heme oxygenase-1 (HO-1) [ 34 ], and human leukocyte antigen-G (HLA-G) [ 35 ], and TGF- β , IL-10, and PGE2 are potent inhibitors of T cell immunity [ 4 , 36 – 38 ]. These factors together with other unknown factors may act to inhibit T cell proliferation directly and indirectly.…”