Regulation of human glioma cell apoptosis and invasion by miR-152-3p through targeting DNMT1 and regulating NF2

Sun, Jin; Tian, Xin; Zhang, Junqing; Huang, Yanlin; Xin, Lin; Chen, Luyue; Zhang, Shizhong

doi:10.1186/s13046-017-0567-4

Cited by 77 publications

(53 citation statements)

References 30 publications

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“…miR-152-3p is a member of the miR-148/152 family, 21 nucleotides in length, and is significantly suppressed in MM patients (32). A previous study found that miR-152-3p inhibited glioma cell proliferation and invasion activities by targeting DNMT1 (33). However, the role of miR-152-3p in MM is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA H19 Promotes Tumorigenesis of Multiple Myeloma by Activating BRD4 Signaling by Targeting MicroRNA 152-3p

Jian

Guo

et al. 2020

Molecular and Cellular Biology

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Multiple myeloma (MM) accounts for over twenty percent of hematological cancer-related death worldwide. Long noncoding RNA (lncRNA) H19 is associated with multiple tumorigenesis and is increased in MM, but the underlying mechanism of H19 in MM is unclear. In this study, the expression of H19, microRNA 152-3p (miR-152-3p), and BRD4 in MM patients was evaluated by quantitative real-time PCR (qRT-PCR) and Western blotting. Colony formation and flow cytometry analysis were used to determine the effects of H19 and miR-152-3p on MM cell proliferation, apoptosis, and cell cycle. A luciferase reporter assay was conducted to confirm the interaction among H19, miR-152-3p, and BRD4. A nude mouse xenograft model was established, and the cell proliferation and apoptosis were evaluated by immunohistochemistry (IHC) staining and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling assay. We found that levels of H19 and BRD4 were upregulated and the expression of miR-152-3p was downregulated in MM patients. Dual luciferase reporter assay showed H19 targeted miR-152-3p to promote BRD4 expression. Knockdown of H19 repressed proliferation and enhanced apoptosis and cell cycle G1 arrest by upregulating miR-152-3p in MM cells. Furthermore, H19 knockdown suppressed the growth of xenograft tumor, reduced Ki-67 and BRD4 levels, and increased cell apoptosis in xenograft tumor tissues. Taking these results together, H19 knockdown suppresses MM tumorigenesis via inhibiting BRD4-mediated cell proliferation through targeting miR-152-3p, implying that H19 is a promising biomarker and drug target for MM.

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Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA H19 Promotes Tumorigenesis of Multiple Myeloma by Activating BRD4 Signaling by Targeting MicroRNA 152-3p

Jian

Guo

et al. 2020

Molecular and Cellular Biology

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“…Furthermore, several studies have suggested that miR-152 is a tumor suppressor ( 34 ). miR-152 has been reported to inhibit cancer cell proliferation, invasion and migration by targeting Wnt1 ( 35 ), PIK3CA ( 36 ), DNMT1 ( 37 ) and PTEN ( 38 ). Additionally, Gu et al ( 39 ) identified that miR-152 promoted senescence in stem cells from human dental pulp by targeting SIRT7 expression.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of circular RNA expression in lung tissues from mice with hypoxia‑induced pulmonary hypertension

et al. 2018

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Pulmonary hypertension (PH) is a life-threatening lung disease, characterized by an increase in pulmonary arterial pressure caused by vasoconstriction and vascular remodeling. The pathogenesis of PH is not fully understood, and there is a lack of potential biomarkers for the diagnosis and treatment of patients with PH. Non-coding RNAs with a characteristic covalently closed loop structure, termed circular RNAs (circRNAs), are present in a number of pulmonary diseases. To the best of our knowledge, the present study is the first to use microarray analysis to determine the expression profile of circRNAs in lung tissues from mice with hypoxia-induced PH. In total, 23 significantly upregulated and 41 significantly down-regulated circRNAs were identified. Of these, 12 differentially expressed circRNAs were selected for further validation using reverse transcription-quantitative polymerase chain reaction. Putative microRNAs (miRNAs) that bind to the dysregulated circRNAs were predicted. Subsequently, bioinformatics tools were used to construct circRNA-miRNA-mRNA networks for the two most promising circRNAs, namely mmu_circRNA_004592 and mmu_circRNA_018351. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of target genes of the dysregulated circRNAs revealed that these dysregulated circRNAs may serve an important role in the pathogenesis of hypoxia-induced PH. Therefore, these dysregulated circRNAs are candidate diagnostic biomarkers and potential therapeutic targets for PH.

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“…Previous studies on the function of microRNA (miRNA/miR) have demonstrated that miRNAs are involved in the regulation of gene expression, cell differentiation, metabolism and invasion ( 19 , 20 ). Previous studies have indicated that miR-152-3p is closely associated with carcinogenesis, including glioma ( 21 – 23 ). Therefore, the present study aimed to explore the potential association between EWSAT1 and miR-152-3p in glioma.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR‑152‑3p

et al. 2020

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Long non-coding RNAs (lncRNA) are a type of ncRNA with a length ranging from 200-1,000 nucleotides. Previous studies have confirmed that the lncRNA Ewing sarcoma associated transcript 1 (EWSAT1) exerts regulatory roles in cancer development and progression. However, its clinical significance in glioma remains unknown. In the present study, RNA-sequencing data from the Gene Expression Omnibus database and The Cancer Genome Atlas was explored to investigate the association between EWSAT1 expression and prognosis in patients with glioma. Increased EWSAT1 was associated with the presence of necrosis on magnetic resonance imaging scans in patients with glioma. Furthermore, knockdown of EWSAT1 was indicated to suppress the proliferative and invasive abilities of glioblastoma cell lines using Cell Counting Kit-8 and Transwell assays. Additionally, microRNA (miR)-152-3p was identified as a potential target of EWSAT1. The present study demonstrated that EWSAT1 interacted directly with miR-152-3p, and rescue experiments confirmed that EWSAT1 participated in glioma development by suppressing miR-152-3p. These results indicated that EWSAT1 is involved in the occurrence and progression of glioma, and may serve as a novel target and potential prognostic biomarker of glioma treatment.

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Regulation of human glioma cell apoptosis and invasion by miR-152-3p through targeting DNMT1 and regulating NF2

Cited by 77 publications

References 30 publications

Long Noncoding RNA H19 Promotes Tumorigenesis of Multiple Myeloma by Activating BRD4 Signaling by Targeting MicroRNA 152-3p

Long Noncoding RNA H19 Promotes Tumorigenesis of Multiple Myeloma by Activating BRD4 Signaling by Targeting MicroRNA 152-3p

Characteristics of circular RNA expression in lung tissues from mice with hypoxia‑induced pulmonary hypertension

Long non‑coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR‑152‑3p

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