Regulation of Inhibin Subunit Gene Expression by Gonadotropins and cAMP in Ovarian Granulosa Cells

Mukherjee, Abir; Mayo, Kelly E.

doi:10.1007/978-1-59259-221-0_13

Cited by 1 publication

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“…Inhibin and activin can regulate FSH levels and, in turn, FSH can regulate expression of both inhibin ␣-subunit and ␤-subunits. It has been reported that when a 48-hr treatment with recombinant human FSH was given to cultured granulosa cells collected from 22-d-old rats, gene expression of inhibin ␣, ␤A, and ␤B subunits was significantly induced (82). In another study, FSH stimulates ␤A subunit production in cultured granulosa cells collected from 12-d-old rats (75).…”

Section: Discussionmentioning

confidence: 97%

Neonatal Exposure to Estrogens Suppresses Activin Expression and Signaling in the Mouse Ovary

et al. 2007

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In the ovary, the steroid hormone estrogen and the TGF-beta superfamily member activin are both produced by granulosa cells and they both have intraovarian functions. Emerging evidence has indicated an interaction of these two signaling pathways. Based on the fact that estrogen and activin can impact early follicle formation and development, we hypothesize that estrogen treatment may alter activin signaling in the neonatal ovary. Therefore, this study was designed to examine the effect of neonatal diethylstilbestrol (DES) and estradiol (E(2)) exposure on the mRNA and protein levels of the key factors involved in activin signaling in the mouse ovary. CD-1 mouse pups were given daily injections of DES, E(2), or oil on postnatal d 1-5, and ovaries and sera were collected on d 19. Neonatal DES or E(2) exposure decreased the number of small antral follicles, induced multioocytic follicle formation, and decreased activin beta-subunit mRNA and protein levels. Consistent with local loss of beta-subunit expression, the phosphorylation of Smad 2, a marker of activin-dependent signaling, was decreased in the estrogen-treated ovaries. The decreased beta-subunit expression resulted in a decrease in serum inhibin levels, with a corresponding increase in FSH. Estrogen also suppressed activin subunit gene promoter activities, suggesting a direct transcriptional effect. Overall, this study demonstrates that activin subunits are targets of estrogen action in the early mouse ovary.

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Section: Discussionmentioning

confidence: 97%