Regulation of flagellar motility and biosynthesis in enterohemorrhagic
            <i>Escherichia coli</i>
            O157:H7

Sun, Hongmin; Wang, Min; Liu, Yutao; Wu, Pan; Yao, Ting; Yang, Wen; Yang, Qian; Yan, Jun; Yang, Bin

doi:10.1080/19490976.2022.2110822

Cited by 28 publications

(15 citation statements)

References 116 publications

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“…Flagellar biosynthesis is a process that requires 2% of the cell’s biosynthetic resources [ 3 ]. Genes for flagellum synthesis are usually organized into a cluster that encodes transcription factors, basal body and hook proteins of the flagellum, the filament of the flagellum (FliC), as well as proteins necessary for motility and chemotaxis [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Vilas Boas,

Castro,

Araújo

et al. 2024

Microorganisms

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Flagellum-mediated motility has been suggested to contribute to virulence by allowing bacteria to colonize and spread to new surfaces. In Salmonella enterica and Escherichia coli species, mutants affected by their flagellar motility have shown a reduced ability to form biofilms. While it is known that some species might act as co-aggregation factors for bacterial adhesion, studies of food-related biofilms have been limited to single-species biofilms and short biofilm formation periods. To assess the contribution of flagella and flagellum-based motility to adhesion and biofilm formation, two Salmonella and E. coli mutants with different flagellar phenotypes were produced: the fliC mutants, which do not produce flagella, and the motAB mutants, which are non-motile. The ability of wild-type and mutant strains to form biofilms was compared, and their relative fitness was determined in two-species biofilms with other foodborne pathogens. Our results showed a defective and significant behavior of E. coli in initial surface colonization (p < 0.05), which delayed single-species biofilm formation. Salmonella mutants were not affected by the ability to form biofilm (p > 0.05). Regarding the effect of motility/flagellum absence on bacterial fitness, none of the mutant strains seems to have their relative fitness affected in the presence of a competing species. Although the absence of motility may eventually delay initial colonization, this study suggests that motility is not essential for biofilm formation and does not have a strong impact on bacteria’s fitness when a competing species is present.

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Section: Introductionmentioning

confidence: 99%

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Vilas Boas,

Castro,

Araújo

et al. 2024

Microorganisms

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“…Both fresh-cut produce from indoor plant factories and meal kits contain various vegetables that usually consume without processing. Escherichia coli O157:H7 is known as the most common pathogen in fresh produce and cause diseases in human such as hemorrhagic colitis, bloody diarrhea, and hemolytic uremic syndrome 12 . From 2008 to 2020 a total of 515, 165, and 235 cases of foodborne outbreaks from fresh produce were reported due to enterohemorrhagic E. coli (EHEC) in the US, the United Kingdom (UK), and Canada, respectively 13 – 15 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptional response of Escherichia coli O157:H7 to light-emitting diodes with various wavelengths

Khan

Kim

Yuk

2023

Sci Rep

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We investigated the physiological and transcriptomic response of Escherichia coli at the early stationary phase to light-emitting diodes with different wavelengths. The growth and metabolic changes of E. coli O157:H7 were examined under the influence of 465, 520, and 625 nm illuminated light. Under 465 nm illumination, the growth of E. coli O157:H7 was significantly retarded compared to 520 nm and 625 nm illumination and non-illuminated control. Metabolic changes were examined under these illumination and non-illuminated conditions based on transcriptomic reads. Transcriptomic response under 520 nm and 625 nm remained almost similar to control except few up-and down-regulated genes. Carbohydrates metabolic transcriptomic reads were greatly down-regulated under 465 nm illumination compared to 520 nm and 625 nm illumination and non-illuminated control showing depletion of glucose as a sole energy source during the exponential phase. Fatty acid degradation such as fad regulon-related genes was up-regulated in cells under 465 nm illumination revealing the shifting of cells to use fatty acid as a new carbon energy source during the early stationary phase. Exposure of E. coli O157:H7 cells to 465 nm illuminated light down-regulated virulence factor genes such as hlyA, hlyB, hlyC, stx1A, stx2B, paa, and bdm. Under the stress of 465 nm illumination, expression of stress and flagellar motility-related genes were up-regulated causing consumption of energy and reduction in cell growth. Also, oxidative phosphorylated transcriptomic reads were up-regulated under 465 nm illumination probably due to the production of ROS that might involve in the reduction of cell growth during the early stationary phase. These results indicate that pathogenic E. coli O157:H7 respond differentially to a different wavelength of the light-emitting diodes used in this study.

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“…Both fresh-cut produce from indoor plant factories and meal kits contain various vegetables that usually consume without processing. Escherichia coli O157:H7 is known as the most common pathogen in fresh produce and cause diseases in human such as hemorrhagic colitis, bloody diarrhea, and hemolytic uremic syndrome 15 . From 2008-2020 a total of 515, 165, and 235 cases of foodborne outbreaks from fresh produce were reported due to enterohemorrhagic E. coli (EHEC) in the US, the United Kingdom (UK), and Canada, respectively [16][17][18][19][20] .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptional response of Escherichia coli O157:H7 to light- emitting diodes with various wavelengths

Khan

Kim

Yuk

2022

Preprint

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We investigated the physiological and transcriptomic response of Escherichia coli at the early stationary phase to light-emitting diodes with different wavelengths. The objective of this study was to investigate the effects of 465, 520, and 625 nm illuminated light on the growth and metabolic changes of E. coli O157:H7. Under 465 nm illumination, the growth of E. coli O157:H7 was significantly retarded compared to 520 nm and 625 nm illumination and non-illuminated control. Metabolic changes were examined under these illumination and non-illuminated conditions based on transcriptomic reads. Transcriptomic response under 520 nm and 625 nm remained almost similar to control except few up-and down-regulated genes. Carbohydrates metabolic transcriptomic reads were greatly down-regulated under 465 nm illumination compared to 520 nm and 625 nm illumination and non-illuminated control showing depletion of glucose as a sole energy source during the exponential phase. Fatty acid degradation such as fad regulon-related genes was up-regulated in cells under 465 nm illumination revealing the shifting of cells to use fatty acid as a new carbon energy source during the early stationary phase. Exposure of E. coli O157:H7 cells to 465 nm illuminated light down-regulated virulence factor genes such as hlyA, hlyB, hlyC, stx1A, stx2B, paa, and bdm. Under the stress of 465 nm illumination, expression of stress and flagellar motility-related genes were up-regulated causing consumption of energy and reduction in cell growth. Also, oxidative phosphorylated transcriptomic reads were up-regulated under 465 nm illumination probably due to the production of ROS that might involve in the reduction of cell growth during the early stationary phase. These results indicate that pathogenic E. coli O157:H7 respond differentially to a different wavelength of the light-emitting diodes used in this study.

show abstract

Regulation of flagellar motility and biosynthesis in enterohemorrhagic Escherichia coli O157:H7

Cited by 28 publications

References 116 publications

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Genome-wide transcriptional response of Escherichia coli O157:H7 to light-emitting diodes with various wavelengths

Genome-wide transcriptional response of Escherichia coli O157:H7 to light- emitting diodes with various wavelengths

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