Regulation of Human Cytochrome P4501A1 (hCYP1A1): A Plausible Target for Chemoprevention?

Santes-Palacios, Rebeca; Ornelas-Ayala, Diego; Cabañas, Noel; Marroquín-Pérez, Ana L.; Hernández-Magaña, Alexis; Olguín-Reyes, Sitlali del Rosario; Camacho-Carranza, Rafael; Espinosa-Aguirre, J.J.

doi:10.1155/2016/5341081

Cited by 52 publications

(35 citation statements)

References 140 publications

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“…Changes in the expression levels of CYP1A1 caused by infections or inflammatory stimuli have been observed in various organs, including the liver, kidney, and brain [ 11 ]. CYP1A1 expression is upregulated in some cases but downregulated in other cases [ 11 , 21 ]. In this study, CYP1A1 gene expression significantly decreased in bovine mammary tissue and extracted epithelial cells under inflammatory conditions.…”

Section: Discussionmentioning

confidence: 99%

CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2018

Mediators of Inflammation

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The expression of cytochrome P4501A1 (CYP1A1) enzyme is changed in various organs during the host response to inflammation or infection, leading to alterations in the metabolism of endogenous and exogenous compounds. Results of this study showed that CYP1A1 expression was significantly downregulated in the mammary tissue of bovine with mastitis, in inflammatory epithelial cells (INEs) extracted from the tissue, and in lipopolysaccharide- (LPS-) induced INEs compared with their corresponding counterparts. Overexpression of CYP1A1 in bovine mammary epithelial cells alleviated the LPS-induced inhibition of epithelial proliferation, abated the LPS-induced increase of gene expression and protein secretion of inflammatory cytokine tumor necrosis factor-α and interleukin-6, and attenuated the LPS-induced activation of NF-κB signaling. These findings suggest that CYP1A1 has immense potential in the regulation of inflammatory responses in bovine mammary epithelial cells during mastitis and may serve as a useful therapeutic target in mitigating injuries caused by inflammatory overreaction.

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Section: Discussionmentioning

confidence: 99%

CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2018

Mediators of Inflammation

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“…Comprising seven exons and six introns, the CYP1A1 is located on 15q22-24 (Masson et al, 2005). It is generally expressed in extrahepatic tissues, especially epithelial tissues (Bozina et al, 2009;Santes-Palacios et al, 2016). Among CYP1A1 variant alleles, common variants include four single nucleotide polymorphisms (SNPs): T3801C (m1), A2455G (m2), T3205C (m3), and C2453A (m4) (Bozina et al, 2009).…”

Section: Research Articlementioning

confidence: 99%

Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women

Wongpratate

Ishida

Phuthong

et al. 2020

Asian Pac J Cancer Prev

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Background: CYP1A1 is an enzyme in phase I of the cytochrome P450 (CYP) superfamily, and plays a key role in detoxification of carcinogens. Host genetic predisposition in the CYP1A1 may be associated with an increased susceptibility to cervical cancer.The study aimed to evaluate four common polymorphisms of the CYP1A1 and cervical cancer susceptibility among Northeast Thai women. Methods: A case-control study was conducted involving 204 patients with squamous cell cervical cancer (SCCA) and 204 age-matched healthy controls. DNA was extracted from peripheral blood leucocytes. CYP1A1 m1, m3, and m4 genotypes were detected using PCR-RFLP, whereas the CYP1A1 m2 genotype was investigated using real-time PCR. Haplotype analysis was performed using PHASE algorithm version 2.1.1. Results: CYP1A1 m3 was monomorphic. Association between the common CYP1A1 polymorphisms, m1 and m2, and cervical cancer risk was not observed (p>0.05), nor was any association found between the m1-m2-m4 haplotype and cervical cancer risk (p>0.05). Interestingly, the CA genotype of CYP1A1 m4 was observed in 30.88% of the cervical cancer patients but was absent in healthy controls. Conclusion: Our results demonstrated a possible involvement of the CYP1A1 m4 polymorphism but no other common polymorphisms (viz., m1, m2, and m3) in the risk for cervical cancer.This finding may be useful when screening for risk of cervical cancer among Northeast Thai women.

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“…This amount is enough for some brain-CYP isoforms to play a role in tissue- and/or cell-specific sensitivity to certain xenobiotics [ 2 ]. However, the intracellular location is still a subject of study, since they seem to target either microsomes, mitochondria, or both [ 3 , 4 , 5 , 6 ]. Regardless of their location, some isoforms have been proposed to play an important role in neurodegeneration due to their xenobiotic metabolizing activity [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

β-Naphtoflavone and Ethanol Induce Cytochrome P450 and Protect towards MPP+ Toxicity in Human Neuroblastoma SH-SY5Y Cells

Fernandez-Abascal

Ripullone

Valeri

et al. 2018

IJMS

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Cytochrome P450 (CYP) isozymes vary their expression depending on the brain area, the cell type, and the presence of drugs. Some isoforms are involved in detoxification and/or toxic activation of xenobiotics in central nervous system. However, their role in brain metabolism and neurodegeneration is still a subject of debate. We have studied the inducibility of CYP isozymes in human neuroblastoma SH-SY5Y cells, treated with β-naphtoflavone (β-NF) or ethanol (EtOH) as inducers, by qRT-PCR, Western blot (WB), and metabolic activity assays. Immunohistochemistry was used to localize the isoforms in mitochondria and/or endoplasmic reticulum (ER). Tetrazolium (MTT) assay was performed to study the role of CYPs during methylphenyl pyridine (MPP+) exposure. EtOH increased mRNA and protein levels of CYP2D6 by 73% and 60% respectively. Both β-NF and EtOH increased CYP2E1 mRNA (4- and 1.4-fold, respectively) and protein levels (64% both). The 7-ethoxycoumarin O-deethylation and dextromethorphan O-demethylation was greater in treatment samples than in controls. Furthermore, both treatments increased by 22% and 18%, respectively, the cell viability in MPP+-treated cells. Finally, CYP2D6 localized at mitochondria and ER. These data indicate that CYP is inducible in SH-SY5Y cells and underline this in vitro system for studying the role of CYPs in neurodegeneration.

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Regulation of Human Cytochrome P4501A1 (hCYP1A1): A Plausible Target for Chemoprevention?

Cited by 52 publications

References 140 publications

CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells

CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells

Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women

β-Naphtoflavone and Ethanol Induce Cytochrome P450 and Protect towards MPP+ Toxicity in Human Neuroblastoma SH-SY5Y Cells

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