Regulation of Gene Expression of Cancer/Testis Antigens in Colorectal Cancer Patients

Кутилин, Денис С.

doi:10.1134/s0026893320040093

Cited by 11 publications

(4 citation statements)

References 32 publications

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“…In the current work, we investigated the expression of 84 tumor-related miRNAs and used ML in order to identify miRNA signatures that seem to be associated with the response to irinotecan-based treatment in mCRC patients. Out of the top 20 miRNAs that were found to be differentially expressed in our samples, several of them have previously been linked with CRC [30][31][32]. Therefore, in line with previous observations, hsa-miR-155-5p [33,34], hsa-let-7i-5p [35], hsa-miR-92a-3p [36], hsa-miR-181d-5p [37], hsa-miR181b-5p [38], hsa-miR-10b-5p [39], hsa-let-7f-5p [40], and hsa-miR-181a-5p [41] were found to be upregulated in our cohort, whereas hsa-miR-215-5p [36,42], hsa-miR-143-3p [43,44], hsa-miR-148a-3p [45], hsa-miR-17-5p [46], hsa-miR-10a-5p [43], and hsa-let-7a-5p [47] were found to be downregulated.…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer

Pliakou

Lampropoulou²,

Dovrolis

et al. 2022

IJMS

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Colorectal cancer represents a leading cause of cancer-related morbidity and mortality. Despite improvements, chemotherapy remains the backbone of colorectal cancer treatment. The aim of this study is to investigate the variation of circulating microRNA expression profiles and the response to irinotecan-based treatment in metastatic colorectal cancer and to identify relevant target genes and molecular functions. Serum samples from 95 metastatic colorectal cancer patients were analyzed. The microRNA expression was tested with a NucleoSpin miRNA kit (Machnery-Nagel, Germany), and a machine learning approach was subsequently applied for microRNA profiling. The top 10 upregulated microRNAs in the non-responders group were hsa-miR-181b-5p, hsa-miR-10b-5p, hsa-let-7f-5p, hsa-miR-181a-5p, hsa-miR-181d-5p, hsa-miR-301a-3p, hsa-miR-92a-3p, hsa-miR-155-5p, hsa-miR-30c-5p, and hsa-let-7i-5p. Similarly, the top 10 downregulated microRNAs were hsa-let-7d-5p, hsa-let-7c-5p, hsa-miR-215-5p, hsa-miR-143-3p, hsa-let-7a-5p, hsa-miR-10a-5p, hsa-miR-142-5p, hsa-miR-148a-3p, hsa-miR-122-5p, and hsa-miR-17-5p. The upregulation of microRNAs in the miR-181 family and the downregulation of those in the let-7 family appear to be mostly involved with non-responsiveness to irinotecan-based treatment.

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Section: Discussionmentioning

confidence: 99%

Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer

Pliakou

Lampropoulou²,

Dovrolis

et al. 2022

IJMS

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“…In colorectal cancer, Kutilin observed that changes in the expression pattern of TAAs (eg, BAGE; SSX2 and PRAME1) was correlated with the activity of specific DNA methyltransferases (DNMT3A and DNMT3B). 37 Regulation of TAA-expression by such epigenetic modifications could represent important mechanisms of antigen-escape which are shared across different types of cancer. 38 The most relevant TAAs seem to differ between cancer types as observed in cross-cancer analyses using a proteomic approach.…”

Section: Discussionmentioning

confidence: 99%

Immune responses against shared antigens are common in esophago-gastric cancer and can be enhanced using CD40-activated B cells

Thelen

Keller²,

Lehmann³

et al. 2022

J Immunother Cancer

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BackgroundSpecific immune response is a hallmark of cancer immunotherapy and shared tumor-associated antigens (TAAs) are important targets. Recent advances using combined cellular therapy against multiple TAAs renewed the interest in this class of antigens. Our study aims to determine the role of TAAs in esophago-gastric adenocarcinoma (EGA).MethodsRNA expression was assessed by NanoString in tumor samples of 41 treatment-naïve EGA patients. Endogenous T cell and antibody responses against the 10 most relevant TAAs were determined by FluoroSpot and protein-bound bead assays. Digital image analysis was used to evaluate the correlation of TAAs and T-cell abundance. T-cell receptor sequencing, in vitro expansion with autologous CD40-activated B cells (CD40Bs) and in vitro cytotoxicity assays were applied to determine specific expansion, clonality and cytotoxic activity of expanded T cells.Results68.3% of patients expressed ≥5 TAAs simultaneously with coregulated clusters, which were similar to data from The Cancer Genome Atlas (n=505). Endogenous cellular or humoral responses against ≥1 TAA were detectable in 75.0% and 53.7% of patients, respectively. We found a correlation of T-cell abundance and the expression of TAAs and genes related to antigen presentation. TAA-specific T-cell responses were polyclonal, could be induced or enhanced using autologous CD40Bs and were cytotoxic in vitro. Despite the frequent expression of TAAs co-occurrence with immune responses was rare.ConclusionsWe identified the most relevant TAAs in EGA for monitoring of clinical trials and as therapeutic targets. Antigen-escape rather than missing immune response should be considered as mechanism underlying immunotherapy resistance of EGA.

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“…This protein is also expressed in normal testis tissue. MAGE-A4 also belongs to the CTA family of cancer/testis antigens [27][28][29]. Trim (tripartite motif ) proteins are a family of proteins with a wide range of biological activities in cells [30].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Serum Tumor-Associated Autoantibodies in Esophageal Cancer

Xiao

Wang

et al. 2021

Biomark. Med.

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Aim: To explore the application value of serum autoantibodies in the early diagnosis of esophageal cancer. Materials & methods: A total of 130 patients with esophageal cancer and 110 controls were included and tested by ELISA. Results: According to the receiver operating characteristic curve, total sensitivity is 83.08%, total specificity is 72.73%. A nomogram was established based on the positive judgment standard, the area under the receiver operating characteristic curve was calculated to be 0.880 after verification with the calibration curve. A 2-week follow-up analysis found compared with the preoperative control, the postoperative model integral value will significantly decrease. Conclusion: The combination of serum autoantibody groups has certain clinical application value in the early diagnosis of esophageal cancer and can be used as an auxiliary index for early diagnosis.

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Regulation of Gene Expression of Cancer/Testis Antigens in Colorectal Cancer Patients

Cited by 11 publications

References 32 publications

Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer

Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer

Immune responses against shared antigens are common in esophago-gastric cancer and can be enhanced using CD40-activated B cells

Diagnostic Value of Serum Tumor-Associated Autoantibodies in Esophageal Cancer

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