Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20

Liu, Gan; Zhou, Luting; Hai, Zhang; Chen, Rong; Zhang, Ye; Li, Ling; Lu, Jun; Jiang, Hui; Liu, Dong; Qi, Shasha; Jiang, Ying; Yin, Kai; Xie, Zhifang; Shi, Yuguang; Liu, Yong; Cao, Xuetao; Chen, Yu Xia; Zou, Da-jin; Zhang, Weiping J.

doi:10.1038/ncomms14824

Cited by 52 publications

(48 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the altered mRNA levels of these genes following HFD feeding were restored by LNS1 administration to HFD mice. In contrast, although HFD feeding enhanced the mRNA levels of Zbtb20, which regulates hepatic lipogenesis in part by regulating Chrebp expression (Liu et al 2017), LNS1 administration did not significantly affect hepatic expression of Zbtb20 in HFD mice. Next, we determined the effect of LNS1-CS on the expression of these SREBP genes in HepG2 cells in association with AMPK activity by using CpC.…”

Section: Figurementioning

confidence: 67%

Lactobacillus acidophilus NS1 attenuates diet-induced obesity and fatty liver

Park

Lee

Song

et al. 2018

Journal of Endocrinology

View full text Add to dashboard Cite

Obesity is a major threat to public health, and it is strongly associated with insulin resistance and fatty liver disease. Here, we demonstrated that administration of NS1 (LNS1) significantly reduced obesity and hepatic lipid accumulation, with a concomitant improvement in insulin sensitivity, in high-fat diet (HFD)-fed mice. Furthermore, administration of LNS1 inhibited the effect of HFD feeding on the SREBP-1c and PPARα signaling pathways and reduced lipogenesis with an increase in fatty acid oxidation in livers from HFD-fed mice. These LNS1 effects were confirmed in HepG2 cells and livers by treatment with LNS1 culture supernatant (LNS1-CS). Interestingly, AMPK phosphorylation and activity in the liver of HFD-fed mice were increased by administration of LNS1. Consistently, chemical inhibition of AMPK with compound C, a specific inhibitor of AMPK, dramatically reduced the effect of LNS1-CS on lipid metabolism in HepG2 cells and livers by modulating the SREBP-1c and PPARα signaling pathways. Furthermore, administration of LNS1 to HFD-fed mice signiﬁcantly improved insulin resistance and increased Akt phosphorylation in the liver, white adipose tissue and skeletal muscle. Together, these data suggest that LNS1 may prevent diet-induced obesity and related metabolic disorders by improving lipid metabolism and insulin sensitivity through an AMPK→SREBP-1c/PPARα signaling pathway.

show abstract

Section: Figurementioning

confidence: 67%

Lactobacillus acidophilus NS1 attenuates diet-induced obesity and fatty liver

Park

Lee

Song

et al. 2018

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Thus, ZBTB20 might be a functional target of miR-758-5p in GBM. ZBTB20, as a zinc finger protein, has been implicated in glucose and lipid homoeostasis [30,31] and in the tumor progression of hepatocellular carcinoma [32], indicating a role in cancer. In our study, ZBTB20 was overexpressed in GBM, and ZBTB20 knockdown suppressed GBM progression.…”

Section: Discussionmentioning

confidence: 99%

Mir-758-5p Suppresses Glioblastoma Proliferation, Migration and Invasion by Targeting ZBTB20

