Regulation of Glutathione S-Transferase Mu with type 1 diabetes and its regulation with antioxidants

Sadı, Gökhan; Kartal, Deniz İrtem; Güray, Tülïn

doi:10.5505/tjb.2013.96720

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…In a study conducted on STZ-induced type 1 diabetic rats, GST activity was found to decrease. GST mRNA is diminished due to oxidation of various transcription factors essential for antioxidant enzyme transcription or may be due to destabilization of mRNA owing to its decreased half-lives because of increased oxidative stress [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Role of Pro-Inflammatory Cytokines and Biochemical Markers in the Pathogenesis of Type 1 Diabetes: Correlation with Age and Glycemic Condition in Diabetic Human Subjects

et al. 2016

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BackgroundType 1 diabetes mellitus is a chronic inflammatory disease involving insulin producing β-cells destroyed by the conjoined action of auto reactive T-cells, inflammatory cytokines and monocytic cells. The aim of this study was to elucidate the status of pro-inflammatory cytokines and biochemical markers and possible correlation of these factors towards outcome of the disease.MethodsThe study was carried out on 29 T1D subjects and 20 healthy subjects. Plasma levels of oxidative stress markers, enzymatic and non-enzymatic antioxidants were estimated employing biochemical assays. The levels of pro-inflammatory cytokines such as by IL-1β & IL-17 in the serum were determined by ELISA, while the expression of TNF-α, IL-23 & IFN-γ was ascertained by qRT-PCR.ResultsThe onset of T1D disease was accompanied with elevation in levels of Plasma malondialdehyde, protein carbonyl content and nitric oxide while plasma vitamin C, reduced glutathione and erythrocyte sulfhydryl groups were found to be significantly decreased in T1D patients as compared to healthy control subjects. Activity of antioxidant enzymes, superoxide dismutase, catalase, glutathione reductase and glutathione-s-transferase showed a significant suppression in the erythrocytes of T1D patients as compared to healthy subjects. Nevertheless, the levels of pro-inflammatory cytokines IL-1β and IL-17A were significantly augmented (***p≤.001) on one hand, while expression of T cell based cytokines IFN-γ, TNF-α and IL-23 was also up-regulated (*p≤.05) as compared to healthy human subjects.ConclusionThe level of pro-inflammatory cytokines and specific biochemical markers in the serum of the patient can be exploited as potential markers for type 1 diabetes pathogenesis. The study suggests that level of inflammatory markers is up-regulated in T1D patients in an age dependent manner.

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Section: Discussionmentioning

confidence: 99%

Role of Pro-Inflammatory Cytokines and Biochemical Markers in the Pathogenesis of Type 1 Diabetes: Correlation with Age and Glycemic Condition in Diabetic Human Subjects

et al. 2016

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“…Another protective mechanism against oxidative stress is glutathione S-transferases (GSTs), which catalyze the conjugation of glutathione to a wide range of electrophiles. They exhibit differential response to a wide variety of chemicals and oxidative stress in the normal and pathophysiological conditions such as diabetes mellitus [ 25 ]. Among the different isoenzymes of GSTs, the cytosolic Mu and Pi isoenzymes function in the elimination of diverse array of foreign compounds as well as wide variety of products of oxidative stress [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…For the determination of SOD-1, SOD-2 and GST-Mu protein contents, whole homogenates containing 10 μg (20 μg for GST-Mu) of proteins were separated by SDS-PAGE and electroblotted onto PVDF membranes [ 23 ]. Blotted membranes were then blocked with 5% (w/v) bovine serum albumin and incubated with prevalidated [ 24 , 25 ] primary antibodies; SOD-1 (Anti-SOD-1 Sheep IgG, Calbiochem- 574597, Billerica, USA, 1:5000), SOD-2 (Anti-SOD-2 Rabbit IgG, Santa Cruz- sc-30080, USA, 1:100), GST-Mu (Anti-GST-Mu Rabbit IgG, Abcam- ab77925, Cambridge, USA, 1:6000) for two hours. As an internal control, GAPDH proteins were also labeled with anti-GAPDH Rabbit IgG (Santa Cruz-sc-25778, USA, 1:2000) for the normalization.…”

Section: Methodsmentioning

confidence: 99%

Differential Gene Expression in Liver Tissues of Streptozotocin-Induced Diabetic Rats in Response to Resveratrol Treatment

