Regulation of GSK-3β and β-Catenin by Gαq in HEK293T cells

Salmanian, Sara; Najafi, Seyed Mahmoud Arab; Rafipour, Maryam; Arjomand, Maryam Rezaei; Shahheydari, Hamideh; Ansari, Sara; Kashkooli, Leily; Rasouli, S. Javad; Jazi, Marie Saghaeian; Minaei, Tayebeh

doi:10.1016/j.bbrc.2010.04.087

Cited by 20 publications

(34 citation statements)

References 22 publications

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“…The oncogenic role of β-Catenin, which is believed to be due to upregulation of the canonical Wnt signaling pathway, has been very well studied in colon cancer and some other human malignancies (Polakis, 2000;Salmanian et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…β-Catenin is a dual function protein that in addition to its role as a component of cell adherens junction, intracellular and intranuclear localization of this protein are oncogenic and may help tumor initiation and progression (Cavallaro and Christofori, 2004;Salmanian et al, 2010). The oncogenic role of β-Catenin, which is believed to be due to upregulation of the canonical Wnt signaling pathway, has been very well studied in colon cancer and some other human malignancies (Polakis, 2000;Salmanian et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Anticancer Properties of Teucrium persicum in PC-3 Prostate Cancer Cells

Tafrihi

Toosi²,

Minaei³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Crude extracts or phytochemicals obtained from some plants have potential anti-cancer properties. Teucrium persicum is an Iranian endemic plant belonging to the Lamiaceae family which has traditionally been used to relieve abdominal pains. However, the anti-cancer properties of this species of the Teucrium genus have not been investigated previously. In this study, we have used a highly invasive prostate cancer cell line, PC-3, which is an appropriate cell system to study anti-tumor properties of plants. A methanolic extract obtained from T persicum potently inhibited viability of PC-3 cells. The viability of SW480 colon and T47D breast cancer cells was also significantly decreased in the presence of the T persicum extract. Flow cytometry suggested that the reduction of cell viability was due to induction of apoptosis. In addition, the results of wound healing and gelatin zymography experiments supported anti-cell invasion activity of T persicum. Interestingly, sublethal concentrations of T persicum extract induced an epithelial-like morphology in a subpopulation of cells with an increase in E-Cadherin and β-Catenin protein levels at the cell membrane. These results strongly suggest that T persicum is a plant with very potent anti-tumor activity

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anticancer Properties of Teucrium persicum in PC-3 Prostate Cancer Cells

Tafrihi

Toosi²,

Minaei³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…However, they may also directly affect the CatnB protein through interaction with their respective activated receptors. As Salmanian and colleagues have shown, expression of the M3-muscarinic acetylcholine receptor and subsequent activation of Gaq might stabilize CatnB protein via inhibition of GSK-3b (Salmanian et al, 2010). Insulin receptor activation may in turn stimulate phosphotidylinositide 3-kinase (PI3K) activity (reviewed by Cheng et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

NO/beta-catenin crosstalk modulates primitive streak formation prior to embryonic stem cell osteogenic differentiation

Ding

Keller

Martinez

et al. 2012

Journal of Cell Science

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SummaryNitric oxide (NO) has been shown to play a crucial role in bone formation in vivo. We sought to determine the temporal effect of NO on murine embryonic stem cells (ESCs) under culture conditions that promote osteogenesis. Expression profiles of NO pathway members and osteoblast-specific markers were analyzed using appropriate assays. We found that NO was supportive of osteogenesis specifically during an early phase of in vitro development (days 3-5). Furthermore, ESCs stably overexpressing the inducible NO synthase showed accelerated and enhanced osteogenesis in vitro and in bone explant cultures. To determine the role of NO in early lineage commitment, a stage in ESC differentiation equivalent to primitive streak formation in vivo, ESCs were transfected with a T-brachyury-GFP reporter. Expression levels of T-brachyury and one of its upstream regulators, b-catenin, the major effector in the canonical Wnt pathway, were responsive to NO levels in differentiating primitive streak-like cells. Our results indicate that NO may be involved in early differentiation through regulation of b-catenin and T-brachyury, controlling the specification of primitive-streak-like cells, which may continue through differentiation to later become osteoblasts.

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“…Heterodimerization of Fz proteins has also been proposed [69], which brings along further potential flexibility in ligand recognition and transduction. Furthermore, it is conceivable that Fz heterodimerization with other, non-Fz GPCRs may exist, which provides a possible explanation for the involvement of non-Gi/o heterotrimeric G proteins in Wnt pathways [28,30] (see below).…”

Section: Frizzleds As Gpcrs: View From Above the Surfacementioning

confidence: 99%

“…The first evidence which returned the missing G protein link in the chain of the Fz signal transduction was the role of pertussis toxin-sensitive G proteins obtained for the Fz-dependent non-canonical Ca 2+ -pathway in zebrafish [23]. Later the Go-protein was found to be a transducer in both canonical and PCP pathways in Drosophila [24][25][26], and roles for its orthologues along with other members of the heterotrimeric GTPase superfamily in Xenopus [27,28] and mammalian models [28][29][30][31] of bcatenin stabilization as well as cell polarization [32] were established. Although these findings suggested that Fz proteins were activators of G-proteins, only recently the final biochemical proof of the ability of Fz receptors to bind heterotrimeric Gproteins and activate the nucleotide exchange on them upon engaging the Wnt ligands has been provided [33][34][35] -the final demonstration that these receptors are bona fide GPCRs [36][37][38].…”

mentioning

confidence: 99%