Regulation of FA and TAG biosynthesis pathway genes in endosperms and embryos of high and low oil content genotypes of Jatropha curcas L

Sood, Archit; Chauhan, Rajinder Singh

doi:10.1016/j.plaphy.2015.06.015

Cited by 25 publications

(24 citation statements)

References 65 publications

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“…The accumulation of seed oil is mainly provided by TAG biosynthesis (Li et al, 2011; Troncosoponce et al, 2011; Hu et al, 2013; Sood and Chauhan, 2015). The pathway and related genes of lipid biosynthesis have been studied in many plants (Beisson et al, 2003; Bates et al, 2009; Yin et al, 2013; Jang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Among them, acetyl-CoA carboxylase (ACCase) (E.C. 6.4.1.2) is an important enzyme, which catalyze acetyl-CoA to produce malonyl-CoA, and can be served as a committed step for de novo fatty acid biosynthesis in plastid (Sasaki and Nagano, 2004; Fukuda et al, 2013; Ran et al, 2015; Sood and Chauhan, 2015). In dicotyledon and non-graminaceous monocotyledon plants, plastidial ACCase is a multi-subunit complex comprised of four different polypeptides (biotin carboxyl carrier protein, BCCP; biotin carboxylase, BC; α- and β-carboxyltransferase subunits, α-CT and β-CT) with the exception of rapeseed plastid, which contains multifunctional ACCase comprised of a large multifunctional polypeptide (Elborough et al, 1996; Schulte et al, 1997; Sasaki and Nagano, 2004; Cui et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium

et al. 2017

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Acetyl-CoA carboxylase is an important enzyme, which catalyzes acetyl-CoA’s carboxylation to produce malonyl-CoA and to serve as a committed step for de novo fatty acid biosynthesis in plastids. In this study, 24 putative cotton BCCP genes were identified based on the lately published genome data in Gossypium . Among them, 4, 4, 8, and 8 BCCP homologs were identified in Gossypium raimondii, G. arboreum, G. hirsutum , and G. barbadense , respectively. These genes were divided into two classes based on a phylogenetic analysis. In each class, these homologs were relatively conserved in gene structure and motifs. The chromosomal distribution pattern revealed that all the BCCP genes were distributed equally on corresponding chromosomes or scaffold in the four cotton species. Segmental duplication was a predominant duplication event in both of G. hirsutum and G. barbadense. The analysis of the expression profile showed that 8 GhBCCP genes expressed in all the tested tissues with changed expression levels, and GhBCCP genes belonging to class II were predominantly expressed in developing ovules. Meanwhile, the expression analysis for the 16 cotton BCCP genes from G. raimondii, G. arboreum and G. hirsutum showed that they were induced or suppressed by cold or salt stress, and their expression patterns varied among different tissues. These findings will help to determine the functional and evolutionary characteristics of the BCCP genes in Gossypium species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium

et al. 2017

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“…The benzoin embryo and endosperm accumulated an equal amount of oil bodies at 120 DAF (Figure 3a,b), but the dry weight and tissue volume of the endosperm was higher (data not shown). On the contrary, the lipid content of the embryo is relatively low as compared to that of the endosperm and seed coat in the Jatropha curcas mature seed [19,26,27]. Although ROA would not necessarily represent the cell lipid content level, our collective evidence indicated the different oil deposition patterns within the two main tissues.…”

Section: Endosperm and Embryo Cells Exhibit Differential Oil Depositimentioning

confidence: 64%

“…In albuminous oilseeds, oil bodies can be found in the endosperm and embryo [10]. For high oil content genotypes of Jatropha curcas seeds, a higher expression of most of the fatty acid and TAG synthetic pathway genes was shown in endosperms compared to embryos, whereas, in low oil content genotypes, a significant difference was not found between endosperms and embryos [19]. This indicates the fundamental function of oilseed endosperms in determining the whole kernel lipid content.…”

Section: Introductionmentioning

confidence: 93%

“…The kernel ROA dynamic turned out to be closely correlated to endosperm cells, which again suggested a reduced oil accumulation rate during benzoin seed development. Notably, cotyledon cells, which are the main storage tissues and the main location of TAG accumulation on the whole-embryo level [19], were selected to calculate the embryo ROA in the present study; the actual embryo ROA value, therefore, could be lower than the determined value.…”

Section: Endosperm and Embryo Cells Exhibit Differential Oil Depositimentioning

confidence: 99%

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Quantitative Spatiotemporal Oil Body Ultrastructure Helps to Verify the Distinct Lipid Deposition Patterns in Benzoin Endosperm and Embryo Cells

Zhang

Luo

Wang

et al. 2018

Forests

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Seed oil content is an important characteristic for the potential biofuel feedstock benzoin (Styrax tonkinensis). With the aim of further understanding benzoin lipid biosynthesis, the endosperm and embryo cell ultrastructures were acquired through transmission electron microscopy (TEM); the relative oil body area per cell (ROA) and oil body diameter (OBD) were then calculated by analyzing TEM images via computer software. The endosperm ROA peaked at 99 days after flowering (DAF) (79.04%), and the embryo ROA dynamic fitted the "S" curve. Significant linear relations (p < 0.01) were only observed between endosperm ROAs and the contents of whole-kernel crude lipid and fatty acids. The endosperm OBD (1.18-2.43 µm) was larger than that of embryo OBD (0.38-0.77 µm). M-shaped dynamics of acetyl coenzyme carboxylase (ACC) and diglyceride acyltransferase (DGAT) activities resembled the dynamic of endosperm OBD, as two peaks were observed at 78 and 113 DAF; the big oil body (≥1.8 µm) fraction in endosperm increased as kernel ACC and DGAT activities also increased, and vice-versa. Embryo OBD gradually increased, with the big oil body (≥0.5 µm) proportion increasing and the small oil body (<0.5 µm) proportion decreasing in general. Our results collectively suggested the distinct lipid accumulation patterns in the two benzoin kernel tissues, and revealed that the endosperm may determine the whole kernel oil biosynthetic process.

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Transgenic and Genome Editing Approaches for Modifying Plant Oils

Wayne¹,

Gachotte²,

Walsh³

2018

Methods in Molecular Biology

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Regulation of FA and TAG biosynthesis pathway genes in endosperms and embryos of high and low oil content genotypes of Jatropha curcas L

Cited by 25 publications

References 65 publications

Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium

Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium

Quantitative Spatiotemporal Oil Body Ultrastructure Helps to Verify the Distinct Lipid Deposition Patterns in Benzoin Endosperm and Embryo Cells

Transgenic and Genome Editing Approaches for Modifying Plant Oils

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