2015

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This study was conducted to elucidate the genome-wide gene expression profile in streptozotocin induced diabetic rat liver tissues in response to resveratrol treatment and to establish differentially expressed transcription regulation networks with microarray technology. In addition to measure the expression levels of several antioxidant and detoxification genes, real-time quantitative polymerase chain reaction (qRT-PCR) was also used to verify the microarray results. Moreover, gene and protein expressions as well as enzymatic activities of main antioxidant enzymes; superoxide dismutase (SOD-1 and SOD-2) and glutathione S-transferase (GST-Mu) were analyzed. Diabetes altered 273 genes significantly and 90 of which were categorized functionally which suggested that genes in cellular catalytic activities, oxidation-reduction reactions, co-enzyme binding and terpenoid biosynthesis were dominated by up-regulated expression in diabetes. Whereas; genes responsible from cellular carbohydrate metabolism, regulation of transcription, cell signal transduction, calcium independent cell-to-cell adhesion and lipid catabolism were down-regulated. Resveratrol increased the expression of 186 and decreased the expression of 494 genes in control groups. While cellular and extracellular components, positive regulation of biological processes, biological response to stress and biotic stimulants, and immune response genes were up-regulated, genes responsible from proteins present in nucleus and nucleolus were mainly down-regulated. The enzyme assays showed a significant decrease in diabetic SOD-1 and GST-Mu activities. The qRT-PCR and Western-blot results demonstrated that decrease in activity is regulated at gene expression level as both mRNA and protein expressions were also suppressed. Resveratrol treatment normalized the GST activities towards the control values reflecting a post-translational effect. As a conclusion, global gene expression in the liver tissues is affected by streptozotocin induced diabetes in several specific pathways. The present data suggest the presence of several processes which contribute and possibly interact to impair liver functions in type 1 diabetes, several of which are potentially amenable to therapeutic interventions with resveratrol.

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“…It is also known that hyperglycemia is caused by non-internalization of glucose in the cells of diabetic patients, which contributes to increased ROS generation and oxidative stress which can damage the bases of DNA (LazaldeRamos et al, 2012). Sadi et al (2013) observed that diabetes causes a significant decrease in the mRNA expression of GSTM1. It is known that the metabolic processes including glucose oxidation, enzymatic glycation of proteins, and subsequent oxidative degradation of glycated proteins can be produce ROS in diabetic patients, and these changes subsequently reduce antioxidant defense mechanisms, simultaneously leading to lipid peroxidation and damage to cells.…”

Section: Discussionmentioning

confidence: 94%

“…Thus, excessive oxidative stress occurring in the liver due to diabetes mellitus was suggested to result in the down-regulation of GST mu, superoxide dismutase, and catalase gene expression. Accordingly, decreased mRNA expression of the GST mu isoenzyme in patients with diabetes could be due to a decreased half-life of its mRNA because increased oxidative stress might lead to destabilization of mRNA (Sadi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Association of the GSTM1 null polymorphism with breast cancer in a Mexican population

Soto-Quintana¹,

Zúñiga‐González²,

Ramírez-Patiño³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The glutathione S transferase (GST) family plays an important role in the processing of carcinogens. Data on the null GSTM1 genotype has revealed associations with cancer, and has been suggested to affect carcinogen metabolism and to contribute to tumor promotion in 13067 GSTM1 polymorphism in breast cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 13066-13075 (2015) the mammary gland. We examined the role of the null GSTM1 genotype by comparing the genotypes of 276 healthy Mexican women with those of 558 Mexican women with breast cancer (BC). The genotype frequencies observed in the controls and patients with BC were 38 and 45% for the null GSTM1 genotype, respectively. The obtained odds ratio (OR) was 1.36, with a 95% confidence interval (95%CI) of 1.02-1.8, P = 0.04. The protective association was also evident upon analysis of the distributions of the null GSTM1 genotype in patients with positive chemotherapy response who had high plasma levels of glucose (OR 0.56, 95%CI = 0.33-0.94, P = 0.03). This study suggested that the null GSTM1 genotype is associated with BC susceptibility in the Mexican population analyzed.

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Regulation of Glutathione S-Transferase Mu with type 1 diabetes and its regulation with antioxidants

Cited by 7 publications

References 31 publications

Role of Pro-Inflammatory Cytokines and Biochemical Markers in the Pathogenesis of Type 1 Diabetes: Correlation with Age and Glycemic Condition in Diabetic Human Subjects

Role of Pro-Inflammatory Cytokines and Biochemical Markers in the Pathogenesis of Type 1 Diabetes: Correlation with Age and Glycemic Condition in Diabetic Human Subjects

Differential Gene Expression in Liver Tissues of Streptozotocin-Induced Diabetic Rats in Response to Resveratrol Treatment

Association of the GSTM1 null polymorphism with breast cancer in a Mexican population

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