Regulation of intracellular pH in bovine oocytes and cleavage stage embryos

Lane, Michelle; Bavister, Barry D.

doi:10.1002/(sici)1098-2795(199912)54:4<396::aid-mrd10>3.0.co;2-6

Cited by 50 publications

(25 citation statements)

References 24 publications

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“…Similarly, HCO 3 À /Cl À exchange can be activated by alkalosis throughout preimplantation mouse embryo development (Baltz et al, 1991;Zhao et al, 1995;Zhao and Baltz, 1996;Cetinkaya and Erdogan, 2008), principally mediated by AE2 (Phillips and Baltz, 1999;Zhou et al, 2009). Similar findings have been reported for preimplantation embryos of human (Phillips et al, 2000), hamster (Lane et al, 1998(Lane et al, , 1999a, and bovine (Lane and Bavister, 1999). It had not yet been established that these exchangers can also function in cell volume regulation in preimplantation embryos.…”

Section: Acute Cell Volume Regulation By Inorganic Ion Transport In Esupporting

confidence: 85%

Cell volume regulation in mammalian oocytes and preimplantation embryos

Baltz

Zhou

2012

Molecular Reproduction Devel

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SUMMARYThe earliest stages of preimplantation embryos are particularly sensitive to increased osmolarity, even within the physiological range. This sensitivity contributed to persistent developmental arrest, even when embryos were cultured in vitro in older, conditioned culture media, and seems to arise when embryos at the 1-and 2-cell stages accumulate inorganic ions used for cell volume homeostasis at too high a level, through activation of coupled Na þ /H þ and HCO 3 À /Cl À exchange. Such accumulation of inorganic ions can be disruptive since, above a certain level, the increased ionic strength disrupts cellular biochemistry and macromolecular functions and alters membrane potential. To counter this, embryos have evolved mechanisms of cell volume regulation that are unique to early preimplantation embryogenesis. The primary role of these is glycine accumulation via the GLYT1 transporter, with a secondary contribution by betaine accumulation via the SIT1 transporter. Independent cell-volume regulation first arises in the oocyte only after ovulation is triggered, when the strong oocyte-zona pellucida adhesion present in germinal vesicle stage oocytes in the ovarian follicle is released and GLYT1 becomes activated to begin accumulating glycine. Open questions still remain regarding how these processes are regulated.Mol. Reprod. Dev. 79: 821-831, 2012. ß

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Section: Acute Cell Volume Regulation By Inorganic Ion Transport In Esupporting

confidence: 85%

Cell volume regulation in mammalian oocytes and preimplantation embryos

Baltz

Zhou

2012

Molecular Reproduction Devel

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“…Culture conditions affect more than metabolism as the ability of embryos to regulate intracellular pH is also affected by culture conditions. Mouse embryos are less sensitive to and recover more easily from changes in pH than either human or bovine embryos [11,19]. Thus, although the mouse played an important role in the pioneering work on embryo culture, the apparent differences among species and even among strains mean that caution should be exercised when extrapolating from one strain to another or from mice to other species.…”

Section: Biochemical/biophysicalmentioning

confidence: 99%

Virtues and limitations of the preimplantation mouse embryo as a model system

Taft

2008

Theriogenology

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The mouse is the most widely used model of preimplantation embryo development, but is it a good model? Its small size, prolificacy and ease of handling make the mouse a relatively low cost, readily available and attractive alternative when embryos from other species are difficult or expensive to obtain. However, the real power of the mouse as a model lies in mouse genetics. The development of inbred mouse strains facilitated gene discovery as well as our understanding of gene function and regulation while the development of tools to introduce precise genetic modifications uniquely positioned the mouse as a powerful model system for uncovering gene function. However, all models have limitations; the small size of the mouse limits tissue availability and manipulations that can be performed and differences in physiology among species may make it inappropriate to extrapolate from the mouse to other species. Thus, rather than extrapolating directly from the mouse to other species, it may be more useful to use the mouse as a model system for developing and refining hypotheses to be tested directly in species of interest. In this brief review, the value of the preimplantation mouse embryo as a model is considered, both as a model for other species and as a model for the mouse, as understanding the virtues and limitations of the mouse as a model system is essential to its appropriate use.

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“…Detailed studies on embryo metabolism, examination of tubal fluid composition and other physiologic, molecular and biochemical insights have led to improved modifications of media formulations. This has resulted in several excellent culture media, both mono and sequential systems, giving laboratories multiple choices in terms of selection of ingredients such as glucose concentration or amino acid complement [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. However, despite the number of options in regard to substrate selection for embryo culture media for use within the laboratory incubator, the ability to select from a variety of pH buffers in handling media for use outside the incubator is extremely limited.…”

Section: Introductionmentioning

confidence: 99%

“…External pH of culture media (pHe) influences sperm binding and motility [7][8][9], oocyte maturation [10,11] and embryo development [12][13][14][15][16], though confounding factors such as bicarbonate and CO 2 levels exist. Improper intracellular pH (pHi) can impede sperm function [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Improper intracellular pH (pHi) can impede sperm function [19][20][21][22]. Improper pHi is also detrimental to embryo development [23][24][25][26][27], impairs embryo metabolism [28,29], alters organelle localization [30], and even retards resulting fetal growth [31]. Furthermore, periodic fluctuations in environmental conditions, such as the pHe of media, can be harmful as these can be transduced into deleterious intracellular perturbations [32].…”

Section: Introductionmentioning

confidence: 99%

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Biological pH buffers in IVF: help or hindrance to success

Will

Clark

Swain

2011

J Assist Reprod Genet

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Purpose Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures Methods Keyword searches were performed using Pubmed and Medline and relevant literature reviewed. Results Various pH buffers have been used with varying degrees of success for gamete and embryo processing in a variety of animal species, as well as in human. Conclusion Though biologic buffers off a means to improve pH stability, not all buffers may be appropriate for use with gametes and embryos. Specific buffers may have undesired effects, and these may be buffer, species, cell type or concentration dependent. Continued research is needed to further refine and improve the use of biologic buffers for use in human ART.

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Regulation of intracellular pH in bovine oocytes and cleavage stage embryos

Cited by 50 publications

References 24 publications

Cell volume regulation in mammalian oocytes and preimplantation embryos

Cell volume regulation in mammalian oocytes and preimplantation embryos

Virtues and limitations of the preimplantation mouse embryo as a model system

Biological pH buffers in IVF: help or hindrance to success

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