Regulation of IGF-I and porcine oviductal secretory protein (pOSP) secretion into the pig oviduct in the peri-ovulatory period, and effects of previous nutrition

Abstract

-The mechanisms regulating oviduct function were investigated. In Experiment 1, porcine oviductal secretory protein (pOSP) mRNA, and pOSP and insulin-like growth factor (IGF-I) in oviductal flushings, decreased through the peri-ovulatory period. In Experiment 2, higher plasma steroids in oviductal veins, ipsilateral (INT), rather than contralateral (OVX), to the remaining ovary in unilaterally ovariectomized gilts, were associated with higher pOSP in INT oviductal flushings. In Experiment 3, oviduct function was assessed as part of a collaborative study in cyclic gilts. Feed restriction in the late, compared to the early, luteal phase reduced estradiol concentrations in oviductal plasma, pOSP mRNA in oviductal tissue, and IGF-I concentrations and pOSP abundance in oviduct flushings. Previous insulin treatment differentially affected oviduct function. These data provide the first direct evidence for effects of previous feed restriction and insulin treatment on the oviduct environment in the peri-ovulatory period, which may contribute to nutritional effects on embryonic survival. oviduct / steroids / porcine oviduct secretory protein (pOSP) / IGF-I / pig Reprod. Nutr. Dev. 42 (2002) 355-372 355

The effect of gilt age at first estrus and breeding on third estrus on sow body weight changes and long-term reproductive performance1

“…The sensitivity of the assay was 0.1 ng/mL; the intraassay CV was 4.0%; and the interassay CV was 12.1%. Plasma IGF-1 concentrations were determined using the homologous double antibody RIA described by Novak et al (2002). One hundred microliters of plasma was initially extracted with 3 mL of acid ethanol.…”

Fecal and Urinary Lignans, Intrafollicular Estradiol, and Endometrial Receptors in Lactating Dairy Cows Fed Diets Supplemented with Hydrogenated Animal Fat, Flaxseed or Sunflower Seed

“…Concentrations of total IGF-I were also determined in follicular fluid samples (Days 5 and 15) by a homologous double antibody radioimmunoassay according to the method of Novak et al [24]. The antibody, rabbit anti-human-IGF-I serum (product name AFP4892898; gift from Dr. AF Parlow through the NIDDK's National Hormone and Pituitary Program, CA).…”

“…Transcervical uterine biopsy was performed [24] on Day 15 to collect endometrial samples in 15 animals. Briefly, after emptying the rectum, the vulva and perianal area was washed and disinfected with povidone iodine (Prepodyne solution; West Penetone, Anjou, QC, Canada).…”

“…Concentrations of total IGF-I were determined only in Day 5 plasma samples, by a homologous double antibody radioimmunoassay as previously described by Novak et al [24]. The interand intra-assay coefficients of variation were 17.7 and 5.6% and the sensitivity of the assay was 16.4 ng/ml.…”

Expression of KiSS-1 in rat oviduct: possible involvement in prevention of ectopic implantation?

“…These segments undergo dynamic morphological and physiological changes during the estrous cycle, which are driven by cyclic changes in ovarian steroids (Buhi 2002;Okada et al 2003). Indeed, the oviducts cannot be merely considered as ducts for the transport of the gametes and early embryos, but rather active secretory organs (Novak et al 2002;Buhi 2002;Killian 2004). Thus, the oviduct epithelium actively contributes to the formation of oviductal fluid, which is rich in electrolytes, enzymes, growth factors, and specific glycoproteins (Buhi 2002;Killian 2004).…”