Regulation of Histone Acetylation on Expression Profiles of Potassium Channels During Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells

Abstract

The cardiomyocyte differentiation from mouse embryonic stem cells (mESCs) is a dynamic and complex process that involved in the precision regulation of histone acetylation. The formation of action potential (AP) in mature cardiomyocytes is based on the expression pattern of Na , Ca , and K ion channels, in which the slow delayed rectifier potassium current (I ), the rapid delayed rectifier potassium current (I ) and the inwardly rectifying Kir current (I ) mainly contribute to repolarization for AP in different species. However, the expression status of potassium channels conducted I , I , and I in cardiomyocyte differentiation are not fully defined. Here, we investigated the expression pattern of the slow delayed rectifier potassium channel and the rapid delayed rectifier potassium channel using a model of mouse cardiomyocyte differentiation under different conditions of histone acetylation. We found that expression levels of both the delayed rectifier potassium channel and the inwardly rectifying potassium channel were more sensitive to histone hyperacetylation during differentiation from mESCs into cardiomyocytes. Especially, histone H4 hyperacetylation induced by Class I HDACs inhibitors promoted the expression profiles of potassium channels (Kcnj2, Kcnj3, Kcnj5, Kcnj11, and Kcnh2) in the process. Our results provide a clue for expression status of potassium channels which may be essential to forming functional cardiomyocyte in the cardiac lineage commitment of mESC. J. Cell. Biochem. 118: 4460-4467, 2017. © 2017 Wiley Periodicals, Inc.

Laminin promotes differentiation of rat embryonic stem cells into cardiomyocytes by activating the integrin/FAK/PI3K p85 pathway

“…45 Why matrigel and geltrex exert distinct effects on cardiomyogenesis of hESC and mESC? 20,47 Indeed, we provided evidence that laminin is the critical component of ECMs required for an optimal and efficient differentiation of rESC to cardiomyocytes. 46 Also the commercial geltrex matrix contains laminin, collagen IV, entactin and heparin sulphate proteoglycans.…”

“…Moreover, gelatin, a mixture of peptides and proteins extracted from the skin, bones and connective tissues of animals has been used as ECM to induce the differentiation of mESCs or hESCs into cardiomyocytes. 20,47 Indeed, we provided evidence that laminin is the critical component of ECMs required for an optimal and efficient differentiation of rESC to cardiomyocytes.…”

“…20 The details of method are described in Supplemental Experimental Procedures and the related primers are listed in Table S1. 20 The details of method are described in Supplemental Experimental Procedures and the related primers are listed in Table S1.…”

“…Quantitative real-time PCR (qRT-PCR) was performed as described previously. 20 The details of method are described in Supplemental Experimental Procedures and the related primers are listed in Table S1.…”