Regulation of floral scent production in petunia revealed by targeted metabolomics

Verdonk, Julian C.; Vos, C. H. Ric De; Verhoeven, H. A.; Haring, Michel A.; Tunen, Arjen J. van; Schuurink, Robert C.

doi:10.1016/s0031-9422(02)00707-0

Cited by 250 publications

(233 citation statements)

References 33 publications

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“…and accessions. Rhythmic emissions of methyl benzoate, benzaldehyde, isoeugenol, benzeneacetaldehyde and benzyl benzoate have also been detected in Petunia hybrida (Kolosova et al 2001;Verdonk et al 2003;Negre et al 2003). The non-rhythmic emission of hexadecadienal methyl ester and Z-9,17-octadecadienol (Fig.…”

Section: P Axillaris Flower Emission Is Nocturnal and Controlled Bymentioning

confidence: 90%

“…and accessions. Flower-odours of cultivated Petunia hybrida (cv Mitchell) have been collected in previous studies (Kolosova et al 2001;Verdonk et al 2003;Boatright et al 2004), but no information is available for the flower-odours of wild Petunia species, apart from our previous work on the differences between the odour blends of P. integrifolia inflata vs. P. axillaris parodii wild species (Stuurman et al 2004). We collected the odours of different accessions for both wild species to find out if they release similar flower-odour blends.…”

Section: Introductionmentioning

confidence: 99%

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The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta

Hoballah

Stuurman

Turlings

et al. 2005

Planta

165

154

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The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta Abstract In the genus Petunia, distinct pollination syndromes may have evolved in association with bee-visitation (P. integrifolia spp.) or hawk moth-visitation (P. axillaris spp). We investigated the extent of congruence between floral fragrance and olfactory perception of the hawk moth Manduca sexta. Hawk moth pollinated P. axillaris releases high levels of several compounds compared to the bee-pollinated P. integrifolia that releases benzaldehyde almost exclusively. The three dominating compounds in P. axillaris were benzaldehyde, benzyl alcohol and methyl benzoate. In P. axillaris, benzenoids showed a circadian rhythm with an emission peak at night, which was absent from P. integrifolia. These characters were highly conserved among different P. axillaris subspecies and P. axillaris accessions, with some differences in fragrance composition. Electroantennogram (EAG) recordings using flower-blends of different wild Petunia species on female M. sexta antennae showed that P. axillaris odours elicited stronger responses than P. integrifolia odours. EAG responses were highest to the three dominating compounds in the P. axillaris flower odours. Further, EAG responses to odour-samples collected from P. axillaris flowers confirmed that odours collected at night evoked stronger responses from M. sexta than odours collected during the day. These results show that timing of odour emissions by P. axillaris is in tune with nocturnal hawk moth activity and that flower-volatile composition is adapted to the antennal perception of these pollinators.

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Section: P Axillaris Flower Emission Is Nocturnal and Controlled Bymentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta

Hoballah

Stuurman

Turlings

et al. 2005

Planta

165

154

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“…Previously we demonstrated that PhADT1 downregulation in Petunia hybrida flowers, which emit high levels of phenylalaninederived volatiles 24,25 , resulted in a greater decrease in phenylalanine levels compared with the effect of PhPPA-AT downregulation (up to 82% versus 20% reduction, respectively) 16,17 . Such a differential impact on phenylalanine levels from the downregulation of these individual genes was suggested to be due to either sufficient residual PPA-AT activity in the PhPPA-AT RNAi lines capable of sustaining the arogenate pathway, or the redirection of carbon flux from accumulated prephenate in the PhPPA-AT RNAi lines through the hitherto undetected alternative phenylpyruvate pathway 17 .…”

mentioning

confidence: 88%

An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase

et al. 2013

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Phenylalanine is a vital component of proteins in all living organisms, and in plants is a precursor for thousands of additional metabolites. Animals are incapable of synthesizing phenylalanine and must primarily obtain it directly or indirectly from plants. Although plants can synthesize phenylalanine in plastids through arogenate, the contribution of an alternative pathway via phenylpyruvate, as occurs in most microbes, has not been demonstrated. Here we show that plants also utilize a microbial-like phenylpyruvate pathway to produce phenylalanine, and flux through this route is increased when the entry point to the arogenate pathway is limiting. Unexpectedly, we find the plant phenylpyruvate pathway utilizes a cytosolic aminotransferase that links the coordinated catabolism of tyrosine to serve as the amino donor, thus interconnecting the extra-plastidial metabolism of these amino acids. This discovery uncovers another level of complexity in the plant aromatic amino acid regulatory network, unveiling new targets for metabolic engineering.

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“…Tissues were harvested as described above. Total RNA was isolated with a Phenol-LiCl based method as described by Verdonk et al (2003). RT-PCR determination of transcript levels was done as described by Ament et al (2004).…”

Section: Rt-pcr Analysismentioning

confidence: 99%

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

Ament

Schie

Bouwmeester³

et al. 2006

Planta

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Two cDNAs encoding geranylgeranyl pyrophosphate (GGPP) synthases from tomato (Lycopersicon esculentum) have been cloned and functionally expressed in Escherichia coli. LeGGPS1 was predominantly expressed in leaf tissue and LeGGPS2 in ripening fruit and Xower tissue. LeGGPS1 expression was induced in leaves by spider mite (Tetranychus urticae)-feeding and mechanical wounding in wild type tomato but not in the jasmonic acid (JA)-response mutant def-1 and the salicylic acid (SA)-deWcient transgenic NahG line. Furthermore, LeGGPS1 expression could be induced in leaves of wild type tomato plants by JA-or methyl salicylate (MeSA)-treatment. In contrast, expression of LeGGPS2 was not induced in leaves by spider mite-feeding, wounding, JA-or MeSA-treatment. We show that emission of the GGPP-derived volatile terpenoid (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) correlates with expression of LeGGPS1. An exception was MeSA-treatment, which resulted in induction of LeGGPS1 but not in emission of TMTT. We show that there is an additional layer of regulation, because geranyllinalool synthase, catalyzing the Wrst dedicated step in TMTT biosynthesis, was induced by JA but not by MeSA.

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Regulation of floral scent production in petunia revealed by targeted metabolomics

Cited by 250 publications

References 33 publications

The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta

The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta

An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

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