Regulation of histone H10 accumulation during induced differentiation of murine erythroleukemia cells

Quantitative analysis of histone H1° protein synthesis in HTC cells

“…Data reported using cell labeling, histone extraction and autoradiography of polyacrylamide gels, showed that induction of H18 synthesis occurred in a pulse with a peak at 6 h followed by a decrease to negligible levels. Similar experiments performed during HMBA-induced differentiation of MEL cells revealed that in this case the synthesis was increased by a factor 3.8 after 8 h of induction and then remained constant up to 24 h [7].…”

“…Data reported using cell labeling, histone extraction and autoradiography of polyacrylamide gels, showed that induction of H18 synthesis occurred in a pulse with a peak at 6 h followed by a decrease to negligible levels. Similar experiments performed during HMBA-induced differentiation of MEL cells revealed that in this case the synthesis was increased by a factor 3.8 after 8 h of induction and then remained constant up to 24 h [7].In this study, using a quantitative and sensitive method based on histone purification by ion-exchange HPLC, we attempted to measure H18 synthesis in HTC cells under different conditions of culture and to determine the relationship between the cellular mRNA content and the effective translation rate. …”

“…Because this effect is reversible upon removal of the inducer, a 24-h sodium butyrate treatment can therefore be used for HTC cell synchronization [5]. Experiments performed on MEL, B16 and HTC cells showed that H18 mRNA accumulation resulted from an increase of H18 transcription rate [3,4,7], which did not require protein synthesis when stimulated by either HMBA [7] [4]. In the MEL cell line, H18 mRNA preferentially accumulates in mid or late S phase, even when cells have been pulse-induced by a 2-h treatment with HMBA [12,13].…”

“…Treatment of MEL cells with HMBA [7], a rat-liver regeneration analysis [14] and a rat-brain development study [15] have provided evidence that in these model systems accumulation of H18 protein did not correspond to that expected from the amount of mRNA, suggesting that the translation level was finely regulated. Histone H18 synthesis has been studied during induced differentiation of B16 cells under sodium butyrate treatment [13,16].…”

An Upstream Control Region Required for Inducible Transcription of the Mouse H1° Histone Gene during Terminal Differentiation

“…Increased accumulation of H1Њ mRNA can be detected within 1 h of HMBA treatment, and the mRNA gradually accumulates, reaching a maximum level between 18 and 24 h, whereupon it is maintained at this high level throughout most of the differentiation program (11). Nuclear run-on experiments in MEL cell nuclei indicated that the induction is primarily controlled by increased transcription (9,62). To localize DNA sequences required for expression and induction of the H1Њ gene in differentiating MEL cells, we used a stable transfection strategy.…”