Regulation of gibberellin�20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Vidal, Antonio M.; Ben-Cheikh, W.; Talón, Manuel; Garcı́a-Martı́nez, José-Luis

doi:10.1007/s00425-003-0999-2

Cited by 49 publications

(26 citation statements)

References 20 publications

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“…The endogenous GAs found in citrus fruits are mainly members of the 13-hydroxylation pathway [GA 53 , GA 97 , GA 44 , GA 17 , GA 19 , GA 20 , GA 29 , GA 1 , epi-GA 1 , and GA 8 (Goto et al, 1989;Turnbull, 1989;Talon et al, 1990a] leading to GA 1 , the bioactive GA (Zeevaart et al, 1993). This pathway also operates in vegetative tissues of citrus (Vidal et al, 2001(Vidal et al, , 2003 where is thought to control shoot growth and elongation (Fagoaga et al, 2007). Developing fruits also contain at lower levels 3ß-and non-hydroxylated GAs, such as GA 4 , GA 24 , GA 25 , and GA 9 .…”

Section: Hormonal Regulators Of Fruit Growth and Abscissionmentioning

confidence: 99%

Physiology of citrus fruiting

Iglesias

Cercós

Colmenero‐Flores

et al. 2007

Braz. J. Plant Physiol.

310

232

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Citrus is the main fruit tree crop in the world and therefore has a tremendous economical, social and cultural impact in our society. In recent years, our knowledge on plant reproductive biology has increased considerably mostly because of the work developed in model plants. However, the information generated in these species cannot always be applied to citrus, predominantly because citrus is a perennial tree crop that exhibits a very peculiar and unusual reproductive biology. Regulation of fruit growth and development in citrus is an intricate phenomenon depending upon many internal and external factors that may operate both sequentially and simultaneously. The elements and mechanisms whereby endogenous and environmental stimuli affect fruit growth are being interpreted and this knowledge may help to provide tools that allow optimizing production and fruit with enhanced nutritional value, the ultimate goal of the Citrus Industry. This article will review the progress that has taken place in the physiology of citrus fruiting during recent years and present the current status of major research topics in this area. Key words: abiotic stresses, abscission, color break, flowering, fruit set, ripening Fisiologia da frutificação em citrus. Citrus é a principal fruteira no mundo, tendo, portanto, profundos impactos econômicos, sociais e culturais em nossa sociedade. Nos últimos anos, o conhecimento sobre a biologia reprodutiva de plantas tem aumentado consideravelmente, principalmente em função de trabalhos desenvolvidos com plantas-modelo. Todavia, a informação produzida nessas espécies nem sempre pode ser aplicada a citrus, fundamentalmente porque citrus é uma cultura arbórea perene com uma biologia reprodutiva muito peculiar e incomum. A regulação do crescimento e desenvolvimento do fruto em citrus é um fenômeno complexo e dependente de muitos fatores externos e internos que podem operar tanto seqüencialmente como simultaneamente. Os elementos e mecanismos pelos quais estímulos ambientes e endógenos afetam o crescimento do fruto vêm sendo interpretados, e esse conhecimento pode auxiliar a prover ferramentas que permitiriam otimizar a produção per se, além da obtenção de frutos com maior valor nutricional, o objetivo precípuo da Industria de Citrus. Neste artigo, revisam-se os avanços que vêm ocorrendo na fisiologia da frutificação de citrus durante os últimos anos; apresenta-se, também, o status atual de pesquisas mais relevantes nessa área. Palavras-chave: estresses abióticos, floração, maturação, vingamento de frutos

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Section: Hormonal Regulators Of Fruit Growth and Abscissionmentioning

confidence: 99%

Physiology of citrus fruiting

Iglesias

Cercós

Colmenero‐Flores

et al. 2007

Braz. J. Plant Physiol.

