Regulation of Hepatic 3‐Hydroxy‐3‐Methylglutaryl‐Coenzyme a Reductase

Hypocholesterolemic effect of physically refined rice bran oil: Studies of cholesterol metabolism and early atherosclerosis in hypercholesterolemic hamsters

“…After cholesterol absorption was measured during week 7, animals were euthanized and their livers and small intestines collected for measurement of HMG-CoA reductase activity. Animals were euthanized in the middle of the dark cycle (12/12-h light/ dark cycle) when HMG-CoA reductase was at the peak of its diurnal rhythm [34].…”

Evaluation of 3‐hydroxy‐3‐methylglutaryl‐COA‐reductase, cholesterol‐7α‐hydroxylase and acyl‐COA:cholesterol acyltransferase activities: alternative chromatographic methods to separate metabolites

“…Cholesterol metabolism is mainly regulated by three enzymes: 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoA-reductase, EC 1.1.1.34), cholesterol-7αhydroxylase (EC 1.14.13.17) and acyl-CoA:cholesterol acyltransferase (ACAT, EC 2.3.1.26). HMG-CoAreductase catalyses the reduction of HMG-CoA and acetate, in the presence of two NADPH molecules, to generate mevalonate acid, an important product involved in cholesterol synthesis (Rodwell et al, 1973). Human HMG-CoA-reductase is a single polypeptide chain of 888 amino acids (Istvan and Deisenhofer, 2000).…”