Regulation of invertase of Actinomyces viscosus

Kiel, R. A.; Tanzer, Jason M.

doi:10.1128/iai.17.3.510-512.1977

Cited by 8 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A feedback regulation of the glycolytic products on the invertase activity is possible, but not proven in reports on invertases from cariogenic streptococci (19,28,30). Only one report on invertase from Aetinomyces viscosus (18) has shown that the enzyme activity was inhibited by fructose-6-phosphate and fructose-1,6-diphosphate. So thedeliberate elimination of the glycolytic pathway is presumably unimportant to the invertase activity measured, but necessary for an exact assay.…”

Section: Discussionmentioning

confidence: 99%

Method for determination of invertase acitivity in homogentes of human dental plaque

Fiehn

Moe

1981

European J Oral Sciences

View full text Add to dashboard Cite

– Supragingival human dental plaque was ecllected from patients with evidence of caries. The plaque was frozen and stored at‐20°C. Pooled plaque was homogenized in acelate buffer pH 5.0 in an ice‐water bath. By incubating the homogenate at pH 5.0 with [U‐°C]‐sucrose the formation fo glucose and fructose was followed. Incubation in acetate buffer at pH 5.0 eliminated the glycosyltransferase activities adn teh glycolytic pathway. Normal Michaclis‐Menten kinetics were observed until about 40 mM sucrose. At highert concentration of sucrose, excess substrate inhibition occurred. Storage of the homogenate at −20° C resulted in decrease of the invertase activity with time.

show abstract

Section: Discussionmentioning

confidence: 99%

Method for determination of invertase acitivity in homogentes of human dental plaque

Fiehn

Moe

1981

European J Oral Sciences

View full text Add to dashboard Cite

show abstract

Actinomycesand Related Genera

Funke¹

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

View full text Add to dashboard Cite

Actinomyces

Schaal¹,

Yassin²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Ac.ti.no.my'ces. Gr. fem. n. aktis, aktinos ray; Gr. masc. n. mukês fungus; N.L. masc. n. Actinomyces ray fungus referring to the radial arrangement of filaments in Actinomyces bovis sulfur granules. Actinobacteria / Actinobacteria / Actinomycetales / Actinomycetaceae / Actinomyces Straight or slightly curved rods , 0.2–1.0 µm in diameter, which vary considerably in shape and size, and slender filaments , 1 µm or less in width and 10–50 µm or more in length, with true branching. Short rods (0.5–5.0 µm in length) with or without clubbed ends are frequently seen and may occur singly, in pairs with diphtheroidal arrangements (Y, V, T forms and palisades), in short chains or in small clusters. Longer (5.0–10.0 µm in length) branched rods are also common. Several members of the genus appear chiefly or exclusively as coccobacillary or even coccoid elements. Filaments which may predominate in certain species are either straight or wavy , show varying degrees of branching and may have swollen, clubbed, or clavate ends. Gram‐stain‐positive , but irregular staining giving rise to a beaded or barred appearance frequently occurs, and two species may be Gram‐stain‐variable. Non‐acid‐fast, nonmotile, and non‐endospore‐forming. Conidia are not produced. DNA G+C content (mol%) : 55–71 ( T m , HPLC). Type species : Actinomyces bovis Harz 1877, 133 AL .

show abstract

Regulation of invertase of Actinomyces viscosus

Cited by 8 publications

References 16 publications

Method for determination of invertase acitivity in homogentes of human dental plaque

Method for determination of invertase acitivity in homogentes of human dental plaque

Actinomycesand Related Genera

Actinomyces

Contact Info

Product

Resources

About