Regulation of human basophil activation

Beauvais, Francis; Bidet, B.; Descours, B.; Hiéblot, Corinne; Burtin, C.; Benveniste, Jacques

doi:10.1016/0091-6749(91)90426-o

Journal of Allergy and Clinical Immunology

1991

DOI: 10.1016/0091-6749(91)90426-o

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Regulation of human basophil activation

Francis Beauvais¹,

B. Bidet²,

B. Descours³

et al.

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Cited by 20 publications

(1 citation statement)

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“…Gramicidin D from Bacillus brevis increases the ionic permeability of numerous biological membranes ( − ), including the cytoplasmic membrane of Gram-positive and Gram-negative bacteria ( , ), the inner mitochondrial membrane () and the plasma membrane of erythrocytes ( − ), fibroblasts ( , ), skeletal muscle cells ( − ), and immune cells ( − ). Gramicidin is a pentadecapeptide of alternating d - and l -amino acids organized as a 6.3 β-helix (see ref 19 for a review).…”

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confidence: 99%

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells

et al. 1999

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Gramicidin D and alamethicin are pore-forming peptides which exhibit lethal properties against a large spectrum of cells. Despite a wealth of experimental data from artificial membranes, the time course and quantitative analysis of the activity of these ionophores are not well described in living cells. In the present study, the newly described fluorescent dye CD-222 was used to monitor extracellular potassium ion concentration and report the effects of these antibiotics on the K+ permeability of the plasma membrane of Spodoptera frugiperda (Sf9) and Choristoneura fumiferana (Cf1) insect cells. Both peptides induced a rapid efflux of intracellular K+ as a consequence of ion channel formation in the cell membrane. K+ efflux began without any measurable delay. While the final extracellular K+ concentration was unaffected by ionophore concentration, the rate of K+ efflux was dose dependent. Using a model describing the partition of the peptides in lipid membranes, the K+ efflux kinetic parameters were determined for both cell types and both pore formers. The proposed stoichiometry for the channel formed by gramicidin in living cells is in good agreement with the two-monomers model based on data from artificial membrane systems. The K+-permeable channel formed by alamethicin in insect cells appears to involve three monomers.

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confidence: 99%

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells

et al. 1999

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Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venom

Lambert¹,

Guilloux²,

Dzviga³

et al. 2003

Cytometry Part B Clinical

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Background: Flow cytometry (FCM) has been proposed for specific allergy in vitro testing. We investigated its biological significance for allergy to Hymenoptera venoms and compared it with the routinely performed basophil histamine release test (HRT).Methods: Blood samples from 26 allergic and 8 nonallergic donors were incubated with venom at serial concentrations. Basophils were analyzed with anti-CD45-PE-Cyanin 5, Anti-IgE-FITC, and Anti-CD63-Phycoerythrine. HRT was measured by radioimmunoassay.Results: FCM was as convenient as HRT for measuring basophil reactivity in at least 87% of allergic and 75% of nonallergic subjects. CD63 outer expression was specifically induced in 91% of releaser subjects (86% on HRT) and in 1 of 10 tests in nonallergic donors, or one of six tests (16% on HRT) in allergic patients tested with an irrelevant allergen. Both methods were concordant in 85.7% of the tests. The three discordant patients had low-grade reactions and borderline biological responses on FCM (n ‫؍‬ 2) or HRT (n ‫؍‬ 1).Conclusions: The dynamic, physiologic significance of CD63, the dose-response curve, and dependency on ethylene-diaminetetra acetic acid suggested that both tests reflect the same mechanism. Cytometry Part B (Clin. Cytometry) 52B:13-19, 2003.

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Allergic sensitization in infantile autism

Bidet

Leboyer

Descours

et al. 1993

J Autism Dev Disord

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Regulation of human basophil activation

Cited by 20 publications

References 17 publications

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells

Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venom

Allergic sensitization in infantile autism

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About

Regulation of human basophil activation

Cited by 20 publications

References 17 publications

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K+Efflux from Sf9 and Cf1 Insect Cells

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K+Efflux from Sf9 and Cf1 Insect Cells

Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venom

Allergic sensitization in infantile autism

Contact Info

Product

Resources

About

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells

Real-Time Fluorimetric Analysis of Gramicidin D- and Alamethicin-Induced K⁺Efflux from Sf9 and Cf1 Insect Cells