Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator

Cupertino, Fernanda Barbosa; Virgílio, Stela; Freitas, Fernanda Zanolli; Candido, Thiago de Souza; Bertolini, Maria Célia

doi:10.1016/j.fgb.2015.03.011

Cited by 28 publications

(28 citation statements)

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“…When GFP‐CreA‐expressing strain was incubated in 1% xylose medium for 40 min, GFP fluorescence was mainly observed in the cytoplasm, although some weak GFP fluorescence was observed in the nucleus. These results demonstrate that CreA is exported from nuclei to the cytoplasm under conditions that induce the production of secretory hydrolytic enzymes, as reported in other filamentous fungi (Brown et al, ; Lichius et al, ; Cupertino et al, ). However, GFP fluorescence of C‐terminally GFP‐fused CreA (CreA‐GFP) was still observed in the nucleus in over 70% of the hyphae even 40 min after incubation in 1% maltose medium (Fig.…”

Section: Resultssupporting

confidence: 84%

“…In the presence of high levels of glucose, Mig1 is dephosphorylated and retained in the nucleus. The authors of recent studies have reported that subcellular localization change of CreA and its counterparts is also observed in filamentous fungi such as Aspergillus nidulans , Trichoderma reesei and Neurospora crassa (Brown et al, ; Lichius et al, ; Cupertino et al, ). In these reports, glucose induced the nuclear import of CreA, and the nuclear accumulation of CreA was lost by substrates that induced hydrolytic enzymes.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear export‐dependent degradation of the carbon catabolite repressor CreA is regulated by a region located near the C‐terminus in Aspergillus oryzae

Tanaka

Ichinose

Shintani

et al. 2018

Molecular Microbiology

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Carbon catabolite repression (CCR) is regulated by the C H -type transcription factor CreA/Cre1 in filamentous fungi including Aspergillus oryzae. We investigated the stability and subcellular localization of CreA in A. oryzae. The abundance of FLAG-tagged CreA (FLAG-CreA) was dramatically reduced after incubation in maltose and xylose, which stimulated the export of CreA from the nucleus to the cytoplasm. Mutation of a putative nuclear export signal resulted in nuclear retention and significant stabilization of CreA. These results suggest that CreA is rapidly degraded in the cytoplasm after export from the nucleus. The FLAG-CreA protein level was reduced by disruption of creB and creC, which encode the deubiquitinating enzyme complex involved in CCR. In contrast, FLAG-CreA stability was not affected by disruption of creD which encodes an arrestin-like protein required for CCR relief. Deletion of the last 40 C-terminal amino acids resulted in remarkable stabilization and increased abundance of FLAG-CreA, whereas deletion of the last 20 C-terminal amino acids had no apparent effect on CreA stability. This result suggests that the 20 amino acid region located between positions 390 and 409 of CreA is critical for the rapid degradation of CreA.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Nuclear export‐dependent degradation of the carbon catabolite repressor CreA is regulated by a region located near the C‐terminus in Aspergillus oryzae

Tanaka

Ichinose

Shintani

et al. 2018

Molecular Microbiology

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“…Recent work has identified other factors impacting cross-talk between metabolic state and circadian transcription (e.g., RCO-1 and RCM-1) and metabolic genes (e.g., CRE-1) (Cupertino et al, 2015; Olivares-Yañez et al, 2016). CRE-1 (NCU08807) was significantly rhythmic at the RNA and protein (p = 0.0013) level, demonstrating a metabolic-responsive transcription factor also regulated by the clock.…”

Section: Discussionmentioning

confidence: 99%

Circadian Proteomic Analysis Uncovers Mechanisms of Post-Transcriptional Regulation in Metabolic Pathways

et al. 2018

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SUMMARY Transcriptional and translational feedback loops in fungi and animals drive circadian rhythms in transcript levels that provide output from the clock, but post-transcriptional mechanisms also contribute. To determine the extent and underlying source of this regulation, we applied newly developed analytical tools to a long-duration, deeply sampled, circadian proteomics time course comprising half of the proteome. We found a quarter of expressed proteins are clock regulated, but >40% of these do not arise from clock-regulated transcripts, and our analysis predicts that these protein rhythms arise from oscillations in translational rates. Our data highlighted the impact of the clock on metabolic regulation, with central carbon metabolism reflecting both transcriptional and post-transcriptional control and opposing metabolic pathways showing peak activities at different times of day. The transcription factor CSP-1 plays a role in this metabolic regulation, contributing to the rhythmicity and phase of clock-regulated proteins.

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“…The regulation of CreA/CRE1/CRE-1 appears to be at least partially dependent on intracellular localization. Under repressing conditions, CreA/CRE1/CRE-1 is found in the nucleus where it can regulate the expression of target genes, and when under de-repressing conditions, CreA/CRE1/CRE-1 exits the nucleus (Brown et al, 2013;Cupertino et al, 2015;Lichius et al, 2014). However, the genetic regulation of CreA/CRE1/CRE-1 appears to be fairly divergent amongst the filamentous fungi.…”

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confidence: 99%

Regulation of the lignocellulolytic response in filamentous fungi

Huberman

Liu

Qin

et al. 2016

Fungal Biology Reviews

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Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator

Cited by 28 publications

References 51 publications

Nuclear export‐dependent degradation of the carbon catabolite repressor CreA is regulated by a region located near the C‐terminus in Aspergillus oryzae

Nuclear export‐dependent degradation of the carbon catabolite repressor CreA is regulated by a region located near the C‐terminus in Aspergillus oryzae

Circadian Proteomic Analysis Uncovers Mechanisms of Post-Transcriptional Regulation in Metabolic Pathways

Regulation of the lignocellulolytic response in filamentous fungi

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