310

232

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“…On the other hand, genes such as NtGA20ox1 and NtGA3ox1 seem to be sensitive to high gibberellin levels, because their expression was altered only in slender transgenic plants with substantial gibberellin increases (20ox-OE and hybrid 3ox/20ox-OE). Interestingly, in citrus, it has also been described that GA20ox1 was negatively feedback regulated only in response to large changes in the active gibberellin content (Vidal et al 2003). The two different gene expression patterns found in response to gibberellin (one very sensitive to GA 1 for NtGA2ox3 and -5 and another mainly sensitive to high GA 1 concentrations for NtGA20ox1, NtGA3ox1 and NtGA2ox1; Fig.…”

Section: Regulation Of Gibberellin Metabolism Genesmentioning

confidence: 80%

Efecto de la sobreexpresión y del silenciamiento de genes del metabolismo de giberelinas sobre el desarrollo de tabaco

Giraldo¹,

Marcela²

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AGRADECIMIENTOSEste trabajo ha sido realizado gracias a una beca del Ministerio de Educación y Ciencia de España dentro del programa de becas de formación de personal investigador F.P.I.Quiero agradecer enérgicamente a todas las personas del Instituto de Biología Molecular y Celular de Plantas IBMCP, que han dedicado parte de su tiempo y de su conocimiento en enseñarme de que iba todo esto, pues gracias a ese cúmulo de información he podido realizar esta tesis doctoral. También quiero agradecer a las personas del instituto que desinteresadamente me han ofrecido una sonrisa y han compartido con migo su día a día.Gracias infinitas a mi familia, a mi hija Issabella por su paciencia y su compañía, al Dr. Juan Carlos Serrani, por su ayuda impecable prestada hasta último momento en este trabajo y a mi madre, a mi abuela y a mi hermana por no abandonarme nunca de pensamiento.

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“…(Eriksson & Moritz 2002), developing pumpkin seeds (Frisse et al 2003;Lange et al 2005), spinach (Lee & Zeevaart 2007), and in tomato (Serrani et al 2007). Regulation of gibberellin biosynthesis and gene expression have been investigated in Pisum sativum L (Martin et al 1996), tomato (Rebers et al 1999), potato (Carrera et al 2000), citrus (Vidal et al 2003), and in tobacco (Gallego-Giraldo et al 2008). An overview of gibberellin metabolism enzyme genes and their related mutants in rice has been reported (Kusaba et al 1998;).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

Dai

Cheng

et al. 2012

Biologia

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The gibberellin 20-oxidase gene (GA20) plays an important role in plant growth and development. Differential expression of Zoysiagrass (Zoysia japonica Steud.) gibberellin 20 oxidase gene (ZjGA20) induced by abiotic stresses has not been reported. In this investigation, we first reported the differential expression of ZjGA20 in different Z. japonica tissues including root, young leaf, senescent leaf, blade, sheath, and stolon, as well as differential expression induced by abiotic stresses including low temperature (4 • C), H2O2(8 µM), salt stress (250 mM NaCl), 25% PEG6000, and high temperature (42 • C) by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Higher expression of ZjGA20 was observed in young leaf and sheath, compared to root, senescent leaf, blade, and stolon. Among different abiotic stresses, expression of ZjGA20 decreased under low temperature, 25% PEG6000, and high temperature. The highest expression of ZjGA20 was obtained when plants were treated with 8 µM H2O2 for 10 h and with 250 mM NaCl for 5 h. The analysis of the MDA content, POD activity, and permeability of the plasma membrane demonstrated that application of exogenous GA3 recovered tissue damage derived from low temperature treatment. In addition, the expression of ZjGA20 increased under low temperature stress. These results demonstrated that expression of ZjGA20 was regulated by abiotic stresses and the damage derived from abiotic stresses could be rescued by exogenously applied plant growth regulator GA3. Further more, exogenous gibberellin and salicylic acid (SA) alleviated the growth inhibition and death of the seedlings under stresses. The SA content in the seedlings treated with 80 µM GA3 was far greater than the control (with H2O) and plants under stress treatments. These data suggest that exogenous addition of GA3 is able to counteract the inhibitory effects of these adverse environmental conditions in Zoysiagrass growth through modulation of SA biosynthesis. This is the first study of differential expression of gibberellin 20 oxidase gene and growth regulation of GA3 in Zoysiagrass under stresses.

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Regulation of gibberellin�20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Cited by 49 publications

References 20 publications

Physiology of citrus fruiting

Physiology of citrus fruiting

Efecto de la sobreexpresión y del silenciamiento de genes del metabolismo de giberelinas sobre el desarrollo de tabaco

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

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