Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells

Abstract

Québec-HSFA in Canada. His research team was internationally renowned for its major contributions to the understanding of the pathogenesis of endometriosis, as well as for its more recent studies on the molecular and cellular aspects of embryo implantation. He will be remembered fondly as a good friend, wonderful colleague, collaborator and mentor. AbstractThe mechanisms involving the expression of interleukin (IL) 1 family members in the process of preparing the endometrium to receive an embryo remain unclear. In this study, decidualization differentially skewed the balance of IL1 family receptor expression in a pattern that increases endometrial stromal cell receptivity to IL1, IL18 and IL33. Additionally, endometrial cells showed increased expression of homeobox HOXA10 and HOXA11 and LIFR, which are known to be involved in endometrial embryo receptivity. Further analyses of decidual endometrial cells revealed a significant increase in the release of potent proinflammatory, remodelling and angiogenic factors implicated in the embryo invasion process, such as VEGF (P = 0.0305), MMP9 (P = 0.0003), TIMP3 (P = 0.0001), RANTES (P = 0.0020), MCP1 (P = 0.0001) and MIF (P = 0.0068). No significant changes in endogenous IL1B secretion were observed. Decreased secretion of IL18 and decidualization increased secretion of IL33. These findings reveal a significant modulation of endometrial cell receptivity to IL1 family members during endometrial stromal cell decidualization, and suggest that the involvement of IL1 family members is important in physiological processes of endometrial receptivity, including adaptive immunology. This may be relevant to establishing a favourable uterine microenvironment for embryo implantation. Reproductive BioMedicine Online (2016) 32, 85-95 w w w. s c i e n c e d i r e c t . c o m w w w. r b m o n l i n e . c o m

Expression of proteins related to thyroid hormone function in the uterus is down‐regulated at the day of implantation in hypothyroid pregnant rats

“…Similar findings were also reported by Piccirilli et al (2018). It was also reported that in the uterus, expression of TRa and TRb were mainly localized to the macrophages and dendritic cells (De Vito et al, 2011), where these cells are involved in the implantation-related inflammatory processes (Bourdiec et al, 2016). Apart from the increased in TR expression in the endometrium, expression of TRa and TRb was also reported to increase in the blastocyst at the time of implantation (Colicchia et al, 2014).…”

“…Previous studies have shown that only sex-steroids, in particular progesterone is required to induce expression of these proteins in the uterus. It was also reported that in the uterus, expression of TRa and TRb were mainly localized to the macrophages and dendritic cells (De Vito et al, 2011), where these cells are involved in the implantation-related inflammatory processes (Bourdiec et al, 2016). Besides regulating these uterine proteins' expression, thyroid hormone (TH) has also been reported been reported to be essential for blastocyst development (Norwitz et al, 2001;Dey et al, 2004;Cheong et al, 2013) as well as has been reported to be involved in stimulating endothelial proliferation (Staun-Ram and Shalev, 2005;Demir et al, 2010) uterine tissue remodeling and angiogenesis (Dey et al, 2004;Plaisier, 2011).…”

Impact of local endometrial injury on in vitro fertilization/intracytoplasmic sperm injection outcomes: A systematic review and meta‐analysis

“…Thus, mechanical injury of the endometrium may enhance uterine receptivity by provoking the immune system to generate an inflammatory reaction . Acquisition of uterine receptivity is closely associated with an inflammatory response . In this meta‐analysis, we find that even diagnostic hysteroscopy can lead to positive effects on IVF/ICSI outcomes, albeit the endometrial injury minor compared with pipelle biopsy.…”

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

“…The interleukin 36 gamma gene (IL36G) is part of the interleukin 1 (IL1) cytokine family. During decidualization of human endometrial stromal cells, cell receptivity to the IL1 family is modulated [50], suggesting a role in endometrial receptivity. Endometrial expression of these three genes is stimulated by the presence of a more advanced conceptus, presumably as a result of specific factors expressed by the conceptus that help modulate the uterine environment to better support the development of a more advanced conceptus.…”