Regulation of Key Enzymes in Lentinula Edodes Mycelium Culture by Chitosan

Abstract

Chitosan is a plant growth regulator. Since it has an important function, it may also play a similar role in the growth of fungi. In this study, Lentinula edodes was used as a model fungus, and the mycelium growth and metabolism in the cultivation bag treated with different concentrations of chitosan were investigated. The results showed when the chitosan concentration was 0.1 mg/ml, a dense mycelium was developed and the fast growth was observed. Compared with other concentrations and the control group, they had significant differences. Furthermore, the activity of CMCase, Amy, and POD were measured, which showed significant differences in the rapid growth of the mycelium compared with other concentrations. The concentrations of 0.1 mg/mL will be chosen to promote the growth of Lentinula edodes' mycelium in practical production.

A Comparative Study of Calcium Sulfate Alternatives in Compost Production for White Button Mushroom (Agaricus bisporus)

“…Interestingly, most such research did not aim to alter the technological bases but focused on enhancing the quality and nutritional value of the cultivated mushrooms [12]. Experiments were conducted with species including Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, and Hypsizygus marmoreus, utilizing a range of calcium sources for calcium accumulation [13][14][15]. Since the calcium sources were primarily used as additional supplements, a wide array of calcium sources was examined, such as calcium chloride, calcium amino acid chelates, calcium lactate, calcium nitrate, and calcium carbonate, as well as complex additives like agricultural lime, starfish powder, eggshells, and oyster shells, which predominantly contain calcium carbonate [16,17].…”