Regulation of gibberellin on gene expressions related with the lignin biosynthesis in ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai) fruit

Yang, Shaolan; Zhang, Xianan; Lu, Guang Hong; Wang, Chengrong; Wang, Ran

doi:10.1007/s10725-014-9982-0

Cited by 34 publications

(20 citation statements)

References 29 publications

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“…Increases in GA contents stimulate lignin deposition under high irradiance conditions ( Wada et al, 2005 ; Kurepin et al, 2006 ; Moura et al, 2010 ; Bai et al, 2014 ; Yang et al, 2015 ; Hedden and Thomas, 2016 ; Li et al, 2017 ; Zhang et al, 2018a ). On the other hand, GA levels are higher under shaded conditions ( Kurepin et al, 2006 ; Kurepin and Pharis, 2014 ), which in turn triggers cell stretching and etiolation, with lower lignin deposition over all secondary xylem (SX) fiber-like cells.…”

Section: Introductionmentioning

confidence: 99%

Increased Gibberellins and Light Levels Promotes Cell Wall Thickness and Enhance Lignin Deposition in Xylem Fibers

et al. 2018

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Light intensity and hormones (gibberellins; GAs) alter plant growth and development. A fine regulation triggered by light and GAs induces changes in stem cell walls (CW). Cross-talk between light-stimulated and GAs-induced processes as well as the phenolic compounds metabolism leads to modifications in lignin formation and deposition on cell walls. How these factors (light and GAs) promote changes in lignin content and composition. In addition, structural changes were evaluated in the stem anatomy of tobacco plants. GA3 was sprayed onto the leaves and paclobutrazol (PAC), a GA biosynthesis inhibitor, via soil, at different irradiance levels. Fluorescence microscopy techniques were applied to detect lignin, and electron microscopy (SEM and TEM) was used to obtain details on cell wall structure. Furthermore, determination of total lignin and monomer contents were analyzed. Both light and GAs induces increased lignin content and CW thickening as well as greater number of fiber-like cells but not tracheary elements. The assays demonstrate that light exerts a role in lignification under GA3 supplementation. In addition, the existence of an exclusive response mechanism to light was detected, that GAs are not able to replace.

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Section: Introductionmentioning

confidence: 99%

Increased Gibberellins and Light Levels Promotes Cell Wall Thickness and Enhance Lignin Deposition in Xylem Fibers

et al. 2018

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“…The upstream pathway of lignin metabolism is the general phenylpropanoid pathway [ 16 ], which mainly involves three enzymes: phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and 4-hydroxycinnamate-CoA ligase (4CL). These enzymes convert L-phenylalanine (L-Phe) to p-coumaroyl-CoA [ 10 , 12 ]. Then, p-coumaroyl-CoA enters into the ester intermediary pathway, which results in the formation of various hydroxycinnamic acids and coenzyme A-thioesters by HCT, C3H and cafeoyl-CoA O-methyltransferase (CCoAOMT) [ 12 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents

et al. 2017

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Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related to carbon metabolism, such as sorbitol dehydrogenase-like (SDH-like) and ATP-dependent 6-phosphofructokinase (ATP-PFK). At the peak of the stone cell content (55 days after flowering), the expression level of these genes in 'Dangshan Su' was significantly increased compared with 'Lianglizaosu', indicating that these genes were closely related to stone cell development. We validated the transcriptional levels of 33 DEGs using quantitative realtime polymerase chain reaction (qRT-PCR) analysis. The results were consistent with the transcriptome analysis, indicating the reliability of transcriptome data. In addition, subcellular localization analysis of three DEGs in lignin synthesis (PbC3H, PbF5H and PbPOD) revealed that these proteins are mainly distributed in the cell membrane and cytoplasm. These results provide new insights into the molecular mechanism of stone cell formation.

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“…Lignin accumulation in perennial woody trees but rarely occurs in flesh fruit and has mainly been reported in pear (stone cells, Lu et al, 2015 ; Yang et al, 2015 ), mangosteen (pericarp, Ketsa and Atantee, 1998 ; Kamdee et al, 2014 ), and loquat ( Cai et al, 2006c ). In loquat fruit, lignin content increasing during postharvest storage in flesh (edible layer), which was associated with an increase in firmness and reduction of juice yield ( Cai et al, 2006b ), subsequently adversely affecting fruit edible quality.…”

Section: Introductionmentioning

confidence: 99%

EjODO1, a MYB Transcription Factor, Regulating Lignin Biosynthesis in Developing Loquat (Eriobotrya japonica) Fruit

Zhang¹,

Zang

et al. 2016

Front. Plant Sci.

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Lignin is important for plant secondary cell wall formation and participates in resistance to various biotic and abiotic stresses. Loquat undergoes lignification not only in vegetative tissues but also in flesh of postharvest fruit, which adversely affects consumer acceptance. Thus, researches on lignin biosynthesis and regulation are important to understand loquat fruit lignification. In loquat, a gene encoding an enzyme in the lignin biosynthesis pathway, Ej4CL1, was reported to be regulated by transcription factors, including EjMYB1, EjMYB2, EjMYB8, and EjAP2-1, knowledge of this process is still limited. With the aim of identifying novel transcriptional factors controlling lignin biosynthesis in loquat, the promoter of Ej4CL1 was utilized to screen a cDNA library by yeast one hybrid assay. A novel R2R3 MYB, named EjODO1, was identified. Real-time PCR analyses indicated that EjODO1 is highly expressed in lignified stems and roots. During fruit development, expression of EjODO1 decreased along with the reduction of lignin content and became undetectable in mature ripe fruit. Thus, EjODO1 is likely to be involved in lignification of vegetative organs and early fruit development but not in mature fruit or postharvest lignification. Dual-luciferase assay indicated that EjODO1 could trans-activate promoters of lignin biosynthesis genes, such as EjPAL1, Ej4CL1, and Ej4CL5 and transient overexpression of EjODO1 triggered lignin biosynthesis. These results indicate a role for EjODO1 in regulating lignin biosynthesis in loquat which is different from the previously characterized transcription factors.

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Regulation of gibberellin on gene expressions related with the lignin biosynthesis in ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai) fruit

Abstract: Wangkumbae' pear (Pyrus pyrifolia Nakai)

Cited by 34 publications

References 29 publications

Increased Gibberellins and Light Levels Promotes Cell Wall Thickness and Enhance Lignin Deposition in Xylem Fibers

Increased Gibberellins and Light Levels Promotes Cell Wall Thickness and Enhance Lignin Deposition in Xylem Fibers

Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents

EjODO1, a MYB Transcription Factor, Regulating Lignin Biosynthesis in Developing Loquat (Eriobotrya japonica) Fruit

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