Jiang

Hui

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: To determine the cellular functions and clinical significance of micro-758-5p (miR-758-5p) in glioblastoma (GBM) by targeting zinc finger and BTB domain-containing protein 20 (ZBTB20). Methods: Fifty-five paired GBM tissues and adjacent normal tissues, GBM cell lines (U118, LN-299, H4, A172, U87-MG, and U251), and normal human astrocyte cell line (HEB) were used. miR-758-5p mimics, ZBTB20 siRNA, and pcDNA3.1-ZBTB20 were transiently transduced into cancer cells independently or together. qRT-PCR was conducted to analyze the expression of miR-758-5p and ZBTB20. Luciferase reporter assays were performed to determine the effect of miR-758-5p on ZBTB20. Western blot was applied to measure the expression of ZBTB20, PCNA, and cleaved caspase3. Cell Counting Kit-8 (CCK8), colony formation, FACS, and Transwell assays were carried out to detect cellular proliferation, apoptosis, migration, and invasion. Xenograft experiments were implemented to evaluate tumor growth and metastasis in vivo. Results: miR-758-5p was significantly downregulated in GBM tissues and cell lines compared with that in adjacent normal tissues and HEB cells. miR-758-5p overexpression inhibited the proliferation, migration, and invasion of GBM cells and induced apoptosis by regulating the ZBTB20 expression. Pearson correlation analysis also confirmed that miR-758-5p was inversely correlated with ZBTB20 in GBM tissues. miR-758-5p suppressed tumor growth and metastasis in vivo. The restored ZBTB20 expression partially rescued the miR-758-5p-induced inhibition of GBM cell proliferation, migration, and invasion. Kaplan–Meier curve analysis revealed that a high miR-758-5p expression indicated an enhanced prognosis of patients with GBM. Conclusion: miR-758-5p suppressed GBM progression by targeting ZBTB20. The miR-758-5p/ZBTB20 axis might be a promising therapeutic target for GBM treatment.

show abstract

“…Notably the differences in methylation levels according to the adherence to the Mediterranean diet was inversely correlated with differences in the gene expression of ZNF577. These (31) 80.0% (12) 67.4% (31) 81.3% (13) 65.8% (25) 79.2% (19) 66.7% (32) 85.5% (12) 69.7% (23) 71.4% (20) Data show the mean ± standard deviation. † Data show the percentage (sample size).…”

Section: Methylation and Expression Levels Of Znf577 Based On Adiposimentioning

confidence: 99%

“…The functional role of ZNF577 is unknown; however, some members of the zinc finger proteins (ZNFs) family, which regulate gene transcription, have been found to be often hypermethylated and silenced in different types of tumors, suggesting that it may represent a commonly disrupted epigenetic pathway in cancer progression (13). In a metabolic and nutritional context, family members of the ZNFs have been associated with adipogenesis (14)(15)(16), type 2 diabetes, insulin resistance (17,18), the regulation of hepatic lipogenesis (19,20), and in the control of muscle function (21). Moreover, the expression of some ZNFs is regulated by nutritional status such as fasting (22) or under a high-fat diet (23,24).…”

Section: Introductionmentioning

confidence: 99%

ZNF577 Methylation Levels in Leukocytes From Women With Breast Cancer Is Modulated by Adiposity, Menopausal State, and the Mediterranean Diet

et al. 2020

View full text Add to dashboard Cite

Lorenzo et al. Methylation, Adiposity, and Diet in Breast Cancer women with greater adherence to the Mediterranean diet (p = 0.017) or specific foods. Relevantly, the methylation levels of ZNF577 showed a good ability for fish consumption detection [area under the ROC curve (AUC) = 0.72; p = 0.016]. In conclusion, the association between methylation of ZNF577 and adiposity, menopausal state, and adherence to the Mediterranean diet can be detected in the blood leukocytes. The results guarantee the need of performing further studies in longer longitudinal cohorts in order to elucidate the role of ZNF577 methylation in the association between breast cancer, adiposity and dietary patterns.

show abstract

Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20

Cited by 52 publications

References 51 publications

Lactobacillus acidophilus NS1 attenuates diet-induced obesity and fatty liver

Lactobacillus acidophilus NS1 attenuates diet-induced obesity and fatty liver

Mir-758-5p Suppresses Glioblastoma Proliferation, Migration and Invasion by Targeting ZBTB20

ZNF577 Methylation Levels in Leukocytes From Women With Breast Cancer Is Modulated by Adiposity, Menopausal State, and the Mediterranean Diet

Contact Info

Product

Resources

About