Regulation of Granulosa Cell Proliferation: Facilitative Roles of Platelet-Derived Growth Factor and Low Density Lipoprotein*

May, Jeffrey V.; Frost, Joan P.; Bridge, Alisa J.

doi:10.1210/endo-126-6-2896

Cited by 24 publications

(12 citation statements)

References 42 publications

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“…Similar observations were made in three separate experiments. lated proliferation is enhanced by PDGF (42)(43)(44). Interestingly, a synergistic action between EGF and PDGF is not limited to mitogenesis, i.e.…”

Section: Discussionmentioning

confidence: 99%

Platelet-derived Growth Factor-stimulated Migration of Murine Fibroblasts Is Associated with Epidermal Growth Factor Receptor Expression and Tyrosine Phosphorylation

Kim

Bertics

2000

Journal of Biological Chemistry

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Previous studies have shown that epidermal growth factor (EGF) synergizes with various extracellular matrix components in promoting the migration of B82L fibroblasts expressing wild-type EGF receptors and that functional EGF receptors are critical for the conversion of B82L fibroblasts to a migratory cell type (1). In the present study, we examined the effects of platelet-derived growth factor (PDGF) on the motility of B82L fibroblasts using a microchemotaxis chamber. We found that PDGF can enhance fibronectin-induced migration of B82L fibroblasts expressing wild-type EGF receptors (B82L-clone B3). However, B82L cells that lack the EGF receptor (B82L-parental) or that express an EGF receptor that is kinase-inactive (B82L-K721M) or C-terminally truncated (B82L-c973) exhibit little PDGF-stimulated migration. In addition, none of these three cell lines exhibit the capacity to migrate to fibronectin alone. These observations indicate that, similar to cell migration toward fibronectin, PDGF-induced cell migration of B82L fibroblasts is augmented by the expression of an intact EGF receptor kinase. The loss of PDGF-stimulated motility in B82L cells that do not express an intact EGF receptor does not appear to result from a gross dysfunction of PDGF receptors, because ligand-stimulated tyrosine phosphorylation of the PDGF-␤ receptor and the activation of mitogen-activated protein kinases are readily detectable in these cells. Moreover, an interaction between EGF and PDGF receptor systems is supported by the observation that the EGF receptor exhibits an increase in phosphotyrosine content in a timedependent fashion upon the addition of PDGF.Altogether, these studies demonstrate that the expression of EGF receptor is critical for PDGF-stimulated migration of murine B82L fibroblasts and suggest a role for the EGF receptor downstream of PDGF receptor activation in the signaling events that lead to PDGFstimulated cell motility.

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Section: Discussionmentioning

confidence: 99%

Platelet-derived Growth Factor-stimulated Migration of Murine Fibroblasts Is Associated with Epidermal Growth Factor Receptor Expression and Tyrosine Phosphorylation

Kim

Bertics

2000

Journal of Biological Chemistry

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“…It is unknown at this time why the response to FSH was not significant in the present studies, given that it is generally accepted that gonadotropin treatment is necessary if the full effects of IGF-1 responsiveness are to be observed [1,39]. In the present studies, IGF-1 anahogs were more potent than IGF-1 at stimulation of estradiol production by tightly bound granulosa cells (experiment 1 and 48 h postweaning, experiment 2) and in the stimulation of progesterone production by tightly bound and weakly associated cells (both experiments).…”

mentioning

confidence: 90%

Differential Steroidogenic Response of Subpopulations of Porcine Granulosa Cells to Insulin-Like Growth Factor-1 (IGF-1) or IGF-1 Analogs1

