Regulation of Glucose Transport by Tumor Necrosis Factor-α in Cultured Murine 3T3-L1 Fibroblasts

Cornelius, Peter; Marlowe, Melissa; Pekala, Phillip H.

doi:10.1097/00005373-199012001-00006

Cited by 24 publications

(10 citation statements)

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“…In these situations TNF‐α induces lipolysis, catabolism, and insulin resistance [21–23]. On the other hand, TNF‐α increased glucose transport and utilization in several transformed, as well as normal, cell lines [24–27]. We found that TNF‐α induced a dose dependent remarkable elevation of glucose consumption in MCF‐7 human breast cancer cells.…”

Section: Resultsmentioning

confidence: 68%

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

Kaplan

Ruı́z-Cabello²,

Cohen

1997

FEBS Letters

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Tumor necrosis factor‐α inhibited growth of cultured MCF‐7 human breast cancer cells in a dose dependent manner. Tumor necrosis factor‐α also markedly increased glucose consumption, and its cytotoxicity was modified by glucose concentrations in the growth medium; higher glucose levels were associated with increased cell survival. However, when the cells were perfused in physiological conditions, very high levels of tumor necrosis factor‐α (200 ng/ml) in the perfusion solution had no inhibitory effects. Moreover, tumor necrosis factor‐α had no effects on 31P nuclear magnetic resonance spectra of the perfused cells. In the traditional growth inhibition assays, cells are incubated for several days with a drug, a situation where their metabolism is altered due to the depletion of nutrients, the accumulation of toxic waste materials and pH changes. Perfusion experiments are more relevant to in vivo conditions, and may be used for studying metabolic processes and the mechanisms of action of therapeutic agents.

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Section: Resultsmentioning

confidence: 68%

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

Kaplan

Ruı́z-Cabello²,

Cohen

1997

FEBS Letters

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“…The activated immune cells secrete pro-inflammatory cytokines (tumour necrosis factor-a, interleukin-17 etc.) which significantly upregulate glucose metabolism in fibroblasts and activated macrophages 2,3 and contribute to the formation of bone-resorbing "pannus", a hallmark feature of the diseases. 4 The positron emission tomography (PET) radiotracer fluorine-18 fludeoxyglucose ( 18 F-FDG)-a glucose analogue-is taken up by cells with elevated glucose utilization and is therefore particularly suited for imaging cells mounting an immune response in inflammatory arthritis.…”

Section: Introductionmentioning

confidence: 99%

High-resolution¹⁸F-FDG PET/CT for assessing disease activity in rheumatoid and psoriatic arthritis: findings of a prospective pilot study

Chaudhari¹,

Ferrero²,

Godinez³

et al. 2016

BJR

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Objective: Rheumatoid arthritis (RA) and psoriatic arthritis (PsA) commonly affect the small joints of the wrist and hand. We evaluated the performance of a new, highresolution extremity positron emission tomography (PET)/CT scanner for characterizing and quantifying pathologies associated with the two arthritides in the wrist and hand joints. Methods: Patients with RA or PsA underwent fluorine-18 fludeoxyglucose ( 18 F-FDG) PET/CT wrist and hand imaging, respectively, on the high-resolution scanner. Calibrated CT images and co-registered PET images were reconstructed. PET/CT was derived for the radiocarpal and pisiform-triquetral compartments, joints with erosive changes, sites of synovitis or tenosynovitis and the nail bed and were correlated with clinical and MRI findings.

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“…This offers the opportunity of a quantifiable 18 F-FDG PET signal from sites of pathological increased glucose metabolism in the tissue (that is, sites of inflammation). In addition, glucose metabolism is affected by proinflammatory tumor necrosis factor-alpha (TNF-α) and characteristically increased in inflamed tissue [1,2], making PET a potentially interesting technique for the detection and quantification of inflammation. Analyses of radioisotope-labelled FDG uptake in vitro revealed a distinct accumulation in fibroblasts and neutrophils, whereas resting macrophages incorporated only small amounts [3].…”

Section: Introductionmentioning

confidence: 99%

In vivo molecular imaging of experimental joint inflammation by combined 18F-FDG positron emission tomography and computed tomography

et al. 2010

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IntroductionThe purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation.MethodsSignalling of radioisotope 18F labelled Fluorodeoxyglucose (18F-FDG) injected in mice with glucose-6-phosphate isomerase (G6PI)-induced arthritis was analysed by PET/CT. Accumulation of 18F-FDG in tissue was quantified by PET measurement, whereas high definition CT delivered anatomical information. The fusion of both images revealed in detail spatial and temporal distribution and metabolism of 18F-FDG.ResultsA distinct 18F-FDG signal could be measured by PET in carpal and tarsal joints, from mice with early or established arthritis. In contrast, no accumulation of 18F-FDG was detectable before arthritis onset. Comparison of 18F-FDG joint uptake with histopathological evaluation revealed a significant correlation of both methods.ConclusionsSmall animal PET/CT using 18F-FDG is a feasible method for monitoring and, more importantly, quantitative assessment of inflammation in G6PI-arthritis. Since it is possible to perform repeated non-invasive measurements in vivo, not only numbers of animals in preclinical studies can markedly be reduced by this method, but also longitudinal studies come into reach, e. g. for individual flare-up reactions or monitoring therapy response in progressive arthritis.

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Regulation of Glucose Transport by Tumor Necrosis Factor-α in Cultured Murine 3T3-L1 Fibroblasts

Cited by 24 publications

References 0 publications

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

High-resolution¹⁸F-FDG PET/CT for assessing disease activity in rheumatoid and psoriatic arthritis: findings of a prospective pilot study

In vivo molecular imaging of experimental joint inflammation by combined 18F-FDG positron emission tomography and computed tomography

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Regulation of Glucose Transport by Tumor Necrosis Factor-α in Cultured Murine 3T3-L1 Fibroblasts

Cited by 24 publications

References 0 publications

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption

High-resolution18F-FDG PET/CT for assessing disease activity in rheumatoid and psoriatic arthritis: findings of a prospective pilot study

In vivo molecular imaging of experimental joint inflammation by combined 18F-FDG positron emission tomography and computed tomography

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High-resolution¹⁸F-FDG PET/CT for assessing disease activity in rheumatoid and psoriatic arthritis: findings of a prospective pilot study