Regulation of floral senescence in Arabidopsis by coordinated action of CONSTANS and jasmonate signaling

Serrano-Bueno, Gloria; Reyes, Pedro de los; Chini, Andrea; Ferreras-Garrucho, Gabriel; Medina-Hernández, Víctor Sánchez de; Botër, Marta; Solano, Roberto; Valverde, Federico

doi:10.1016/j.molp.2022.09.017

Cited by 20 publications

(14 citation statements)

References 79 publications

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“…Out of the 12, WRKY57 and WRKY60 were selected as our primary candidates to regulate IDA because i) their own upregulation preceded the induction of IDA , and ii) WRKY57 and WRKY60 had been highlighted in the list of most significantly regulated genes of the AZ transcriptome (Cai and Lashbrook, 2008). Besides, WRKY57 is involved in balancing jasmonic acid and auxin signaling in leaves during senescence, is a negative regulator of biotic stress resistance and its overexpression confers drought tolerance – processes previously linked to IDA signaling and abscission (Jiang et al, 2012; Jiang et al, 2014; Jiang and Yu, 2016; Serrano-Bueno et al, 2022). Similarly, WRKY60 is involved in immunity, and ABA signaling in osmotic and salt stress (Xu et al, 2006; Chen et al, 2010; Liu et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

Galindo-Trigo,

Bågman,

Ishida

et al. 2023

Preprint

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Plants shed organs like leaves, petals or fruits through the process of abscission. Monitoring cues like age, resource availability, biotic and abiotic stresses allows plants to abscise organs in a timely manner. How these signals are integrated in the molecular pathways that drive abscission is largely unknown. The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene is one of the key regulators of floral organ abscission in Arabidopsis and is known to transcriptionally respond to most abscission-regulating cues. Interrogating the IDA promoter in silico and in vitro we identified transcription factors that can potentially modulate IDA expression. We functionally characterized the importance of ERF and WRKY binding sites for IDA expression during floral organ abscission. WRKY signaling is of special relevance to mediate IDA upregulation in response to biotic stress in tissues destined for separation. We further characterized WRKY57 as a potential positive regulator of IDA and IDA-like gene expression in abscission zones. Our findings highlight the promise of promoter element-targeted approaches to modulate the responsiveness of the IDA signaling pathway to harness abscission timing for improved crop productivity.

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Section: Resultsmentioning

confidence: 99%

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

Galindo-Trigo,

Bågman,

Ishida

et al. 2023

Preprint

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“…CO function is strongly regulated at both the transcriptional and post-translational levels in Arabidopsis , indicating that the photoperiodic pathway has been subjected to high selective pressure (Romero and Valverde, 2009)◻. Other than flowering transition and reproductive development, no other functions have been described for CO, but recent studies suggested that it could regulate floral jasmonate signaling (Serrano-Bueno et al, 2022) and circadian genes (Gnesutta et al, 2017)◻. Here, we show that CO alters the circadian clock by binding to the promoters of key clock-related genes, revealing an undescribed function.…”

Section: Discussionmentioning

confidence: 99%

“…CO encodes a protein with two conserved protein domains: two B-boxes located at the amino terminus, involved in protein-protein interactions, and a carboxyl CCT (CONSTANS, CO LIKE, TIMING OF CAB1) domain, which includes a nuclear transport signal and mediates interactions with DNA and diverse proteins (Laubinger et al, 2006; Valverde, 2011)◻. Recently, a role for the central part of CO in regulating floral senescence has been proposed (Serrano-Bueno et al, 2022). CO is a hub in the photoperiodic regulatory network; it receives signals from a multitude of different pathways, such as day length and circadian clock (Serrano-Bueno et al, 2021), and triggers the expression of the florigen FLOWERING LOCUS T ( FT ), which induces flower differentiation (Ahn et al, 2008)◻.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding other biological processes regulated by CO, it has been described that CO is able to modify starch metabolism through the activation of the GRANULE BOUND STARCH SYNTHASE ( GBSS ) gene (Ortiz-Marchena et al, 2014)◻. CO may also be involved in stomatal opening (Ando et al, 2013) and play a role in floral senescence (Serrano-Bueno et al, 2022)◻. However, the involvement of CO in other physiological processes is still largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Network theory constitutes one of the central paradigms in data integration and analysis in molecular systems biology (Barabási, 2015)◻. The integration of massive amounts of omics data has helped generate biological networks for photosynthetic organisms that reveal emergent properties and improve the understanding of processes such as the evolution of CO-like transcription factors (TFs) in Viridiplantae (Romero-Campero et al, 2013)◻, the conservation of circadian patterns across the plant phylogenetic tree (de los Reyes et al, 2017)◻, carbon mobilization during the floral transition in Arabidopsis (Ortiz-Marchena et al, 2014)□ and the involvement of hormonal control (Serrano-Bueno et al, 2022). In this work, we have constructed a transcriptional gene network by integrating deep sequencing of chromatin immunoprecipitation (ChIP) data generated from key TFs involved in the floral development/transition and circadian clock control.…”

Section: Introductionmentioning

confidence: 99%

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CONSTANS alters the circadian clock inArabidopsis thaliana

Reyes

Romero–Campero

Gao

et al. 2023

Preprint

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Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls the plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. CO expression is strongly regulated by the circadian clock. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily rhythmic movements in the leaves. We show that CO is able to activate key genes related to the circadian clock, such as CCA1, LHY, PRR5 and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a significant number of PRR5 repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, feeding seasonal information to the circadian system.

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ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis

Wu,

Wang,

Chen

et al. 2024

Physiol Mol Biol Plants

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Regulation of floral senescence in Arabidopsis by coordinated action of CONSTANS and jasmonate signaling

Cited by 20 publications

References 79 publications

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

CONSTANS alters the circadian clock inArabidopsis thaliana

ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis

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