Howard

Ford

1994

Biology of Reproduction

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Two experiments were conducted to examine responses of porcine granulosa cells to insulin-like growth factor-1 (IGF-1) or IGF-1 analogs (des [1-3] and Long R3) that have reduced affinity for IGF-binding proteins (IGFBP). Both experiments evaluated estradiol and IGFBP production by granulosa cells after separation of cells into subpopulations that maintain long-term estradiol production in vitro (tightly bound) and those that do not (weakly associated). Granulosa cells were obtained from medium-sized follicles (4-6 mm) at random stages of the estrous cycle in experiment 1 and from the 10 largest follicles per ovary at 0 or 48 h after weaning in experiment 2. Follicle diameter and follicular fluid estradiol concentrations increased with time after weaning (p < 0.05). Tightly bound cells produced more estradiol than weakly associated cells at 24-120 h of culture in experiment 1 and from 0 to 48 h in experiment 2 (p < 0.05). In tightly bound but not weakly associated cells, IGF-1 stimulated estradiol production. The IGF analogs were more potent stimulators than IGF-1 in experiment 1 (p < 0.05); and in experiment 2, this response was restricted to cells collected at 48 h after weaning. Conversely, tightly bound cells obtained at 0 h after weaning responded similarly to IGF-1 and des (1-3). During the final 48 h of culture, weakly associated cells produced greater quantities of 28-30-kDa IGFBP than did tightly bound cells in response to IGF-1 or analogs (both experiments; p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Granulosa cells synthesize insulin-like growth factor-I (IGF-I), a potent promoter of ovarian cellular differentiation and proliferation [9]. IGF-I enhances lipoprotein-stimulated progesterone production by porcine granulosa cells [10] and facilitates IGF-I-stimulated bovine and porcine granulosa cell proliferation [9,11]. To our knowledge, the reciprocal effects of lipoproteins on IGF-I production and cellular viability/proliferation of bovine granulosa and theca cells have not been examined.…”

Section: Introductionmentioning

confidence: 99%

Steroidogenic Activity, Insulin-Like Growth Factor-I Production, and Proliferation of Granulosa and Theca Cells Obtained from Dominant Preovulatory and Nonovulatory Follicles during the Bovine Estrous Cycle: Effects of Low-Density and High-Density Lipoproteins1

Bao

Thomas

Griffith

et al. 1995

Biology of Reproduction

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The role of high- and low-density lipoproteins (HDL and LDL) in regulating steroidogenic activity, cellular viability and proliferation, and insulin-like growth factor-I (IGF-I) production was examined in granulosa and theca cells from dominant preovulatory (DO) and nonovulatory (DNO) bovine follicles. Follicles were obtained from pluriparous nonlactating beef cows at ovariectomy 24 h after administration of a luteolytic dose of prostaglandin F2 alpha (DO) or during the first follicular wave on Days 5-8 of the estrous cycle (DNO). Lipoprotein effects on hormone production (ng/1.5 x 10(5) viable cells) and cell viability were studied in serum-free, defined medium containing LH and FSH (granulosa) or LH only (theca). During late stages (96-144 h) of culture, HDL in the presence of gonadotropins increased (p < 0.001) the production of progesterone by granulosa and theca cells and the production of IGF-I by granulosa cells. LDL did not stimulate granulosa or thecal progesterone synthesis and attenuated HDL-stimulated progesterone production by both cell types. Gonadotropin stimulation of terminal synthetic pathways was either attenuated (granulosa estradiol production) by addition of lipoproteins or maximally stimulated (theca cell androstenedione production) by a combination of LDL and HDL. Both lipoproteins increased (p < 0.05) granulosa cell viability in both follicle types, and a marked proliferation (p < 0.001) of steroidogenically inactive theca cells was observed from DO but not DNO follicles. Proliferation potential appeared to be switched off during the late stages of maturation of DNO follicles and switched on after induced luteal regression and rescue of DO follicles.

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Regulation of Granulosa Cell Proliferation: Facilitative Roles of Platelet-Derived Growth Factor and Low Density Lipoprotein*

Cited by 24 publications

References 42 publications

Platelet-derived Growth Factor-stimulated Migration of Murine Fibroblasts Is Associated with Epidermal Growth Factor Receptor Expression and Tyrosine Phosphorylation

Platelet-derived Growth Factor-stimulated Migration of Murine Fibroblasts Is Associated with Epidermal Growth Factor Receptor Expression and Tyrosine Phosphorylation

Differential Steroidogenic Response of Subpopulations of Porcine Granulosa Cells to Insulin-Like Growth Factor-1 (IGF-1) or IGF-1 Analogs1

Steroidogenic Activity, Insulin-Like Growth Factor-I Production, and Proliferation of Granulosa and Theca Cells Obtained from Dominant Preovulatory and Nonovulatory Follicles during the Bovine Estrous Cycle: Effects of Low-Density and High-Density Lipoproteins1

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