Regulation of Lactate Production by FSH, IL1β, and TNFα in Rat Sertoli Cells

Riera, María Fernanda; Meroni, Silvina Beatriz; Gómez, G.; Schteingart, Helena Fedora; Pellizzari, Eliana Herminia; Cigorraga, SB

doi:10.1006/gcen.2001.7619

Cited by 59 publications

(55 citation statements)

References 41 publications

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“…In Sertoli cells, increments in lactate production have been correlated with an increase in LDH activity which is accompanied by an increase in LDH A mRNA levels in several experimental models (Nehar et al 1997, 1998, Riera et al 2001. To further investigate biochemical steps that may be involved in the increase of lactate production by AMPK activation, experiments were designed to study the effect of AICAR on LDH activity and LDH A mRNA levels.…”

Section: Ldh Activity and Ldh A Expressionmentioning

confidence: 99%

The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-d-ribonucleoside, regulates lactate production in rat Sertoli cells

Galardo¹,

Riera²,

Pellizzari³

et al. 2007

Journal of Molecular Endocrinology

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The aim of the present study was to investigate whether the AMP-activated protein kinase (AMPK), a key regulator of cellular energy homeostasis, is present in Sertoli cells and whether its activation by 5-aminoimidazole-4-carboxamide-1-b-Dribonucleoside (AICAR) results in the regulation of cell metabolism to ensure lactate supply for germ cell development. Sertoli cell cultures from 20-day-old rats were used. Western blot analysis for the a-subunit of AMPK showed that high levels of AMPK are present in Sertoli cells. Treatment of the cultures with AICAR resulted in a dose-and time-dependent increase of P-AMPK levels indicating activation of the enzyme. A possible effect of AICAR on Sertoli cell lactate production was then analyzed. A dose-and time-dependent increment in lactate secretion was observed. The participation of AMPK activation in different biochemical processes that may be implicated in the regulation of lactate production was also analyzed. AICAR stimulated glucose uptake in a dose-and time-dependent manner. Additionally, AICAR increased the glucose transporter 1 (GLUT1) and decreased the glucose transporter 3 (GLUT3) mRNA levels. As for the role of AMPK in the regulation of the monocarboxylate transporters 1 and 4 (MCT1 and MCT4), it has been observed that AICAR treatment decreased MCT1 and increased MCT4 mRNA levels. In summary, the results presented herein show that AMPK is present in Sertoli cells and that its activation by AICAR increases lactate production as a result, at least in part, of a) an increase in glucose uptake, b) an increase in GLUT1 expression, and c) a decrease in MCT1 and an increase in MCT4 levels. Altogether, these results suggest an important role of AMPK in modulating the nutritional function of Sertoli cells.

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Section: Ldh Activity and Ldh A Expressionmentioning

confidence: 99%

The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-d-ribonucleoside, regulates lactate production in rat Sertoli cells

Galardo¹,

Riera²,

Pellizzari³

et al. 2007

Journal of Molecular Endocrinology

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“…The other biochemical variable, lactate is well known for its role in the regulation of spermatogenesis. It regulates spermatogenesis by serving as a transcription survival factor and participates in mitochondrial oxidative phosphorylation for the production of ATP which is required for developing germ cells [16].…”

Section: Discussionmentioning

confidence: 99%

In vitro studies on the effect of 8-(4-chlorophenylthio) cAMP on selected biochemical parameters in Sertoli cells isolated from testis of transgenic mice

Bhaskar¹

2013

iosrphr

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“…Several observations have been made on the regulation of lactate production by different locally produced factors. In this regard, EGF (Nehar et al 1993), IL1 (Nehar et al 1998) and TNF in porcine Sertoli cells (Nehar et al 1997) and insulin, insulin-like growth factor-I (Oonk et al 1989), EGF (Mallea et al 1986), PModS (Mullaney et al 1994), FSH and IL1 (Riera et al 2001) in rat Sertoli cells, act as regulators of lactate secretion. A distinct participation of these hormones on the stimulation of the different biochemical steps that will ultimately lead to increased lactate production has been observed.…”

