Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

Abstract

An abnormal uterine environment can influence maternal–fetal communication, conception rate and disrupt normal embryo development, thereby affecting fertility and the reproductive performance of dairy cows. Animal variability means that development of endometrial cell lines with appropriate characteristic are required. We evaluated the effect of an infectious agent (i.e., bacterial lipopolysaccharide; LPS) and proinflammatory mediators (i.e., Interleukin 1 beta; IL‐1β, and tumor necrosis factor alpha; TNFα) on inflammatory mediator gene expression and production by bovine endometrial epithelial (bEEL) and stromal (bCSC) cell lines. Expression of CXCL8/IL8, IL1A, IL1B, and IL6 cytokine genes was significantly upregulated in both epithelial and stromal cells when treated with LPS and IL‐1β. LPS treatment of epithelial cells (compared with treatment by IL‐1β and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Whereas, in stromal cells, IL‐1β treatment (compared with LPS and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Interestingly, bEEL and bCSC cells treated with IL‐1β increased IL1B gene expression, suggesting that IL‐1β may act unusually in an autocrine‐positive feedback loop. Cytokine production was stimulated by these agents in both cell types. We suggest that the characteristics of these two cell lines make them excellent tools for the study of intrauterine environment.

New models of lipopolysaccharide‐induced implantation loss reveal insights into the inflammatory response

“…66 Likewise, the TNF-α 308 promoter polymorphism, which is associated with high TNF-α production, has been linked to increased implantation rates and a reduced rate of miscarriage. [77][78][79][80] However, this study is one of the first to evaluate the direct response of hESCs to bacterial endotoxin exposure during decidualization. 69,70 Moreover, recent studies have demonstrated that G-CSF treatment in women with recurrent IVF failure improved implantation and pregnancy rates.…”

“…Induced cytokine expression in response to LPS has also been demonstrated in non-decidualized human, mouse, and bovine endometrial cells. [77][78][79][80] However, this study is one of the first to evaluate the direct response of hESCs to bacterial endotoxin exposure during decidualization. Interestingly, secreted levels of IL-12 and IFN-γ were significantly decreased in decidualized hESCs exposed to LPS compared to those that were not exposed to LPS.…”

Low‐level laser and adipose‐derived stem cells altered remodelling genes expression and improved collagen reorganization during tendon repair

“…9 A previous study of our group has shown the presence of transplanted ASCs on 3rd and 14th days after tendon transection, probably in response to the intense initial inflammatory process. B, Hydroxyproline concentration (mg/g tissue) of tendon RT: there was no significant difference between the experimental groups expression of Il1b, another proinflammatory cytokine, 40 it was observed a marked tendency to increase in the SC and L groups, corroborating with tendency to increase of Mmp8 gene expression in the same groups. No differences were observed in the expression of Tgfb1 among groups, but the higher concentration of IL-10 in the SC group corroborates the Our results do not demonstrate differences in the amount of TNF-α in the different groups, although the literature describes the anti-inflammatory role of LLL 38 and ASCs.…”

“…Significant differences between groups marked with the same letter (P < 0.05). B, Hydroxyproline concentration (mg/g tissue) of tendon RT: there was no significant difference between the experimental groups expression of Il1b, another proinflammatory cytokine, 40 it was observed a marked tendency to increase in the SC and L groups, corroborating with tendency to increase of Mmp8 gene expression in the same groups. Il1b has been identified as potent inducers of matrix metalloproteinases (MMPs).…”

Analysis of transcriptomic changes in bovine endometrial stromal cells treated with lipopolysaccharide

“…The researchers evaluated the effects of the LPS and pro-inflammatory mediators (IL-1β and TNFα) on bovine endometrial epithelial (bEEL) and stromal (bCSC) cell lines by detecting gene expression and production of cytokine and eicosanoid biosynthesis pathway. Result showed bEEL and bCSC are brilliant models in vitro for intrauterine environment studies [17,18]. Other study used primary isolated endometrial epithelial and stromal cells and ex vivo organ culture (EVOCs) as well-characterized models of endometritis to explore cellular pathways that modulate inflammatory responses within the endometrial tissue.…”