Section: Discussionmentioning

confidence: 99%

“…Facilitated Sertoli cell glucose transport across plasma membrane is mediated by the carrier protein termed glucose transporter 1 (GLUT1), the only glucose transporter so far demonstrated in this cell (Ulisse et al 1992). As for the LDH isoenzyme system, increments in lactate production in Sertoli cells have been correlated with an increase in the LDH5 isoenzyme -containing four subunits A. Glucose transport through the plasma membrane and LDH A mRNA levels are regulated in a distinct manner by FSH (Riera et al 2001), interleukin 1 (IL1) (Nehar et al 1998), tumor necrosis factor-(TNF ) (Nehar et al 1997) and epidermal growth factor (EGF) (Boussouar & Benahmed 1999) -factors that modify Sertoli cell lactate production. No data are available on the mechanisms involved in the recently described effect of bFGF on lactate production (Schteingart et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Regulation of lactate production and glucose transport as well as of glucose transporter 1 and lactate dehydrogenase A mRNA levels by basic fibroblast growth factor in rat Sertoli cells

Riera

Meroni²,

Schteingart³

et al. 2002

Journal of Endocrinology

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By using cultured rat Sertoli cells as a model, both the action of basic fibroblast growth factor (bFGF) on lactate production and the site of this action were studied. bFGF stimulated Sertoli cell lactate production in a dosedependent manner (basal: 7·3 0·5; 0·1 ng/ml bFGF: 7·5 0·5; 1 ng/ml bFGF: 7·5 0·6; 10 ng/ml bFGF: 10·3 1·0; 30 ng/ml bFGF: 15·2 1·5; 50 ng/ml bFGF: 15·4 1·6 µg/µg DNA). Two major sites for the action of this growth factor were identified. First, bFGF was shown to exert short-and long-term stimulatory effects on glucose transport (basal: 1170 102; 30 ng/ml bFGF for 120 min: 1718 152 and basal: 718 64; 30 ng/ml bFGF for 48 h: 1069 69 d.p.m./µg DNA respectively). Short-term bFGF stimulation of glucose transport was not inhibited by the protein synthesis inhibitor cycloheximide. These results indicate that short-term bFGF stimulation of glucose uptake does not involve an increase in the number of glucose transporters. On the other hand, stimulation with bFGF for periods of time longer than 12 h increased glucose transporter 1 (GLUT1) mRNA levels. These increased mRNA levels were probably ultimately responsible for the increments in glucose uptake that are observed in long-term treated cultures. Secondly, bFGF increased lactate dehydrogenase (LDH) activity (basal: 31·0 1·4; 30 ng/ml bFGF: 45·7 2·4 mIU/µg DNA). The principal subunit component of those LDH isozymes that favors the transformation of pyruvate to lactate is subunit A. bFGF increased LDH A mRNA levels in a dose-and time-dependent manner. In summary, the results presented herein show that glucose transport, LDH activity and GLUT1 and LDH A mRNA levels are regulated by bFGF to achieve an increase in lactate production. These observed regulatory actions provide unequivocal evidence of the participation of bFGF in Sertoli cell lactate production which may be related to normal germ cell development.

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Regulation of Lactate Production by FSH, IL1β, and TNFα in Rat Sertoli Cells

Cited by 59 publications

References 41 publications

The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-d-ribonucleoside, regulates lactate production in rat Sertoli cells

The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-d-ribonucleoside, regulates lactate production in rat Sertoli cells

In vitro studies on the effect of 8-(4-chlorophenylthio) cAMP on selected biochemical parameters in Sertoli cells isolated from testis of transgenic mice

Regulation of lactate production and glucose transport as well as of glucose transporter 1 and lactate dehydrogenase A mRNA levels by basic fibroblast growth factor in rat Sertoli cells

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